Morphological investigation of the gills of the dusky grouper <i>Epinephelus marginatus</i> (Lowe 1834) using gross anatomy and scanning electron microscopy

Alsafy, Mohamed; El‐Gendy, Samir

doi:10.1002/jemt.24050

Cited by 16 publications

(13 citation statements)

References 23 publications

(83 reference statements)

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“…The current research has determined that C. carpio possesses four‐gill arches on either side of its body. These gill arches are arranged in a lateral to medial sequence, similar to the majority of teleost fishes; S. luridus , P. pagrus , B. boops (Alsafy, Seif, et al, 2022), Epinephelus marginatus (Alsafy & El‐Gendy, 2022), Sphyraena sphyraena (Hanafy, 2022), Chaca chaca (Mistri et al, 2016), Corydoras paleatus (Plaul et al, 2021), Siniperca chuatsi (Tran et al, 2022), Labeotropheus fuelleborni , Tropheops (Zogbaum et al, 2021). They are designated as the first, second, third and fourth gills.…”

Section: Discussionmentioning

confidence: 98%

“…The morphological alterations that fish undergo in response to their diet and surroundings indicate their capacity to swiftly acclimate to the evolving environment (Alsafy & El‐Gendy, 2022; Demirci et al, 2021). The morphology of gill arches and gill rakers is useful for understanding fish species differentiation and identifying new species (Kovalchuk & Barkaszi, 2021).…”

Section: Discussionmentioning

confidence: 99%

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Gill arch and raker morphology of common carp (Cyprinus carpio, Linnaeus, 1758) sampled in aquaculture system

Demirci,

Kesbiç

2023

Anat Histol Embryol

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The study aimed to investigate the morphologic aspects of common carp's gill arch and gill rakers (Cyprinus carpio, Linnaeus, 1758), an omnivore and highest‐produced aquaculture species. The study used 10 common carp (395.35 ± 45.06 g) grown entirely under aquaculture conditions. The fish tissues were fixed with Glutaraldehyde (2.5%) for scanning electron microscopy and with formalin (10%) for stereomicroscopic examination. In the SEM examination, two types of taste papillae (Type II and Type III) were observed in the pharyngeal mucosa. Microridge‐like structures in the epithelial layer were found to have two forms. The study findings indicate a significant decrease in gill arch lengths from cranial to caudal and a significant increase in rakers per unit area, as determined through digital calliper measurements and stereomicroscopic examinations (p < 0.05). However, there was no significant difference in measurements of gill arches and raker numbers between the bilateral symmetry of the gill arches (p > 0.05). In conclusion, it was observed that the epithelial structure on the common carp gill arch contained two types of microridge‐like structures: the gill arch length decreased from cranial to caudal, and the rake density on these arches increased caudally.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Gill arch and raker morphology of common carp (Cyprinus carpio, Linnaeus, 1758) sampled in aquaculture system

Demirci,

Kesbiç

2023

Anat Histol Embryol

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“…The obtained SEM images and light photomicrographs were investigated by Image J (Wayne Rasband and contributors, National Institutes of Health, USA) to measure the different lengths of different setae types, and the mean ± standard error were calculated using Microsoft Excel (Alsafy & El‐Gendy, 2022; El‐Bakary et al, 2023).…”

Section: Methodsmentioning

confidence: 99%

Light and scanning electron microscopic characterization of the Egyptian buffalo hair in relation to age with analysis by SEM‐EDX

El‐Gendy

Derbalah

El‐Mansi

et al. 2023

Microscopy Res & Technique

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The purpose of this study was to demonstrate a relationship between the microstructure and measurements of Egyptian buffalo hair and age. The buffalo studied ranged in age from young to premature to adult (3–8 months, 1.5–3, 4–6, and 8–10 years). The hair was collected from the animals' withers. Cuticle elements were examined using scanning electron microscopy (SEM), light microscopy, and energy‐dispersive X‐ray (EDX) analysis. Hair shaft diameter increased with age, according to SEM and light microscopy measurements. The values of shaft diameter obtained by SEM of the same animal were 64%–67% of the values obtained by light microscopy due to shrinkage of the hair during the drying process. Additional microscopy measurements revealed that the width of the cortex and medulla increased with age, while the width of the cuticle decreased slightly. The medulla index of four different age groups ranged from 0.56 to 0.61 μm. The average distance between successive scale values increased from 4.83 μm in the young group to 8.86 μm between the ages of 8 and 10 years. The scale pattern had a distinct personality at each stage of age. The hair medulla was in the center and consisted of a mesh‐like structure with large pores divided into smaller pores by septa. The cortex was a bundle of fibers that wrapped around the medulla. Light microscopy revealed small oval granules and large streak‐like granules in the cortex. EDX spectra revealed that carbon, oxygen, and nitrogen were shown to be more abundant in all age groups of buffalo. In buffalo hair, oxygen was the second most abundant element after carbon. The carbon mass in the examined samples decreased slightly with age (42.31%, 39.18%, 38.88%, and 38.49%), while oxygen increased with age. We concluded that hair measurements varied with age, scale microstructure, and elements, so we estimated buffalo animals' ages up to 10 years. Highlights The goal was to show a link between the microstructure and measurements of Egyptian buffalo hair and age. Hair shaft diameter increased with age, according to scanning electron microscopy (SEM) and light microscopy measurements. The hair shaft diameter obtained by light microscopy was higher than that obtained by scanning electron microscopy of the same animal; we hypothesized that the difference in hair shaft diameter measured by light microscopy and scanning electron microscopy of the same animal was due to the shrinkage of the hair during the drying process. The width of the cortex and medulla increased with age, while the width of the cuticle decreased slightly. The average distance between successive scale values increased from 4.83 μm in the young group to 8.86 μm between 8 and 10 years old. At each stage of age, the scale pattern had a distinct personality. Carbon, oxygen, and nitrogen were found to be more abundant in the hair of buffalo of all ages and were detected using energy‐dispersive X‐ray (EDX) spectra. After carbon, oxygen was the second most abundant element in buffalo hair. The carbon mass in the samples studi...

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“…The CT images were analyzed using the ImageJ 1.53 k application (National Institutes of Health, USA) to measure the Ossa cordis using the previously described method (Alsafy & El‐Gendy, 2022 ; Moittie et al, 2020 ; Witkowska et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Ossa cordis and os aorta in the one‐humped camel: Computed tomography, light microscopy and morphometric analysis

El‐Gendy

Alsafy

Elarab

et al. 2022

Microscopy Res & Technique

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The present study describes the morphological characteristics of the camel heart Ossa cordis, and os aorta using computed tomography soft tissue window (CT) alongside 3D render volume reconstructions and light microscopy. The current study techniques demonstrated the Ossa cordis and os aorta in the cardiac window with more precision than the black and white (ghost), and angiography images. Transverse and sagittal CT images additionally demonstrated the presence of Ossa cordis and os aorta. This study is the first to record two small Ossa cordis sinistrum and one os aorta in the camel heart, in addition to the more commonly observed singular, large, os cordis dextrum. The os cordis dextrum was always located in the upper part of the interventricular septum, near to its junction with the atrium, forming an elongated rectangular shape when observed transversally. The wider cranial part was composed from bone, whereas the caudal aspect was narrow and contained both bone and cartilage. Light microscopy identified that the os cordis dextrum consisted of trabecular bone, marrow spaces, and hyaline cartilage.Two Ossa cordis sinistrum were detected on the left side of the heart, one in the right fibrous ring and another in the interventricular septum, microscopy showed that both contained only trabecular bone with osteocytes, osteoblasts, and osteoclasts. At the level of ascending aorta, there was also trabecular bone containing osteocytes, an os aorta.

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Morphological investigation of the gills of the dusky grouper Epinephelus marginatus (Lowe 1834) using gross anatomy and scanning electron microscopy

Cited by 16 publications

References 23 publications

Gill arch and raker morphology of common carp (Cyprinus carpio, Linnaeus, 1758) sampled in aquaculture system

Gill arch and raker morphology of common carp (Cyprinus carpio, Linnaeus, 1758) sampled in aquaculture system

Light and scanning electron microscopic characterization of the Egyptian buffalo hair in relation to age with analysis by SEM‐EDX

Ossa cordis and os aorta in the one‐humped camel: Computed tomography, light microscopy and morphometric analysis

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