Morphological Properties of a Human Intestinal Spirochete First Isolated from a Patient with Diarrhea in Japan

Tasu, Chollada; Nakamura, Shinichi; Tazawa, Hideki; Hara, Hiromichi; Adachi, Yoshikazu

doi:10.1111/j.1348-0421.2003.tb03459.x

Cited by 7 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Japan, human intestinal spirochetosis has not been well recognized. There have been only two case reports of human intestinal spirochetosis in English language, including the first report by Nakamura et al in 1998, 4,5 and has not been a detailed study.…”

mentioning

confidence: 99%

Human intestinal spirochetosis in Japan; its incidence, clinicopathologic features, and genotypic identification

et al. 2008

View full text Add to dashboard Cite

Human intestinal spirochetosis is a common condition in Western countries, but is not well recognized in Japan. To demonstrate the incidence and clinicopathologic findings of human intestinal spirochetosis in Japan, we retrospectively investigated biopsy, and endoscopically or surgically resected specimens of the large intestine. Among a series of 2556 samples, 11 cases of human intestinal spirochetosis were detected (0.4%). Together with additional nine cases sporadically found, 20 cases of human intestinal spirochetosis were subjected to molecular detection of two strains of spirochetes (Brachyspira aalborgi and Brachyspira pilosicoli) by amplifying species-specific portion of 16S ribosomal RNA and NADH oxydase gene by polymerase chain reaction. B. aalborgi was detected in all cases examined, three of which revealed dual infection of both species. Our results suggest that human intestinal spirochetosis infection is relatively rare, and B. aalborgi is the most prevalent species in Japan. Most of human intestinal spirochetosis were asymptomatic, although symptomatic in exceptional cases. In addition, we emphasize a usefulness of immunostaining with anti-Treponema pallidum and anti-Mycobacterium bovis polyclonal antibodies for detecting the spirochetes.

show abstract

mentioning

confidence: 99%

Human intestinal spirochetosis in Japan; its incidence, clinicopathologic features, and genotypic identification

et al. 2008

View full text Add to dashboard Cite

show abstract

“…After vigorous shaking for 10 sec, 100 µl of the supernatant were spread on plates containing trypticase soy agar (TSA, Difco, Lawrence, KS, U.S.A.) supplemented with 5% defibrinated horse blood and 400 µg of spectinomycin per ml (TSA-400S) at 37°C for 6 days under anaerobic conditions using AnaeroPack (Mitsubishi Gas Chemical Company, Inc., Tokyo, Japan) as described previously [16]. The isolates were identified by genetic and biochemical techniques [9].…”

mentioning

confidence: 99%

Application of Real Time PCR for Diagnosis of Swine Dysentery

Akase

Uchitani

Sohmura

et al. 2009

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Evaluation of a genetic diagnostic technique using real time PCR of Swine Dysentery (SD) was performed using nox primers. Culture, ordinary PCR and real time PCR were compared in this experiment. Sixty-seven specimens from pigs with clinical signs of SD brought to a slaughterhouse in Shibaura, Tokyo, were used. B. hyodysenteriae was isolated from 49 of the pigs, was detected by ordinary PCR in 49 of the pigs and was detected by real time PCR in 54 of the pigs. Furthermore, we were able to determine the numbers of B. hyodysenteriae cells in all positive specimens by real time PCR. The rapid diagnostic technique established in this experiment was useful for detection of B. hyodysenteriae because it was more effective than ordinary PCR and culture.

show abstract

“…), which is a group of intestinal spirochetes has been isolated from pigs, chickens, dogs, and humans, suffering from dysentery and diarrhea, and has been classified into eight species, i.e., B. hyodysenteriae, B. [2,4,6,15,[17][18][19]21]. All species are weakly beta-hemolytic spirochetes, except for B. hyodysenteriae, with beta-hemolysis on blood agar.…”

mentioning

confidence: 99%

The Specific Antibody to Brachyspira aalborgi in Serum Obtained from a Patient with Intestinal Spirochetosis

Abe

Hirane

Yoshizawa

et al. 2006

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Serum obtained from a patient histopathologically diagnosed as intestinal spirochetosis was investigated serodiagnostically by agglutination test. B. aalborgi which is a human intestinal spirochete reacted strongly with the human serum, while B. pilosicoli which has potential pathogenicity to humans reacted with the serum, but as strongly and its titer was different than the other three species. On the other hand, intestinal spirochetes (Matsumoto isolates) were isolated from the biopsy samples of the patient. The morphological, biochemical, and genetic characteristics of the isolates were very similar to those of B. aalborgi. Furthermore, the protein profiles of the Matsumoto isolates were also similar to those of B. aalborgi but were different than those of B. pilosicoli and B. hyodysenteriae. The reaction profiles of the Matsumoto isolates in immunoblotting were relatively similar to those of B. aalborgi except for a 74 kDa band but were different from those of B. pilosicoli and B. hyodysenteriae. Therefore, we identified the Matsumoto isolates as B. aalborgi and diagnosed the patient with a B. aalborgi infection. KEY WORDS: Brachyspira aalborgi, human, intestinal spirochete.

show abstract

Morphological Properties of a Human Intestinal Spirochete First Isolated from a Patient with Diarrhea in Japan

Cited by 7 publications

References 20 publications

Human intestinal spirochetosis in Japan; its incidence, clinicopathologic features, and genotypic identification

Human intestinal spirochetosis in Japan; its incidence, clinicopathologic features, and genotypic identification

Application of Real Time PCR for Diagnosis of Swine Dysentery

The Specific Antibody to Brachyspira aalborgi in Serum Obtained from a Patient with Intestinal Spirochetosis

Contact Info

Product

Resources

About