Glial fibrillary acidic protein (GFAP)‐like immunoreactivity in the visual system of the crab <i>Ucides cordatus</i> (Crustacea, Decapoda)

Silva, Simone Florim da; Corrêa, Clynton Lourenço; Tortelote, Giovane G.; Einicker‐Lamas, Marcelo; Martínez, Ana Maria Blanco; Allodi, Silvana

doi:10.1016/j.biolcel.2004.06.005

Cited by 13 publications

(7 citation statements)

References 41 publications

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“…Again this experiment was confirmed by Western blotting [Fig. (B)], which showed a band at ∼45 kDa, therefore, near the molecular weight of the band described for another invertebrate species, the crab Ucides cordatus (∼48 kDa; Silva et al ) and close to the molecular weight found in mammals (∼48 to ∼50 kDa; Yen and Fields, ; Eng, ).…”

Section: Resultssupporting

confidence: 67%

3‐acetylpyridine‐induced degeneration in the adult ascidian neural complex: Reactive and regenerative changes in glia and blood cells

Medina

Abreu

Cavalcante

et al. 2014

Developmental Neurobiology

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Ascidians are interesting neurobiological models because of their evolutionary position as a sister-group of vertebrates and the high regenerative capacity of their central nervous system (CNS). We investigated the degeneration and regeneration of the cerebral ganglion complex of the ascidian Styela plicata following injection of the niacinamide antagonist 3-acetylpyridine (3AP), described as targeting the CNS of several vertebrates. For the analysis and establishment of a new model in ascidians, the ganglion complex was dissected and prepared for transmission electron microscopy (TEM), routine light microscopy (LM), immunohistochemistry and Western blotting, 1 or 10 days after injection of 3AP. The siphon stimulation test (SST) was used to quantify the functional response. One day after the injection of 3AP, CNS degeneration and recruitment of a non-neural cell type to the site of injury was observed by both TEM and LM. Furthermore, weaker immunohistochemical reactions for astrocytic glial fibrillary acidic protein (GFAP) and neuronal βIII-tubulin were observed. In contrast, the expression of caspase-3, a protein involved in the apoptotic pathway, and the glycoprotein CD34, a marker for hematopoietic stem cells, increased. Ten days after the injection of 3AP, the expression of markers tended toward the original condition. The SST revealed attenuation and subsequent recovery of the reflexes from 1 to 10 days after 3AP. Therefore, we have developed a new method to study ascidian neural degeneration and regeneration, and identified the decreased expression of GFAP and recruitment of blood stem cells to the damaged ganglion as reasons for the success of neuroregeneration in ascidians.

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Section: Resultssupporting

confidence: 67%

3‐acetylpyridine‐induced degeneration in the adult ascidian neural complex: Reactive and regenerative changes in glia and blood cells

Medina

Abreu

Cavalcante

et al. 2014

Developmental Neurobiology

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“…The monoclonal antibody used was against glial fibrillary acidic protein (GFAP,-antirabbit IgG; Sigma), diluted at 1:100, which labels glial cells (da Silva et al 2004). The secondary antibody was Alexa 546 (goat anti-rabbit) diluted at 1:600.…”

Section: Histochemistry and Immunohistochemistrymentioning

confidence: 99%

Identity of the cells recruited to a lesion in the central nervous system of a decapod crustacean

et al. 2010

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In a previous study, we analyzed and described the features of the degeneration of the protocerebral tract (PCT) of the crustacean Ucides cordatus, after the extirpation of the eyestalk. In that study, among axons with axoplasmic degeneration, cells with granules resembling blood cells (hemocytes) were seen. Therefore, in the present study, we characterized the circulating hemocytes and compared them with the cells recruited to a lesion, which was produced as in the former study. Using histochemistry, immunohistochemistry, and electron microscopy (transmission and scanning), we confirmed that circulating and recruited cells display a similar morphology. Therefore, in the crab, hemocytes were attracted to the lesion site in the acute stage of degeneration, appearing near local glial cells that showed signs of being responsive. Some of the attracted hemocytes displayed a morphology that was considered to be possibly activated blood cells. Also, the cells that migrated to the injured PCT displayed features, such as the presence of hydrolytic enzymes and an ability to phagocytize neural debris, similar to those of vertebrates. In summary, our results indicate that hemocytes were not only phagocytizing neural debris together with glial cells but also that they may be concerned with creating a favorable environment for regenerating events.

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“…The nitrocellulose membrane containing the immobilized proteins was first blocked with non-fat dry milk (5%) plus BSA (1%) in 0.1 M TRIS-buffered saline containing 0.001% Tween (TBS t ), for 90 min. This procedure had previously been used successfully in blotting proteins obtained from the eyestalks of crabs of the same species da Silva et al 2004). After blockage, the membrane was washed twice in TBS t under constant stirring for 3 min.…”

Section: Protein Determination Assaymentioning

confidence: 99%

Catalase, Bax and p53 expression in the visual system of the crab Ucides cordatus following exposure to ultraviolet radiation

et al. 2007

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In invertebrates, a few studies have suggested apoptosis as the mechanism of choice to protect the retina after exposure to ultraviolet (UV) radiation. We demonstrated previously, by electron microscopy, that the retina and lamina ganglionaris (or lamina) cells of the crab Ucides cordatus displayed subcellular signs of apoptosis after exposure to UVB and UVC. Here, we first ascertained, by the TdT-mediated dUTP-biotin nick end-labeling (TUNEL) technique, that UV irradiation indeed produced the previously reported results. We next tested, in the visual system of U. cordatus, whether the expression (as analyzed by immunohistochemistry and observed with laser scanning microscopy) and levels (as examined by Western blotting) of catalase, Bax, and p53 were affected by the same dose of UV radiation as that used previously. Our data revealed that the intensity of catalase, Bax, and p53 labeling was stronger in irradiated retina and lamina cells than in non-irradiated retina and lamina. However, no significant difference was observed in the concentrations of these proteins isolated from the whole optic lobe. The results thus suggest that UVB and UVC induce apoptosis in the crustacean retina and lamina by increasing catalase expression and activating the Bax- and p53-mediated apoptosis pathways.

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Glial fibrillary acidic protein (GFAP)‐like immunoreactivity in the visual system of the crab Ucides cordatus (Crustacea, Decapoda)

Cited by 13 publications

References 41 publications

3‐acetylpyridine‐induced degeneration in the adult ascidian neural complex: Reactive and regenerative changes in glia and blood cells

3‐acetylpyridine‐induced degeneration in the adult ascidian neural complex: Reactive and regenerative changes in glia and blood cells

Identity of the cells recruited to a lesion in the central nervous system of a decapod crustacean

Catalase, Bax and p53 expression in the visual system of the crab Ucides cordatus following exposure to ultraviolet radiation

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