Growth of the methane utilising bacterium Methylococcus NCIB 11083 in mineral salts medium with methanol as the sole source of carbon

Linton, J

doi:10.1016/0378-1097(78)90042-3

Cited by 10 publications

(16 citation statements)

References 9 publications

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“…In contrast, other workers have demonstrated the retention of methane-oxidizing activity in Type I methylotrophs (Linton & Vokes, 1978;Hyder et al, 1979). Our results contrast with those obtained by Hou et al.…”

Section: Discussioncontrasting

confidence: 86%

“…Despite the widely reported toxicity of methanol to obligate methylotrophs (Leadbetter & Foster, 1958;Stocks & McCleskey, 1964;Whittenbury et al, 1970a), growth on methanol at high concentrations is clearly possible for Methylocystis parvus OBBP (up to 4%, w/v; Hou et al, 1979a), MethyZococcus NCIB 11083 (up to 0-2%, v/v; Linton & Vokes, 1978), and Methylosinus trichosporium OB3b (up to 4%, v/v; this paper). The mechanism by which these organisms adapt to growth on methanol at high concentrations is unknown but may reflect physiological adaptation of the population to the substrate or the selection of a mutant resistant to either methanol or formaldehyde.…”

Section: Discussionmentioning

confidence: 91%

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Methane-oxidizing Activity and Membrane Morphology in a Methanolgrown Obligate Methanotroph, Methylosinus trichosporium OB3b

Best¹,

Higgins²

1981

Microbiology

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The obligate methylotroph Methylosinus trichosporium OB3 b was capable of growth on methanol as sole carbon source at concentrations as high as 4% (v/v), and viability was maintained over many successive transfers. Methane mono-oxygenase, detected by epoxidizing and hydroxylating activity, was retained. The gross morphology of the organism on this substrate was dependent on culture conditions. It varied from organisms containing extensive peripheral membrane systems to those with extensive inclusions, the latter representing a poorly developed membrane system which predominated under most growth conditions.

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Section: Discussioncontrasting

confidence: 86%

Section: Discussionmentioning

confidence: 91%

Methane-oxidizing Activity and Membrane Morphology in a Methanolgrown Obligate Methanotroph, Methylosinus trichosporium OB3b

Best¹,

Higgins²

1981

Microbiology

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“…A45. Methylocystis parvus OBBP was adapted to growth on methanol in chemostat culture as previously described (Linton & Vokes 1978;Prior & Dalton 1985). The methanol adapted strain is called M. parvus OBBPM.…”

Section: Strains and Growth Conditionsmentioning

confidence: 99%

Trichloroethylene oxidation by the membrane-associated methane monooxygenase in type I, type II and type X methanotrophs

et al. 1992

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Trichloroethylene (TCE) oxidation was examined in 9 different methanotrophs grown under conditions favoring expression of the membrane associated methane monooxygenase. Depending on the strain, TCE oxidation rates varied from 1 to 677 pmol/min/mg cell protein. Levels of TCE in the reaction mixture were reduced to below 40 nmolar in some strains. Cells incubated in the presence of acetylene, a selective methane monooxygenase inhibitor, did not oxidize TCE.Cultures actively oxidizing TCE were monitored for the presence of the soluble methane monooxygenase (sMMO) and membrane associated enzyme (pMMO). Transmission electron micrographs revealed the cultures always contained the internal membrane systems characteristic of cells expressing the pMMO. Naphthalene oxidation by whole cells, or by the cell free, soluble or membrane fractions was never observed. SDS denaturing gels of the membrane fraction showed the polypeptides associated with the pMMO. Cells exposed to ~4C-acetylene showed one labeled band at 26 kDa, and this protein was observed in the membrane fraction. In the one strain examined by EPR spectroscopy, the membrane fraction of TCE oxidizing cells showed the copper complexes characteristic of the pMMO. Lastly, most of the strains tested showed no hybridization to sMMO gene probes. These findings show that the pMMO is capable of TCE oxidation; although the rates are lower than those observed for the sMMO.

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“…However, an intermediate value could also have arisen from a low level of ammonia oxidation by the MMO which would serve to decrease the observed yield and increase the effective O2/CH4 ratio. Using Methylococcus NCIB 11083, a strain which responds to copper stress in an identical fashion to M. capsulatus (Bath) (Leak and Dalton, unpublished resuits), Linton and Vokes (1978) measured a CCE value of 46--47.8% in a similar growth medium under methane limitation. Their copper to biomass ratio was considerably lower than that utilized by Leak and Dalton (1986) thus reducing the potential contribution from particulate MMO.…”

Section: Soluble Mmomentioning

confidence: 99%

Growth yields of methanotrophs 2. A theoretical analysis

Leak

Dalton

1986

Appl Microbiol Biotechnol

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Growth of the methane utilising bacterium Methylococcus NCIB 11083 in mineral salts medium with methanol as the sole source of carbon

Cited by 10 publications

References 9 publications

Methane-oxidizing Activity and Membrane Morphology in a Methanolgrown Obligate Methanotroph, Methylosinus trichosporium OB3b

Methane-oxidizing Activity and Membrane Morphology in a Methanolgrown Obligate Methanotroph, Methylosinus trichosporium OB3b

Trichloroethylene oxidation by the membrane-associated methane monooxygenase in type I, type II and type X methanotrophs

Growth yields of methanotrophs 2. A theoretical analysis

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