Morphology and density of glandular trichomes of Ocimum campechianum and Ruellia nudiflora in contrasting light environments: A scanning electron microscopy study

Martínez-Natarén, Daniela A.; Villalobos-Perera, Pedro A.; Munguía-Rosas, Miguel A.

doi:10.1016/j.flora.2018.08.011

Cited by 16 publications

(3 citation statements)

References 39 publications

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“…Drought stress slightly enhanced the glandular hair densities of the basil species and the TPC of O. basilicum 'Genovese'. Other scholars, however, have stated that trichome density is mainly determined by ontogenesis and species [51,52]. We observed that water supply can also significantly influence the glandular hair density.…”

Section: Discussionmentioning

confidence: 45%

Influence of Drought Stress on Growth and Essential Oil Yield of Ocimum Species

Musie

Radácsi

2022

Horticulturae

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A pot experiment was conducted to assess the effect of drought stress on growth and secondary compound accumulation of Ocimum species, in a semi-controlled greenhouse during the year 2020. The experiment was performed as a factorial that was based on a randomized complete design with three species of basil namely O. basilicum ‘Genovese’, O. x africanum, and O. americanum, and three levels of water supply (70, 50, and 30% soil water capacity-SWC) that were replicated twice. The results revealed that severe drought conditions (30% SWC) significantly lowered the relative water content and, as a consequence, plants were shorter, narrower in the canopy, and produced smaller leaves which, in turn, resulted in a 50% fresh and dry herb yield loss. Furthermore, severe drought stress negatively affected the essential oil content (except O. x africanum where no change was seen), essential oil yield, and the antioxidant capacity. However, slight enhancements of glandular hair density were observed in the drought treatment than in the control. Regardless of the soil water capacity variation, the major compound of the essential oil and total polyphenol content remained unchanged. Besides drought, morphological and chemical variations were also detected among the Ocimum species. Sweet basil cultivar Genovese produced the maximum biomass (344.7 g/plant) whereas O. x africanum accumulated higher essential oil (2.79%). The major compounds that were identified were linalool in O. basilicum; 1, 8-cineole, and camphor in O. x africanum; and O. americanum had more neral and geranial. In conclusion, a higher water supply is recommended for higher biomass and essential oil yield production in the tested basil species.

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Section: Discussionmentioning

confidence: 45%

Influence of Drought Stress on Growth and Essential Oil Yield of Ocimum Species

Musie

Radácsi

2022

Horticulturae

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“…Here, we confirmed that environmental factors, including light conditions, could flexibly control GT density. A previous study showed that two light conditions (full solar radiation vs. shade) had no impact on GT density in a medicinal plant, Ocimum campechianum [ 22 ]. In cultivated tomato plants, a density of type VI leaf GTs increased under high light conditions (approximately 300 µmol/m 2 /s) [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Single Wavelengths of LED Light Supplement Promote the Biosynthesis of Major Cyclic Monoterpenes in Japanese Mint

Ueda

Murata

Yokawa

2021

Plants

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Environmental light conditions influence the biosynthesis of monoterpenes in the mint plant. Cyclic terpenes, such as menthol, menthone, pulegone, and menthofuran, are major odor components synthesized in mint leaves. However, it is unclear how light for cultivation affects the contents of these compounds. Artificial lighting using light-emitting diodes (LEDs) for plant cultivation has the advantage of preferential wavelength control. Here, we monitored monoterpene contents in hydroponically cultivated Japanese mint leaves under blue, red, or far-red wavelengths of LED light supplements. Volatile cyclic monoterpenes, pulegone, menthone, menthol, and menthofuran were quantified using the head-space solid phase microextraction method. As a result, all light wavelengths promoted the biosynthesis of the compounds. Remarkably, two weeks of blue-light supplement increased all compounds: pulegone (362% increase compared to the control), menthofuran (285%), menthone (223%), and menthol (389%). Red light slightly promoted pulegone (256%), menthofuran (178%), and menthol (197%). Interestingly, the accumulation of menthone (229%) or menthofuran (339%) was observed with far-red light treatment. The quantification of glandular trichomes density revealed that no increase under light supplement was confirmed. Blue light treatment even suppressed the glandular trichome formation. No promotion of photosynthesis was observed by pulse-amplitude-modulation (PAM) fluorometry. The present result indicates that light supplements directly promoted the biosynthetic pathways of cyclic monoterpenes.

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“…The total number of C. pulegium glandular trichomes (P + C1 + C2) per leaf unit area on the adaxial leaf side did not vary with the treatment, but on the abaxial side the trichome density was significantly increased by glucose (433.3 ± 17.0 mm −2 ), compared to sucrose (335.9 ± 14.6 mm −2 ). Higher density of glandular trichomes on the abaxial relative to the adaxial leaf surface has been reported in a number of aromatic plant species [44][45][46]. Tozin et al [44] reported an increase in the density of glandular trichomes on the adaxial leaf surface of Ocimum gratissimum upon herbivory.…”

Section: Effect Of Carbon Source On Glandular Trichome Characteristicsmentioning

confidence: 91%

Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source

Stojičić

Tošić

Stojanović

et al. 2022

Plants

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Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant–plant and plant–insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography–mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.

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Morphology and density of glandular trichomes of Ocimum campechianum and Ruellia nudiflora in contrasting light environments: A scanning electron microscopy study

Cited by 16 publications

References 39 publications

Influence of Drought Stress on Growth and Essential Oil Yield of Ocimum Species

Influence of Drought Stress on Growth and Essential Oil Yield of Ocimum Species

Single Wavelengths of LED Light Supplement Promote the Biosynthesis of Major Cyclic Monoterpenes in Japanese Mint

Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source

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