Morphological, Ultrastructural, and Molecular Characterization of Sarcocystis tenella from Sheep in Qena Governorate, Upper Egypt

Hussein, Nermean M.; Hassan, Amal A.; Ella, Osama H. Abd

doi:10.21608/eajbse.2018.14456

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…Most researchers observed that the predominant species in sheep was thick walled sarcocyst (S. tenella). Hussein et al (2018) examined 104 sheep from different organ samples (esophagus and heart muscles) identifying it as Sarcocystis tenella also study of Arshad et al (2007) recorded that the prevalence of Sarcocystis tenella was found in 100 % of the all organs. Nedjari (2002) demonstrated that in Algeria, S. tenella predominated 60.63% to S. arieticanis 39.36 %.…”

Section: Resultsmentioning

confidence: 97%

Histopathological diagnosis for Sarcocystis spp. in slaughtered sheep and goats in Misan governorate/Iraq

Mohammed

Manal

Dragh

2022

ijhs

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This study aimed to determine and identify Sarcocystis spp. infection in sheep and goats in Misan governorate, Iraq. samples from oesophagus, diaphragm, tongue, and heart muscles were examined histologically for microscopic and macroscopic cyst , 70 out of 90 sheep 77.78 % had microscopic thick walled sarcocysts with mean 53 ×24.72 μm diagnosed as Sarcocystis tenella while 25 out of them 27.78 % had microscopic thin-walled sarcocysts with mean 49.45×33 μm diagnosed Sarcocystis arieticanis , esophagus showed the highest infection rate above all organs examined for thick – walled Sarcocystis while diaphragm was the highest for thin –walled Sarcocystis , the heart was the lowest infected organ for both type as 27.78% and 3.33% respectively . In goats 50 sample examined revealed 20 infected with thick walled sarcocysts only with different in distribution according to organs being high in esophagus 40% and lowest in heart 16% with mean measurement 60.61 × 37.75 μm diagnosed as Sarcocystis capracanis.

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Section: Resultsmentioning

confidence: 97%

Histopathological diagnosis for Sarcocystis spp. in slaughtered sheep and goats in Misan governorate/Iraq

Mohammed

Manal

Dragh

2022

ijhs

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“…Out of the five species herein molecularly identified, S. arieticanis predominantly occurs in sheep [e.g., (33, 47)], S. capracanis in goat [e.g., (33, 48)], S. cruzi in cattle [e.g., (49,50)], S. miescheriana in wild boar [e.g., (51)(52)(53)], and S. tenella also in sheep [e.g., (47,54)]. The role of canids (e.g., dog, jackal, raccoon dog, red fox, and gray wolf) as definitive hosts of these Sarcocystis spp.…”

Section: Discussionmentioning

confidence: 99%

“…capracanis in goat [e.g., ( 33 , 48 )], S. cruzi in cattle [e.g., ( 49 , 50 )], S . miescheriana in wild boar [e.g., ( 51–53 )], and S. tenella also in sheep [e.g., ( 47 , 54 )]. The role of canids (e.g., dog, jackal, raccoon dog, red fox, and gray wolf) as definitive hosts of these Sarcocystis spp.…”

Section: Discussionmentioning

confidence: 99%

Red foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) as potential spreaders of Sarcocystis species

Máca,

Gudiškis,

Butkauskas

et al. 2024

Front. Vet. Sci.

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BackgroundSarcocystis includes a global group of apicomplexan parasites with two-host life cycle frequently circulating in wildlife and domestic hosts, including humans. Two of the most important wild terrestrial carnivores acting as definitive hosts are the red fox and raccoon dog, due to their wide distribution in Europe and usage of wild and farmed animals as prey. This study was conducted to determine the prevalence of Sarcocystis in hunted red foxes and raccoon dogs from nine regions of the Czech Republic and to identify isolated sporocysts by molecular techniques.MethodsApproximately 5 g of the contents of large intestine from 200 animals (197 red foxes and three raccoon dogs) were examined by flotation centrifugation coprological method. Only samples of 50 red foxes and one raccoon dog positive to Sarcocystis spp. were used for the nested PCR (nPCR) method to amplify a fragment or partial sequence on the cox1 gene. Ten species-specific primer pairs for detection of Sarcocystis spp. using farm animals as intermediate hosts were utilized.ResultsIn total, 38.1% of the red foxes and 66.7% of the raccoon dogs were positive to Sarcocystis by light microscopy. The molecular characterization resulted in the identification of five species in the red fox: S. arieticanis, S. capracanis, S. cruzi, S. miescheriana, and S. tenella, while the PCR was negative for the sole raccoon dog. The highest intraspecific variation was found for S. miescheriana, while S. tenella was the most prevalent. Co-infections occurred in the large intestine of the red fox. No zoonotic species were found in our samples.ConclusionThis is the first study where the potential role of the red fox and raccoon dogs as spreaders of Sarcocystis to farm animals in the Czech Republic is shown. The use of species-specific primers provides a fast and easy method for screening multiple samples for a particular Sarcocystis species.

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“…Both species, S. gigantea and S. medusiformis are transmitted through felids and produce macrocysts. S. tenella and S. arieticanis, on the other hand, are transmitted through canids and produce microcysts (1,7,8). Infections from S. tenella, S. gigantea and S. arieticanis are common worldwide, whereas S. medusiformis infections have been exclusively reported in Australia, New Zealand, Iran and Italy (4,9).…”

Section: Introductionmentioning

confidence: 99%

Morphological, ultrastructural, genetic characteristics and remarkably low prevalence of macroscopic Sarcocystis species isolated from sheep and goats in Kurdistan region, Iraq

Nawshirwan,

Heucken,

Piekarek

et al. 2023

Front. Vet. Sci.

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IntroductionSarcocystis is a genus of cyst-forming parasites that infest both humans and livestock. Some parasites cause clinical and subclinical diseases in their hosts, resulting in economic losses.MethodsEsophagus, diaphragm, and skeletal muscle from slaughtered sheep and goats were examined macroscopically, microscopically, and ultrastructurally and subjected to DNA analysis.ResultsWe isolated macrocysts of S. gigantea and of S. caprafelis moulei from naturally infected sheep (Ovis aries) and goats (Capra hircus). The macrocyst wall thickness was 18.9 µm in sheep and 15.3 µm in goats, and consisted of an inner Periodic acid Schiff- (PAS) negative primary wall and an outer glycoconjugates containing i.e. PAS-positive secondary wall. The walls inner surface was compartmentalized and filled with bradyzoites. In S. gigantea the bradyzoites were approximently 12.3 x 2.6 µm in size, while in S. caprafelis moulei they were 13.9 x 4.4 µm. Ultrastructurally, both species have nearly identical morphology: cauliflower-like protrusions with numerous microtubules and often dendritic-like filaments, branching from the primary wall. The 18S rRNA gene in S. gigantea was 85.9% identical to that in S. medusiformis and 80.4% to the S. caprafelis moulei gene. The 28S rRNA gene in S. gigantea was 94.6% identical to that in S. medusiformis and 97.3% to the S. caprafelis moulei.ConclusionThis study is the first to (i) detail the ultrastructure of the macrocyst wall of S. caprafelis moulei, (ii) identify S. medusiformis in Iraqi sheep, and (iii) compare the prevalence of macroscopic Sarcocystis at different time periods within the same region. A positive finding was the reduction of macroscopic sarcocystosis occurrences (0.01% in sheep and 0.02% in goats) compared to our previous data from 1992 (4.1%: sheep, 33.6%: goats).

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Morphological, Ultrastructural, and Molecular Characterization of Sarcocystis tenella from Sheep in Qena Governorate, Upper Egypt

Cited by 4 publications

References 17 publications

Histopathological diagnosis for Sarcocystis spp. in slaughtered sheep and goats in Misan governorate/Iraq

Histopathological diagnosis for Sarcocystis spp. in slaughtered sheep and goats in Misan governorate/Iraq

Red foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) as potential spreaders of Sarcocystis species

Morphological, ultrastructural, genetic characteristics and remarkably low prevalence of macroscopic Sarcocystis species isolated from sheep and goats in Kurdistan region, Iraq

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