Morphological organization of neuropile glial cells in the central nervous system of the medicinal leech (Hirudo medicinalis)

Riehl, B.; Schlue, Wolf-Rüdiger

doi:10.1016/s0040-8166(98)80066-9

Cited by 10 publications

(7 citation statements)

References 37 publications

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“…Accordingly, GFAP-like immunoreactivity has been shown in enteric glia (Jessen and Mirsky, 1980), Schwann cells of unmyelinated peripheral nerve fibers (Yen and Fields, 1981;Dahl et al, 1982), lens epithelium (Hatfield et al, 1984), and Kupffer cells in the liver (Gard et al, 1985), among others. Filaments of morphological structure similar to the intermediate glial filament have been reported in the glial cells of many mammals, birds, reptiles, fishes (Dahl et al, 1985), and in some invertebrates such as annelids and mollusks (see Radojcic and Pentreath, 1979;Cardone and Roots, 1990;Riehl and Schlue, 1998;Dos Santos et al, 2002).…”

Section: Introductionmentioning

confidence: 83%

“…Because of the main role that has been attributed to GFAP in astrocytes, as a cytoskeleton stabilizer (see Rutka et al, 1997), and to the presence of this protein in few invertebrates (see Radojcic and Pentreath, 1979;Cardone and Roots, 1990, Riehl and Schlue, 1998, Dos Santos et al, 2002, we wanted to search for the presence of GFAP-like proteins in crustaceans.…”

Section: Introductionmentioning

confidence: 99%

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Glial fibrillary acidic protein (GFAP)‐like immunoreactivity in the visual system of the crab Ucides cordatus (Crustacea, Decapoda)

Silva¹,

Corrêa²,

Tortelote³

et al. 2004

Biology of the Cell

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Glial fibrillary acidic protein (GFAP) is the main intermediate filament protein used as a marker for the identification of astrocytes in the central nervous system of vertebrates. Analogous filaments have been observed in the glial cells of many mollusks and annelids but not in crustaceans. The present study was carried out to identify by light microscopy immunohistochemistry, immunoelectronmicroscopy and immunoblotting, GFAP-like positive structures in the visual system of the crab Ucides cordatus as additional information to help detect and classify glial cells in crustaceans. Conventional electron microscopy, light microscopy of semithin sections and fluorescence light microscopy were also employed to characterize cells and tissues morphology. Our results indicated the presence of GFAP-like positive cell processes and cell bodies in the retina and adjoining optic lobe. The labeling pattern on the reactive profiles was continuous and very well defined, differing considerably from what has been previously reported in the central nervous system of some mollusks, where a diffuse spotted fluorescence pattern of labeling was observed. We suggest that this glial filament protein may be conserved in the evolution of the invertebrate nervous systems and that it may be used as a label for some types of glial cells in the crab.

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Section: Introductionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Glial fibrillary acidic protein (GFAP)‐like immunoreactivity in the visual system of the crab Ucides cordatus (Crustacea, Decapoda)

Silva¹,

Corrêa²,

Tortelote³

et al. 2004

Biology of the Cell

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“…NFs have not been observed in crustaceans using conventional electron microscopy, and IFs in general have not been observed in crustaceans and other arthropods by immunocytochemistry. However, numerous studies in lower vertebrates and invertebrates have revealed the presence of NF proteins similar to mammalian NF subunits (Philips et al 1983;Viancour et al 1987;Leapman et al 1997;Karabinos et al 2001) and IF like glial fibrillary acidic protein (GFAP) (Cardone and Roots 1990;Kumar et al 1996;Riehl and Schlue 1998;dos Santos et al 2002). Particularly interesting for crustacean neurobiologists was the study conducted by Weaver and Viancour (1991), representing a very thorough search for mammalian NF proteins in crayfish axons: following labeling with the commercially available anti-NF-M monoclonal antibody (NN18-clone), it revealed a polypeptide located on microtubule side-arms.…”

Section: Introductionmentioning

confidence: 99%

Identification of a neurofilament-like protein in the protocerebral tract of the crab Ucides cordatus

et al. 2004

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Neurofilaments (NFs) have not been observed in crustaceans using conventional electron microscopy, and intermediate filaments have never been described in crustaceans and other arthropods by immunocytochemistry. Since polypeptides, labeled by the NN18-clone antibody, were revealed on microtubule side-arms of crayfish, we have tested, in this study, whether proteins similar to mammalian NFs are present in the protocerebral tract (PCT) of the crab Ucides cordatus. We used immunohistochemistry for light microscopy with monoclonal antibodies against three different NF subunits, high (NF-H), medium (NF-M), and light (NF-L). Labeling was observed with the NN18-clone, which recognizes NF-M. In order to confirm the results obtained with the immunohistochemical reactions, Western blotting, using the three primary antibodies, was performed and the presence of NF-M was confirmed. The NN18-clone monoclonal antibody recognized a protein of approximately 160 kDa, similar to the mammalian NF-M protein, but NF-L and NF-H were not recognized. Conventional transmission electron microscopy was used to observe the ultrastructural components of the axons and immunoelectron microscopy was used to show the distribution of the NF-M-like polypeptides along cytoskeletal elements of the PCT. Our results agree with previous studies on crustacean NF proteins that have reported negative immunoreactions against NF-H and NF-L subunits and positive immunoreactions against the mammalian NF-M subunit. However, the protein previously referred to as P600 and recognized by the NN18-clone, has a very high molecular weight, thus, being different from mammalian NF-M subunit and from the protein revealed now in our study.

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“…Other markers of vertebrate glial cells have not often been tested in invertebrates, although a GFAP-like protein has been shown in some neuropils in annelids (Riehl and Schlue, 1998) and mollusks (Cardone and Roots, 1990;dos Santos et al, 2002). In calanoid copepods, axon wrappings may be formed by concentric and continuous layers of myelin-like laminae, but the cell type that forms it could not be determined (Weatherby et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Binding of an antibody against a noncompact myelin protein to presumptive glial cells in the visual system of the crab Ucides cordatus

Silva

Bressan

Cavalcante

et al. 2003

Glia

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Glial cells, in both vertebrate and invertebrate nervous systems, provide an essential environment for developmental, supportive, and physiological functions. However, information on glial cells themselves and on glial cell markers, with the exception of those of Drosophila and other insects, is not abundant in invertebrate organisms. A common ultrastructural feature of invertebrate nervous systems is that layers of glial cell cytoplasm-rich processes ensheath axons and neuronal and glial somata. In the present study, we have examined the binding of a monoclonal antibody to 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) in the compound eye and optic lobe of the crab Ucides cordatus using both light and electron microscopy. CNPase is a noncompact myelin protein that is a phenotypic marker of oligodendroglial and Schwann cells, is apparently involved in the ensheathment step prior to myelin compaction, and is also expressed by the potentially myelinating olfactory ensheathing glia. CNPase has raised much interest, first by virtue of its unusual enzymatic activity and more recently by its membrane-skeletal features and possible involvement in migration or expansion of membranes. We have found CNPase-like immunoreactivity in most cells of the compound eye basement membrane and both in optic cartridges of the synaptic layer and cells of the outer sublayer of the lamina ganglionaris. The results suggest that in the crab visual system some, but not all, glial cells, including some adaxonal glia, may express the noncompact myelin protein CNPase or a related protein.

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Morphological organization of neuropile glial cells in the central nervous system of the medicinal leech (Hirudo medicinalis)

Cited by 10 publications

References 37 publications

Glial fibrillary acidic protein (GFAP)‐like immunoreactivity in the visual system of the crab Ucides cordatus (Crustacea, Decapoda)

Glial fibrillary acidic protein (GFAP)‐like immunoreactivity in the visual system of the crab Ucides cordatus (Crustacea, Decapoda)

Identification of a neurofilament-like protein in the protocerebral tract of the crab Ucides cordatus

Binding of an antibody against a noncompact myelin protein to presumptive glial cells in the visual system of the crab Ucides cordatus

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