Morphology and function of cryopreserved whole ovine ovaries after heterotopic autotransplantation

Grazul-Bilska, Anna T.; Banerjee, Jashoman; Yazici, Ilker; Borowczyk, Ewa; Bilski, Jerzy J.; Sharma, Rakesh K.; Siemionov, Maria; Falcone, Tommaso

doi:10.1186/1477-7827-6-16

Cited by 40 publications

(26 citation statements)

References 52 publications

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“…6). Apoptotic cells were also detected in ovine luteal tissues serving as a control (data not shown; see Vonnahme et al 2006, Grazul-Bilska et al 2008. Appearance of apoptotic cells in fetal ovaries was not affected by nutritional treatment.…”

Section: Resultsmentioning

confidence: 72%

Cellular proliferation and vascularization in ovine fetal ovaries: effects of undernutrition and selenium in maternal diet

Grazul-Bilska¹,

Caton²,

Arndt³

et al. 2009

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Sheep were fed a maintenance (M) diet with adequate (A) Se or high (H) Se concentration from 21 days before breeding to day 135 of pregnancy. From day 50 to day 135 of pregnancy (tissue collection day), a portion of the ewes from ASe and HSe groups were fed restricted (R; 60% of M) diet. Fetal ovarian sections were stained for: 1) the presence of proliferating cell nuclear antigen (a marker of proliferating cells) to determine the proportion of proliferating primordial follicles, or the labeling index (LI; percentage of proliferating cells) for primordial, primary, secondary and antral follicles, stromal tissues, and blood vessels; 2) factor VIII (a marker of endothelial cells) or 3) a presence of apoptotic cells/bodies. The number of proliferating primordial follicles and the LI of primordial follicles was decreased by R and/or HSe diets. The LI was similar for theca and granulosa cells, and for secondary or antral follicles, but was greater in secondary and antral than in primordial and primary follicles. R diet and/or Se affected the LI in all follicle types, in stromal tissues and blood vessels. A dense network of blood vessels was detected in the areas containing secondary to antral follicles, medulla, and hilus, but areas containing primordial follicles were poorly vascularized. The number of apoptotic cells was minimal. These results demonstrate that nutrient restriction and/or Se level in the maternal diet affected cellular proliferation in follicles, blood vessels, and stromal tissues in fetal ovaries. Thus, plane of nutrition and Se in the maternal diet may impact fetal ovarian development and function.

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Section: Resultsmentioning

confidence: 72%

Cellular proliferation and vascularization in ovine fetal ovaries: effects of undernutrition and selenium in maternal diet

Grazul-Bilska¹,

Caton²,

Arndt³

et al. 2009

Reproduction

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“…So far, encouraging results have been achieved. Normal oocytes and even offspring have been obtained from the grafts (Gosden et al 1994, Liu et al 2000, Snow et al 2002, Waterhouse et al 2004, Hasegawa et al 2006, Grazul-Bilska et al 2008, Hosoe et al 2008. Previously, ovaries were often transferred into the renal capsule (Liu et al 2000, Waterhouse et al 2004, Hosoe et al 2008, where the graft could easily re-vascularize.…”

Section: Discussionmentioning

confidence: 99%

Follicle growth and oocyte development after ovary transplantation into back muscle of immune-intact adult castrated male mice

Li¹,

Tao²,

Zhang³

et al. 2010

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Ovary grafting is not only a method of investigating follicle and oocyte development, but also a useful model to explore the possibility of the re-establishment of the reproductive axis in male-to-female sexual reversal. This study investigated ovary survival and follicle development after mouse ovaries were transplanted into immune-intact castrated male mice. Ten-day-old mouse ovaries were transplanted into the back muscle of adult outbred castrated male mice treated with immunosuppressants. Twenty-two days later, the ovary structure and the number of follicles present was examined by hematoxylin and eosin staining. The oocytes were harvested, and then used for in vitro maturation (IVM) and IVF. The results showed that primordial and antral follicles were mainly found in the grafts, and there were obvious differences compared with 32-day-old fresh ovaries (P!0.05). Embryos were derived from collected oocytes after IVM and IVF with a 72.4% cleavage rate and 7.9% blastocyst rate; 12 live pups were generated by embryo transfer. The hormone assay showed that plasma concentrations of both estrogen and progesterone increased after ovarian transplantation (P!0.01). In conclusion, immune-intact adult castrated male mice can support ovary survival and further development of follicles with endocrine function after ovarian transplantation.

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“…Reproductive organs are highly sensitive to injury from chemotherapy and radiation, often leaving survivors of childhood and young adult cancer infertile and with altered endocrine function, resulting in lifelong sexual and psychological side effects 27,117,118 . These complications could be prevented by saving and banking reproductive organs and tissue before treatment, then re-implanting them afterward 27,28,119,120 .…”

Section: Challenges In Complex Tissue Preservationmentioning

confidence: 99%

The promise of organ and tissue preservation to transform medicine

et al. 2017

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The ability to replace organs and tissues on demand could save or improve millions of lives each year globally and create public health benefits on par with curing cancer. Unmet needs for organ and tissue preservation place enormous logistical limitations on transplantation, regenerative medicine, drug discovery, and a variety of rapidly advancing areas spanning biomedicine. A growing coalition of researchers, clinicians, advocacy organizations, academic institutions, and other stakeholders has assembled to address the unmet need for preservation advances, outlining remaining challenges and identifying areas of underinvestment and untapped opportunities. Meanwhile, recent discoveries provide proofs of principle for breakthroughs in a family of research areas surrounding biopreservation. These developments indicate that a new paradigm, integrating multiple existing preservation approaches and new technologies that have flourished in the past 10 years, could transform preservation research. Capitalizing on these opportunities will require engagement across many research areas and stakeholder groups. A coordinated effort is needed to expedite preservation advances that can transform several areas of medicine and medical science.

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Morphology and function of cryopreserved whole ovine ovaries after heterotopic autotransplantation

Cited by 40 publications

References 52 publications

Cellular proliferation and vascularization in ovine fetal ovaries: effects of undernutrition and selenium in maternal diet

Cellular proliferation and vascularization in ovine fetal ovaries: effects of undernutrition and selenium in maternal diet

Follicle growth and oocyte development after ovary transplantation into back muscle of immune-intact adult castrated male mice

The promise of organ and tissue preservation to transform medicine

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