Morphology and adhesion of biomolecules on silicon based surfaces

Bhushan, Bharat; Tokachichu, Dharma R.; Keener, Matthew T.; Lee, Stephen C.

doi:10.1016/j.actbio.2005.01.002

Cited by 72 publications

(89 citation statements)

References 26 publications

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“…Biofunctionalization (i.e. streptavidin-biotin system) of SiO 2 surfaces is commonly required to enhance the binding of biomolecules by modifying the SiO 2 surface with components that will bind biomolecules [30,31].…”

Section: Substrates Influence Dynamic Immobilization Properties Of Dnmentioning

confidence: 99%

Dynamic analysis of DNA nanoparticle immobilization to model biomaterial substrates using combinatorial spectroscopic ellipsometry and quartz crystal microbalance with dissipation

et al. 2014

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Section: Substrates Influence Dynamic Immobilization Properties Of Dnmentioning

confidence: 99%

Dynamic analysis of DNA nanoparticle immobilization to model biomaterial substrates using combinatorial spectroscopic ellipsometry and quartz crystal microbalance with dissipation

et al. 2014

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“…Silicon/SiO 2 substrate cleaning. Semiconductorgrade silicon wafers (identical to those used in Bhushan et al 2005Bhushan et al , 2006aLee et al 2005;Eteshola et al 2008) were purchased from Wafer World, Inc. (West Palm Beach, FL). Diced silicon wafers were prepared as described previously , with one modification.…”

Section: Substratesmentioning

confidence: 99%

Nanoscale adhesion, friction and wear studies of biomolecules on silane polymer-coated silica and alumina-based surfaces

Bhushan

Kwak

Gupta

et al. 2008

J. R. Soc. Interface.

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Proteins on biomicroelectromechanical systems (BioMEMS) confer specific molecular functionalities. In planar FET sensors (field-effect transistors, a class of devices whose protein-sensing capabilities we demonstrated in physiological buffers), interfacial proteins are analyte receptors, determining sensor molecular recognition specificity. Receptors are bound to the FET through a polymeric interface, and gross disruption of interfaces that removes a large percentage of receptors or inactivates large fractions of them diminishes sensor sensitivity. Sensitivity is also determined by the distance between the bound analyte and the semiconductor. Consequently, differential properties of surface polymers are design parameters for FET sensors. We compare thickness, surface roughness, adhesion, friction and wear properties of silane polymer layers bound to oxides (SiO 2 and Al 2 O 3 , as on AlGaN HFETs). We compare those properties of the film-substrate pairs after an additional deposition of biotin and streptavidin. Adhesion between protein and device and interfacial friction properties affect FET reliability because these parameters affect wear resistance of interfaces to abrasive insult in vivo. Adhesion/friction determines the extent of stickage between the interface and tissue and interfacial resistance to mechanical damage. We document systematic, consistent differences in thickness and wear resistance of silane films that can be correlated with film chemistry and deposition procedures, providing guidance for rational interfacial design for planar AlGaN HFET sensors.

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“…AFM is a useful technique, not only because it can image nanoscale features, but also because it can operate in many different modes and under many different conditions. For instance, in addition to the standard height mapping [2,3], AFM can perform: phase mapping, measuring viscoelastic differences on a surface; friction mapping, measuring friction variation on a surface; and adhesion mapping, measuring adhesive variation on a surface. Taken together, AFM can provide comprehensive surface characterization.…”

Section: Atomic Force Microscopy Characterization Of the Block Copolymentioning

confidence: 99%

“…Biosensors need to detect specific analytes from blood or other body fluids, and indiscriminate protein adsorption can affect the sensitivity and functionality of the sensors [1][2][3][4]. Degradable and non-degradable biomaterials are also affected by the proteins adsorbed on their surfaces.…”

Section: Introductionmentioning

confidence: 99%

Designing nanostructured block copolymer surfaces to control protein adhesion

Schricker

Palacio

Bhushan

2012

Phil. Trans. R. Soc. A.

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The profile and conformation of proteins that are adsorbed onto a polymeric biomaterial surface have a profound effect on its in vivo performance. Cells and tissue recognize the protein layer rather than directly interact with the surface. The chemistry and morphology of a polymer surface will govern the protein behaviour. So, by controlling the polymer surface, the biocompatibility can be regulated. Nanoscale surface features are known to affect the protein behaviour, and in this overview the nanostructure of self-assembled block copolymers will be harnessed to control protein behaviour. The nanostructure of a block copolymer can be controlled by manipulating the chemistry and arrangement of the blocks. Random, A-B and A-B-A block copolymers composed of methyl methacrylate copolymerized with either acrylic acid or 2-hydroxyethyl methacrylate will be explored. Using atomic force microscopy (AFM), the surface morphology of these block copolymers will be characterized. Further, AFM tips functionalized with proteins will measure the adhesion of that particular protein to polymer surfaces. In this manner, the influence of block copolymer morphology on protein adhesion can be measured. AFM tips functionalized with antibodies to fibronectin will determine how the surfaces will affect the conformation of fibronectin, an important parameter in evaluating surface biocompatibility.

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Morphology and adhesion of biomolecules on silicon based surfaces

Cited by 72 publications

References 26 publications

Dynamic analysis of DNA nanoparticle immobilization to model biomaterial substrates using combinatorial spectroscopic ellipsometry and quartz crystal microbalance with dissipation

Dynamic analysis of DNA nanoparticle immobilization to model biomaterial substrates using combinatorial spectroscopic ellipsometry and quartz crystal microbalance with dissipation

Nanoscale adhesion, friction and wear studies of biomolecules on silane polymer-coated silica and alumina-based surfaces

Designing nanostructured block copolymer surfaces to control protein adhesion

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