MORPHOLOGY AND PHYSIOLOGY OF
            <i>METHANOMONAS METHANOOXIDANS</i>

Stocks, Peter K.; McCleskey, C. S.

doi:10.1128/jb.88.4.1071-1077.1964

Cited by 35 publications

(13 citation statements)

References 8 publications

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“…Methane oxidation is observed in some environments such as soil and lakes, and methane-oxidizing bacteria, methanotrophs, have been detected in various environments and characterized (Auman et al 2000;Bull et al 2000). However, little is currently known about methanotrophs in the rumen (Stocks and McCleskey 1964). While approximately 20% of the net global methane flux is oxidized before it can enter the atmosphere (Prather et al 1995), a study using an artificial rumen indicated that only 0AE3% of methane flux is oxidized (Kajikawa and Newbold 2000).…”

Section: Introductionmentioning

confidence: 99%

Detection of Proteobacteria from the rumen by PCR using methanotroph-specific primers

Mitsumori

Ajisaka²,

Tajima

et al. 2002

Lett Appl Microbiol

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Aims: To detect Proteobacteria, including methanotrophs, from the rumen fluid and the bacteria inhabiting the rumen epithelium. Methods and Results: Proteobacteria inhabiting the rumen were detected by PCR using methanotroph-specific primers. The detected Proteobacteria were divided into clusters A, B, and C in addition to one clone, which was distinct from the clusters and closely related to Nitrosomonas sp. The clusters A, B, and C were close to Succinivibrio dextrinosolvens, Enterobacter cloacae, and Actinobacillus minor, respectively. The clones obtained from the rumen fluid each belonged to cluster A or B. The clones obtained from the rumen epithelium belonged to cluster B or C or to Nitrosomonas sp. Conclusions: It has been assumed that the rumen fluid and the rumen epithelium host different populations of Proteobacteria. Moreover, detection of Nitrosomonas from the rumen epithelium would indicate the possibility that the bacterium oxidizes ammonia and methane on the rumen surface. Significance and Impact of the Study: These findings suggest that the rumen fluid and the epithelium support different microbial populations, which would play specific roles in rumen function. Future study should focus on the relationship between these communities and physiological functions in the rumen.

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Section: Introductionmentioning

confidence: 99%

Detection of Proteobacteria from the rumen by PCR using methanotroph-specific primers

Mitsumori

Ajisaka²,

Tajima

et al. 2002

Lett Appl Microbiol

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“…Methane-utilising bacteria have been reported to grow poorly when methanol is the sole source of carbon and energy [1][2][3]. This has been attributed to the accumulation of formaldehyde [4], although the presence of this compound in the culture supernarant of methanol-grown cultures has been observed only when sodium sulphite was used as a trapping agent for formaldehyde [5].…”

Section: Introductionmentioning

confidence: 99%

Growth of the methane utilising bacterium Methylococcus NCIB 11083 in mineral salts medium with methanol as the sole source of carbon

Linton¹

1978

FEMS Microbiology Letters

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“…as sole sources of carbon and energy. Facultative methylotrophs can use compounds containing no carbon-carbon bonds as well as complex organic compounds with carbon-carbon bonds as sole sources of carbon and energy (6).Almost all methane-utilizing bacteria isolated in pure culture exhibit obligate growth requirement for methane, methanol, or dimethylether as a source of carbon and energy (5,9,11,19,32,35). Organic compounds were unable to serve as a carbon and energy source.…”

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confidence: 99%

Microbial Oxidation of Methane and Methanol: Isolation of Methane-Utilizing Bacteria and Characterization of a Facultative Methane-Utilizing Isolate

1978

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A methane-utilizing organism capable of growth both on methane and on more complex organic substrates as a sole source of carbon and energy, has been isolated and studied in detail. Suspensions of methane-grown cells of this organism oxidized C-1 compounds (methane, methanol, formaldehyde, formate); hydrocarbons (ethane, propane); primary alcohols (ethanol, propanol); primary aldehydes (acetaldehyde, propionaldehyde); alkenes (ethylene, propylene); dimethylether; and organic acids (acetate, malate, succinate, isocitrate). Suspensions of methanolor succinate-grown cells did not oxidize methane, ethane, propane, ethylene, propylene, or dimethylether, suggesting that the enzymatic systems required for oxidation of these substrates are induced only during growth on methane. Extracts of methane-grown cells contained a particulate reduced nicotinamide adenine dinucleotide-dependent methane monooxygenase activity. Oxidation of methanol, formaldehyde, and primary alcohols was catalyzed by a phenazine methosulfatelinked, ammonium ion-requiring methanol dehydrogenase. Oxidation of primary aldehydes was catalyzed by a phenazine methosulfate-linked, ammonium ionindependent aldehyde dehydrogenase. Formate was oxidized by a nicotinamide adenine dinucleotide-specific formate dehydrogenase. Extracts of methane-grown, but not succinate-grown, cells contained the key enzymes of the serine pathway, hydroxypyruvate reductase and malate lyase, indicating that the enzymes of C-1 assimilation are induced only during growth on C-1 compounds. Glucose-6-phosphate dehydrogenase was induced during growth on glucose. Extracts of methanegrown cells contained low levels of enzymes of the tricarboxylic acid cycle, including a-keto glutarate dehydrogenase, relative to the levels found during growth on succinate.Among the methylotrophic organisms are both obligate and facultative methylotrophs. Obligate methylotrophs are capable of utilizing only carbon compounds containing no carboncarbon bonds (methane, methanol, dimethylether, methylamine, etc.) as sole sources of carbon and energy. Facultative methylotrophs can use compounds containing no carbon-carbon bonds as well as complex organic compounds with carbon-carbon bonds as sole sources of carbon and energy (6).Almost all methane-utilizing bacteria isolated in pure culture exhibit obligate growth requirement for methane, methanol, or dimethylether as a source of carbon and energy (5,9,11,19,32,35). Organic compounds were unable to serve as a carbon and energy source. Recently, Patt et al. (25) reported the isolation and characterization of bacteria in pure culture that utilize methane as well as the complex organic compounds as sources of carbon and energy.In this paper we report on the isolation of both obligate and facultative methane-utilizing bacteria and on the characterization of one of the facultative isolates.MATERIALS AND METHODS Bacterial strain. Methylobacterium organophilum strain xx (ATCC 27886) (27) was obtained from the American Type Culture Collection, Rockvile, Md. Media a...

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MORPHOLOGY AND PHYSIOLOGY OF METHANOMONAS METHANOOXIDANS

Cited by 35 publications

References 8 publications

Detection of Proteobacteria from the rumen by PCR using methanotroph-specific primers

Detection of Proteobacteria from the rumen by PCR using methanotroph-specific primers

Growth of the methane utilising bacterium Methylococcus NCIB 11083 in mineral salts medium with methanol as the sole source of carbon

Microbial Oxidation of Methane and Methanol: Isolation of Methane-Utilizing Bacteria and Characterization of a Facultative Methane-Utilizing Isolate

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