Morphology analysis of<i>Escherichia coli</i>treated with nonthermal plasma

Guo, Jiansheng; Li, Z.; Huang, Kuan-Chun; Li, Y.; Wang, J.

doi:10.1111/jam.13335

Cited by 14 publications

(3 citation statements)

References 33 publications

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“…This phase is dominated by an erosion process through photodesorption by UV photons or through etching by active species [5, 7, 10, 33]. During this phase, RONS attack the unsaturated fatty acids which are highly present in Gram negative cell membrane [31, 52]; oxygen atoms can also diffuse into the cell causing oxidative stress possibly leading to antimicrobial effect [53]. …”

Section: Discussionmentioning

confidence: 99%

Innovative non-thermal plasma disinfection process inside sealed bags: Assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms

et al. 2017

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In this work, we developed a device capable to generate a non-thermal plasma discharge inside a sealed bag. The aim of this study was to assess the effectiveness of the oxygen, nitrogen and argon plasma sterilization on Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis spores according to the NF EN 556 Norm. Moreover the bag integrity which is a critical key to maintain the sterile state of items after the end of the process was verified by Fourier Transform Infrared (FTIR) and X-ray Photoelectron Spectrometry (XPS) analyses. After plasma treatments, the bacterial counting showed a 6 log reduction of P. aeruginosa and S. aureus in 45 min and 120 min respectively whatever the gas used and a 4 log reduction of B. subtilis spores in 120 min with only oxygen plasma. These results were confirmed by Scanning Electron Microscopy (SEM) observations showing altered bacteria or spores and numerous debris. Taking into account the studied microorganisms, the oxygen plasma treatment showed the highest efficiency. FTIR and XPS analyses showed that this treatment induced no significant modification of the bags. To conclude this non-thermal plasma sterilization technique could be an opportunity to sterilize heat and chemical-sensitive medical devices and to preserve their sterile state after the end of the process.

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Section: Discussionmentioning

confidence: 99%

Innovative non-thermal plasma disinfection process inside sealed bags: Assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms

et al. 2017

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“…It is important that the presence of oxygen in the medium is more significant than the type of carrier gas [ 12 ]. The mechanism of this antibacterial effect is considered to be a toxic effect on the cell wall of microorganisms [ 13 ] as well as on the inactivation of key oxygenases of bacteria [ 14 ].…”

Section: Cold Plasma As a Means Of Disinfectionmentioning

confidence: 99%

Cold Argon Athmospheric Plasma for Biomedicine: Biological Effects, Applications and Possibilities

et al. 2022

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Currently, plasma medicine is a synthetic direction that unites the efforts of specialists of various profiles. For the successful formation of plasma medicine, it is necessary to solve a large complex of problems, including creating equipment for generating cold plasma, revealing the biological effects of this effect, as well as identifying and justifying the most promising areas of its application. It is known that these biological effects include antibacterial and antiviral activity, the ability to stimulate hemocoagulation, pro-regenerative properties, etc. The possibility of using the factor in tissue engineering and implantology is also shown. Based on this, the purpose of this review was to form a unified understanding of the biological effects and biomedical applications of argon cold plasma. The review shows that cold plasma, like any other physical and chemical factors, has dose dependence, and the variable parameter in this case is the exposure of its application. One of the significant characteristics determining the specificity of the cold plasma effect is the carrier gas selection. This gas carrier is not just an ionized medium but modulates the response of biosystems to it. Finally, the perception of cold plasma by cellular structures can be carried out by activating a special molecular biosensor, the functioning of which significantly depends on the parameters of the medium (in the field of plasma generation and the cell itself). Further research in this area can open up new prospects for the effective use of cold plasma.

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“…The ability of OH radicals to induce biological damage is often exploited in the medical fields as therapeutic tools. Among these, reactive oxygen species (ROS) generated by non-thermal atmospheric-pressure plasma are an important means of inactivating microbial pathogens [5,6]. More recently, the efficiency of the cold argon plasma jet and dielectric barrier discharge to inactivate mycobacteria were reported [7,8].…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Mycobacteria by Radicals from Non-Thermal Plasma Jet

Lee¹,

Subhadra²,

Choi³

et al. 2019

Journal of Microbiology and Biotechnology

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Mycobacterial cell walls comprise thick and diverse lipids and glycolipids that act as a permeability barrier to antibiotics or other chemical agents. The use of OH radicals from a non-thermal plasma jet (NTPJ) for the inactivation of mycobacteria in aqueous solution was adopted as a novel approach. Addition of water vapor in a nitrogen plasma jet generated OH radicals, which converted to hydrogen peroxide (H 2 O 2) that inactivated non-pathogenic Mycobacterium smegmatis and pathogenic Mycobacterium tuberculosis H37Rv. A stable plasma plume was obtained from a nitrogen plasma jet with 1.91 W of power, killing Escherichia coli and mycobacteria effectively, whereas addition of catalase decreased the effects of the former. Mycobacteria were more resistant than E. coli to NTPJ treatment. Plasma treatment enhanced intracellular ROS production and upregulation of genes related to ROS stress responses (thiolrelated oxidoreductases, such as SseA and DoxX, and ferric uptake regulator furA). Morphological changes of M. smegmatis and M. tuberculosis H37Rv were observed after 5 min treatment with N 2 +H 2 O plasma, but not of pre-incubated sample with catalase. This finding indicates that the bactericidal efficacy of NTPJ is related to the toxicity of OH and H 2 O 2 radicals in cells. Therefore, our study suggests that NTPJ treatment may effectively control pulmonary infections caused by M. tuberculosis and nontuberculous mycobacteria (NTM) such as M. avium or M. abscessus in water.

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Morphology analysis ofEscherichia colitreated with nonthermal plasma

Abstract: A clear understanding of the mechanisms underlying nonthermal plasma's inactivation of micro-organism is essential for the practical applications of nonthermal plasma techniques.

Cited by 14 publications

References 33 publications

Innovative non-thermal plasma disinfection process inside sealed bags: Assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms

Innovative non-thermal plasma disinfection process inside sealed bags: Assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms

Cold Argon Athmospheric Plasma for Biomedicine: Biological Effects, Applications and Possibilities

Inactivation of Mycobacteria by Radicals from Non-Thermal Plasma Jet

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