Morphological study of the effects of textile-immobilized enzymes on an experimental purulent wound

Abstract

Morphological changes in an experimental purulent wound in a rat model is studied for application of surgical gauze with immobilized enzymes: trypsin, lysozyme, coUitin, or co-immobilized trypsin and lysozyme. Comparison of the times of wound cleansing and healing shows that immobilized enzymes are more effective than native preparations, and the therapeutic effect of gauze with the enzyme complex is higher than that of gauze with individually immobilized enzymes. Morphological studies confirm that immobilized trypsin-lysozyme complex and coilitin are the most efficient in hastening and potentiating reparative processes in a purulent wound.

Covalent immobilization of enzyme on aminated woven poly (lactic acid) via ammonia plasma: evaluation of the optimum immobilization conditions

“…Thus, extensive crosslinking has occurred, reducing the accessibility of the substrate and the catalytic activity of the enzyme. 9 That is, the conditions of 2% GA concentration, which showed approximately 92% of the relative activity, has a slight difference of less than 0.5%, compared with 5% GA. Taking into account both the highest increases of activity and chemical efficiency of treatment, 2% GA concentration was considered to be the optimum condition for immobilization of trypsin.…”

“…The reactivity of GA is affected by pH value, with most GA-mediated crosslinking occurring within a slightly alkaline pH range. 9 In order to determine the effect of pH of the GA solution, change in trypsin activity at different pH values (ranging from pH 7.0 to 11.0) of GA was evaluated. Figure 2(a) shows the relative and recovered activity of immobilized trypsin depending on the pH of GA solution during crosslinking.…”

“…This decrease in activity resulted from the extensive aggregation of the support due to increased GA polymerization during excessive crosslinking times. 9 Moreover, the loss of activity may be attributable to diffusion limitation of the reaction within the amine groups of the enzyme. 45 For any given treatment time, the number of enzymatic amine groups reacting with GA molecules were limited.…”

“…In terms of cleavage reaction, trypsin is very specific. For this reason, trypsin has been used not only as medical material, 9 but also in various textile fields. 10–13 For the further application of trypsin at an effective industrial level, trypsin could be immobilized onto heterogeneous derivatives by a suitable immobilization method.…”