Follicle growth and oocyte development after ovary transplantation into back muscle of immune-intact adult castrated male mice

Li, Fengyin; Tao, Yong; Zhang, Yunhai; Li, Yunsheng; Fang, Fugui; Liu, Ya; H, Cao; Zhang, Xiaorong; ShiXian, Zhou

doi:10.1530/rep-10-0076

Cited by 21 publications

(16 citation statements)

References 71 publications

(100 reference statements)

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“…E 2 and P 4 are the most representative steroids (Li et al 2010). In this study, the mean serum concentration of E 2 increased significantly in the autografted EPO-treated group.…”

Section: Discussionsupporting

confidence: 55%

“…We also chose the back muscle site, as more rapid revascularization occurs in an i.m. transplantation site than in other graft sites (Israely et al 2003, Soleimani et al 2008, Dath et al 2010, Li et al 2010, Commin et al 2012.…”

Section: Discussionmentioning

confidence: 97%

“…Endocrinology of ovary-grafted animals helps us to understand follicular and oocyte development and also the return of ovarian function (Callejo et al 2002, Li et al 2010). E 2 and P 4 are the most representative steroids (Li et al 2010).…”

Section: Discussionmentioning

confidence: 99%

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Effects of erythropoietin on ischemia, follicular survival, and ovarian function in ovarian grafts

Mahmoodi¹,

Mehranjani²,

Shariatzadeh³

et al. 2014

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Ovarian tissue transplantation is performed to preserve fertility in patients undergoing chemotherapy and radiotherapy. However, ischemia/reperfusion (IR) injury and free radical production occurring during the revascularization of the transplanted tissue are the major limitations of this procedure. The aim of this study was to investigate the effect of erythropoietin (EPO) as an antioxidant on oxidative stress and ovary survival following transplantation. The Naval Medical Research Institute (NMRI) mice (4-5 weeks old) were divided into three groups (six mice per group): control; autograftCsaline, and autograftCEPO (500 IU/kg i.p.). After 28 days, ovary compartments were estimated stereologically. DNA fragmentation and plasma malondialdehyde (MDA), progesterone, and estradiol (E 2 ) concentrations were also evaluated. The results were analyzed using one-way ANOVA and Tukey's test, and the means were significantly different at P!0.05. The mean total volume of ovary, cortex, and medulla and the number of follicles increased significantly in the autograftCEPO group (P!0.01). Apoptosis rate in the autograftCEPO group was lower than that in the autograftCsaline group. The concentration of MDA decreased significantly in the autografted EPO-treated group than in the autografted saline-treated group (P!0.01). The concentration of E 2 increased significantly in the autograftCEPO group than in the autograftCsaline group (P!0.01). EPO reduced IR injury, increasing follicle survival and function in grafted ovaries. Free Persian abstractA Persian (Farsi) translation of the abstract is freely available online at

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“…E 2 and P 4 are the most representative steroids (Li et al 2010). In this study, the mean serum concentration of E 2 increased significantly in the autografted EPO-treated group.…”

Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Effects of erythropoietin on ischemia, follicular survival, and ovarian function in ovarian grafts

Mahmoodi¹,

Mehranjani²,

Shariatzadeh³

et al. 2014

Reproduction

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“…Ovariectomy was carried out through bilateral incisions on each side of the spinal column in the dorsal body wall. A small incision (0.5 cm) was made along the right and left gluteus superficialis muscle fibres, and the excised ovaries were autografted immediately into the gluteal muscles as the transplantation site as multiple reports have documented the effect of intramuscular transplantation on increasing follicular development and survival rate and fast vascularization (Abir et al, 2011;Dath et al, 2010;Friedman et al, 2012;Li et al, 2010;Soleimani et al, 2008. In addition, Israely et al (2003) compared the efficacy of two transplantation sites (subcutaneous and intramuscular), which found that intramuscular grafts recovered and resembled normal ovaries; however, most of the subcutaneous grafts remained impaired and necrotic (Israely et al, 2003).…”

Section: Ovarian Autotransplantation and N-acetylcysteine Treatmentmentioning

confidence: 99%

N-acetylcysteine improves function and follicular survival in mice ovarian grafts through inhibition of oxidative stress

Mahmoodi

Mehranjani

Shariatzadeh

et al. 2015

Reproductive BioMedicine Online

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The effect of N-acetylcysteine (NAC) on mouse ovary heterotopic autotransplantation was investigated. Mice (age 4-5 weeks) were divided into the following groups: control; autograft plus NAC (150 mg/kg daily intraperitoneal injection) and autograft plus saline (n = 6 per group). Groups were treated from 1 day before until 7 days after transplantation. After 28 days, ovary compartments were estimated stereologically. Plasma malondialdehyde, progesterone, oestradiol concentrations and the percentage of apoptotic follicles were measured to evaluate the rate of oxidative stress and ovarian graft function. The mean total volume of ovary, cortex and the number of follicles was significantly higher (all P < 0.001) in the autografts plus NAC group compared with the saline group. In the autografts plus NAC group, the mean percentage of apoptotic follicles (P < 0.001) and malondialdehyde concentration (P < 0.001 day 7; P < 0.05 day 28) were significantly lower, whereas oestradiol concentration was significantly higher (P < 0.05) compared with the saline group. Although NAC cannot compensate the above parameters to the control level, it considerably improves follicular survival and development and also the structure and function of transplanted ovaries, through reducing oxidative stress and apoptosis.

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“…In all treatment groups at day 14 after transplantation there was an increase in the number of functional corpora lutea, but this was significant just in the group treated with NAC150 (p=0.02 . Li et al 28 also describe an elevation of estradiol levels studying follicular growth and oocyte development after transplantation in muscle of spayed male mice.…”

Section: Discussionmentioning

confidence: 99%

N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats

et al. 2014

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PURPOSE:To evaluate morphological and functional aspects of the ovarian graft in transplanted rats treated with NAC. METHODS:Female Wistar rats, virgin, 3 to 4 months old, weighing 200-250 grams were used in experiments. The rats have been kept in proper sanitary conditions, receiving food and water ad libitum. Five groups (n=10, each) were constituted: 4 groups treated subcutaneously with NAC, at doses of 150, 300, 600 and 1200 mg/kg (NAC150, NAC300, NAC600 and NAC1200, respectively), one hour of before the ovarian transplantation and control group (GTx) -treated with physiological solution and submitted to ovarian transplantation. The rats were anesthetized and submitted to autologous left ovarian transplantation, without anastomosis in retroperitoneum, and contralateral oophorectomy. During follow-up of 4 or 15 days, the estrous cycle was evaluated by vaginal smears to determine cycle regularity. At the end of 4 th or 15 th days, rats were re-anesthetized and blood and graft were obtained to estradiol analysis and morphological assessment. Data were analysed by One Way Analysis of Variance (ANOVA) or ANOVA on ranks complemented by Student-Newman-Keuls test. RESULTS:At 4 th day, viable follicles in the graft did not altered by NAC treatments. The NAC300 and NAC600 groups showed increasing in follicle atresia (p=0.012) compared to GTx and NAC1200 group. At 15 th day, 50% of GTx, NAC150, and NAC300 rats showed regular oestrous cycle; 83% of NAC600 and 100% of NAC1200 rats returned to regular cycle. NAC1200 group showed increasing in primordial follicle compared to GTx, NAC150 or NAC300 (p=0.011). NAC did not interfere in estradiol levels after 4 or 15 days of transplantation. CONCLUSION:In autologous ovarian transplantation, high dose of NAC promotes graft viability with recovery of estrous cycle.

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Follicle growth and oocyte development after ovary transplantation into back muscle of immune-intact adult castrated male mice

Cited by 21 publications

References 71 publications

Effects of erythropoietin on ischemia, follicular survival, and ovarian function in ovarian grafts

Effects of erythropoietin on ischemia, follicular survival, and ovarian function in ovarian grafts

N-acetylcysteine improves function and follicular survival in mice ovarian grafts through inhibition of oxidative stress

N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats

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