Morphology and morphometry of the human sublingual glands in mouth floor enlargements of edentulous patients

de, Josiane Costa Rodrigues; Tolentino, Elen de Souza; Azevedo‐Alanis, Luciana Reis; Filho, Liogi Iwaki; Lara, Vanessa Soares; Damante, José Humberto

doi:10.1590/1679-775720130342

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…We found no studies relating the autolysis process and the aging, even because this phenomenon is usually investigated in postmortem studies and not in works related to senile changes. It seems evident that the process of autolysis is not exclusively a postmortem event, since Sá, et al 14 (2013) found this phenomenon in patients in vivo . However, association with the aging was not investigated by the authors 14 .…”

Section: Discussionmentioning

confidence: 96%

“…It seems evident that the process of autolysis is not exclusively a postmortem event, since Sá, et al 14 (2013) found this phenomenon in patients in vivo . However, association with the aging was not investigated by the authors 14 . We believe that autolysis integrates a set of changes already proven in the salivary glands of the elderly.…”

Section: Discussionmentioning

confidence: 96%

“…Age-related microscopic changes have been reported for the major 2 , 5 , 6 , 8 , 11 , 19 and minor 15 , 18 , 21 , 22 salivary glands of humans 2 , 5 , 8 , 14 , 15 , 18 , 19 , 21 , 22 and rats 6 , 11 . Main microscopic aspects described were: replacement of parenchyma by fat and connective tissue 2 , 8 , 15 , 21 , acinar atrophy and increase in the number of duct/duct-like structures 2 , 6 , 8 , 15 , 19 , 21 , focal and diffuse mononuclear infiltration 2 , oncocytosis 2 , 8 , 15 , and congested blood vessels 2 .…”

Section: Introductionmentioning

confidence: 95%

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Acinar autolysis and mucous extravasation in human sublingual glands: a microscopic postmortem study

et al. 2015

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Although some morphological investigations on aged human sublingual glands (HSG) found eventual phenomena identified as autolysis and mucous extravasation, the exact meaning of these findings has not been elucidated. Objective The aim of this work is to investigate whether acinar autolysis and mucous extravasation are related to the aging process in human sublingual glands. We also speculate if autolytic changes may assist forensic pathologists in determining time of death.Material and Methods 186 cadavers’ glands were allocated to age groups: I (0–30 years); II (31–60), and III (61–90). Time and mode of death were also recorded. Acinar autolysis and mucous extravasation were classified as present or absent. Ultrastructural analysis was performed using transmission electron microscopy (TEM). Data were compared using Mann-Whitney U, Spearman’s correlation coefficient, Kruskal-Wallis, and Dunn tests (p<0.05).Results There was correlation between age and acinar autolysis (r=0.38; p=0.0001). However, there was no correlation between autolysis and time of death. No differences were observed between genders. TEM showed mucous and serous cells presenting nuclear and membrane alterations and mucous cells were more susceptible to autolysis.Conclusion Acinar autolysis occurred in all age groups and increased with age while mucous extravasation was rarely found. Both findings are independent. Autolysis degrees in HSG could not be used to determine time of death.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 95%

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Acinar autolysis and mucous extravasation in human sublingual glands: a microscopic postmortem study

et al. 2015

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Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study

et al. 2015

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There are several age-related microscopic changes in the salivary glands, including the increase in the number of duct-like structures (DLS). However, the true origin and the phenotype of the DLS are not known. Objective To evaluate the phenotype and the cell proliferation index of the DLS of human sublingual glands.Material and Methods Sixty sublingual glands obtained from human cadavers were divided into two groups - 0-30 and 61-90 years old. The phenotype was estimated by immunostaining for cytokeratin 19 (CK 19) and the S-100 protein as well as by the presence of mucin and glycogen. The cell proliferation index was determined by the Ki-67 antibody. The histochemical techniques used periodic acid-Schiff (PAS) and Alcian Blue. In each captured microscopic field, the DLS were counted to establish a percentage for the staining profile. The statistical analysis was accomplished using Student’s t-test, the Mann-Whitney test and Pearson’s correlation coefficient (p<0.05).Results Comparing both groups, only CK 19 showed a statistically significant difference (p=0.033), with the strongest expression in the elderly group. There was no significant difference between PAS and Alcian Blue (p=0.270). In both groups, the immunostaining for CK 19 was stronger than that for S-100 (p=0.004;p<0.001), but there was no correlation between the two immunomarkers (ρ=-0.163; p=0.315). There was no immunostaining for Ki-67.Conclusions DLS demonstrate a ductal phenotypic profile and do not present cell proliferation activity. DLS may represent a regressive process arising from acini or represent the result of metaplasia.

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Histological and Immunohistochemical Study of Selenium Regenerative Effect on Submandibular and Sublingual Glands of Aging Rats

Elsaied

2019

Egyptian Dental Journal

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Background:The role of Selenium as an antioxidant has become a point of interest for its importance in prevention of cancer and other systemic diseases especially those associated with aging. Objectives:The aim of the present investigation was to investigate the effect of Selenium supplementation on the submandibular and sublingual salivary glands of aging rats through:1-Histological examination. 2-Immunohistochemical localization of Ki67. Materials and Methods:Thirty young adult male albino rats with body weight ranged from 80-110 gram and of 2 month age were used and divided into the following groups. Group I (control group): Consisted of 20 rats, subdivided into 2 subgroups. Group I.1:10 animals were sacrificed after one month to serve as young controls. Group I.2:10 animals were left till the end of the experiment to serve as old controls. Group II: 10 animals received Selenium 150 µg/kg diet for the whole experimental period. The experiment lasted for 9 months.Results: histological results revealed that aging caused devastating effects on the histological structure of submandibular and sublingual salivary glands. Selenium markedly modulated the action of aging process and increased the regenerative capacity of submandibular and sublingual glands. According to expression intensity of Ki67 stain in submandibular and sublingual glands, there was statistically significant difference between young, selenium and old groups. In general, expression intensity of Ki67 stain was the highest in the young control group followed by selenium group and lowest in old control group. Also, ki67 expression was significantly higher in sublingual than submandibular glands. Conclusions:Selenium was documented to have a powerful and beneficial antiaging effect. This faced lights around the promising role of selenium in the new era of regenerative medicine. Sublingual gland was more resistant to regressive changes of aging than submandibular gland.

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Morphology and morphometry of the human sublingual glands in mouth floor enlargements of edentulous patients

Cited by 3 publications

References 18 publications

Acinar autolysis and mucous extravasation in human sublingual glands: a microscopic postmortem study

Acinar autolysis and mucous extravasation in human sublingual glands: a microscopic postmortem study

Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study

Histological and Immunohistochemical Study of Selenium Regenerative Effect on Submandibular and Sublingual Glands of Aging Rats

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