Dynamic analysis of DNA nanoparticle immobilization to model biomaterial substrates using combinatorial spectroscopic ellipsometry and quartz crystal microbalance with dissipation

Abstract

Dynamic analysis of DNA nanoparticle immobilization to model biomaterial substrates using combinatorial spectroscopic ellipsometry and quartz crystal microbalance with dissipation" (2014).

Investigation of Bovine Serum Albumin (BSA) Attachment onto Self-Assembled Monolayers (SAMs) Using Combinatorial Quartz Crystal Microbalance with Dissipation (QCM-D) and Spectroscopic Ellipsometry (SE)

“…n-Alkanethiols attach to Au surfaces by chemisorption at a thiol group to form close-packed SAMs, leaving the other end of function groups (–CH 3, –OH, –COOH, and –NH 2 ) available to bind proteins [ 26 ]. The Au substrate was chosen because it is biocompatible and can be easily modified with SAM attachment by forming stable metal-sulfur bonds [ 18 , 27 , 28 ].…”

“…All solutions were passed through the liquid cell using a peristaltic pump (Ismatec IPC-N 4) (Biolin Scientific) attached to Tygon tubing (Fisher Scientific) and a 90 ° flow path 3-port valve (Hamilton) at a flow rate of 0.1 mL/min. DI H 2 O was pumped into the liquid chamber and both instruments were allowed to equilibrate for approximately 2 hr to develop a baseline reading [ 27 ]. After stabilization with DI H 2 O, BSA solution was introduced into the flow cell for 30 min and then the flow was stopped to allow the BSA molecules to adsorb to the SAM surface for approximately 70 min.…”

“…After stabilization with DI H 2 O, BSA solution was introduced into the flow cell for 30 min and then the flow was stopped to allow the BSA molecules to adsorb to the SAM surface for approximately 70 min. DI H 2 O was then pumped into the liquid cell to remove any passively-attached BSA molecules [ 27 ]. Thickness, mass and porosity changes in SE and QCM-D induced by BSA attachment were monitored continuously, and data acquisition was performed using CompleteEASE (J.A.…”

“…There are a limited number of studies of BSA adsorption on biofunctional and environmentally-relevant surfaces such as self-assembled monolayers (SAMs). A recent study analyzing DNA nanoparticle and fetal bovine serum (FBS) protein attachment to model biomaterial substrates was one of the first to evaluate protein adsorption using QCM-D/SE [ 15 , 23 ].…”

The retention of liquid by columnar nanostructured surfaces during quartz crystal microbalance measurements and the effects of adsorption thereon

“…Adsorption and desorption processes [1][2][3], surface chemical reactions [4,5], and reorganization of chemical species [6,7] and the kinetics thereof are widely studied at the solid-liquid interface. Thus far, such processes are commonly studied on flat surfaces.…”