Strategic Localization of Toll‐like Receptor 4 in the Digestive Tract of Blunt Snout Bream (Megalobrama amblycephala)

Zhang, G. Y.; Wang, Yonghu; Peng, Kaiman; Wang, C.; Tian, Guangming; Liu, H. Z.

doi:10.1111/j.1439-0264.2009.00950.x

Cited by 5 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The samples were then dissected into 6-m-wide sections and mounted on glass slides. All the slides were dried overnight at 37 ° C. To observe the morphological and structural changes of testes and ovaries, haematoxylin and eosin (HE) staining was used on the sections by using the method described by Zhang et al [2009].…”

Section: Haematoxylin and Eosin Stainingmentioning

confidence: 99%

See 1 more Smart Citation

SMARCE1 Promotes Chicken Embryonic Gonad Development by Regulating ERα and AR Expression

Gong

Yang²,

Liu³

et al. 2012

Sex Dev

View full text Add to dashboard Cite

SMARCE1 is one of some differentially expressed genes screened from a subtracted cDNA library between females and males during the period of sex differentiation. To understand the potential role of SMARCE1 in avian sex determination and differentiation, over-expression of SMARCE1 was performed in chicken embryos using the RCASBP.B retrovirus. Results showed that SMARCE1 expression was up-regulated in the infected embryonic gonads at the investigated stages (E6.5–E12.5) assessed by quantitative real-time RT-PCR, whole mount in situ hybridization and immunohistochemistry. With the over-expression of SMARCE1, CYP19A1, FOXL2, ERα, and SOX9 expression was significantly up-regulated while AR expression was significantly decreased in the male and/or female chicken gonad. Nevertheless, DMRT1 and AMH expression was not changed after the over-expression of SMARCE1. It is proposed that it might be via the SMARCE1-FOXL2 pathway that CYP19A1 expression was activated, and DMRT1 expression may be independent of the SMARCE1-SOX9 pathway. Meanwhile, the SMARCE1-AR pathway might be antagonized by up-regulated expression of ERα via estrogen-ER/androgen-AR signaling. The results of HE staining showed that the ovarian cortex was thickened and both testis seminiferous cord and interstitial cells were increased with the over-expression of SMARCE1. In conclusion, SMARCE1 can promote chicken embryonic gonad development by regulating the ERα and AR expression.

show abstract

Section: Haematoxylin and Eosin Stainingmentioning

confidence: 99%

“…The samples were dissected into serial 6-m-wide sections and mounted on poly-L -lysine-coated glass slides. All the slides were dried overnight at 37 ° C. A streptavidin-biotin-peroxidase complex immunohistochemical method [Zhang et al, 2009] was proceeded.…”

Section: Immunohistochemistrymentioning

confidence: 99%

SMARCE1 Promotes Chicken Embryonic Gonad Development by Regulating ERα and AR Expression

Gong

Yang²,

Liu³

et al. 2012

Sex Dev

View full text Add to dashboard Cite

show abstract

Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala

Zhu

Yang

Wang

et al. 2013

Fish Physiol Biochem

View full text Add to dashboard Cite

This study established and characterized a new cell line (MAF) from the fin of blunt snout bream (Megalobrama amblycephala), a freshwater fish cultivated in China. MAF cells proliferated well in medium 199 supplemented with 10 % fetal bovine serum at 28 °C and have been subcultured more than 95 times in almost a year. MAF cells were revived at 90-95 % viability after 3-6 months of storage in liquid nitrogen. Karyotyping indicated that the modal chromosome number of MAF cells was 48. The MAF cell line consisted predominantly of fibroblastic and epithelial-like cells from M. amblycephala, which was confirmed by immunofluorescence and mitochondrial 12s rRNA sequencing. Viral susceptibility tests showed that MAF cells were susceptible to infection by snakehead rhabdovirus, spring viremia carp virus, and channel catfish virus, which was demonstrated by the presence of cytopathic effect, high viral titers, and PCR products. Bacterial cytotoxicity studies showed that extracellular products from Aeromonas hydrophila were toxic to MAF cells. Cu²⁺ was also cytotoxic to MAF cells, and the 24-h IC₅₀ value was 144.48 μmol/l. When MAF cells were transfected with pEGFP-N1 plasmid, bright fluorescent signals were observed, and the transfection efficiency reached up to 5 %. These results suggest that the MAF cell line may provide a valuable tool for studying virus pathogenesis, as well as cytotoxicity testing and genetic manipulation studies.

show abstract

Distribution of mannose receptor in blunt snout bream (Megalobrama amblycephala) during the embryonic development and its immune response to the challenge of Aeromonas hydrophila

Zhao

Babu

et al. 2018

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Strategic Localization of Toll‐like Receptor 4 in the Digestive Tract of Blunt Snout Bream (Megalobrama amblycephala)

Cited by 5 publications

References 29 publications

<b><i>SMARCE1</i></b> Promotes Chicken Embryonic<b> </b>Gonad Development by Regulating<b><i> ER</i></b>α and <b><i>AR </i></b>Expression

<b><i>SMARCE1</i></b> Promotes Chicken Embryonic<b> </b>Gonad Development by Regulating<b><i> ER</i></b>α and <b><i>AR </i></b>Expression

Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala

Distribution of mannose receptor in blunt snout bream (Megalobrama amblycephala) during the embryonic development and its immune response to the challenge of Aeromonas hydrophila

Contact Info

Product

Resources

About