Morphological Identification Nematodes Tanqua tiara Found on Gastric Varanus salvator at East Java

Agustin, Alfiana Laili Dwi; Koesdarto, Setiawan; Lukiswanto, Bambang Sektiari; Suwanti‬, ‪Lucia Tri; Arifin, Zainal; Putranto, Emmanuel Djoko

doi:10.18502/kls.v3i6.1196

Cited by 4 publications

(7 citation statements)

References 11 publications

(14 reference statements)

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“…Although distribution of V. salvator in Bangladesh is in mangroves this species also frequent, ponds, swamps, sewers and drains which reflects the habitat where C. punctata is commonly distributed ( Froese and Pauly, 2018 ). Eggs shed in the faeces of V. salvator infect multiple aquatic species ( Agustin et al, 2017 ) many of which are also foods predated by C. punctata ( Deshmukh et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

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Genetic characterisation of Tanqua (von Linstow, 1879) (Nematoda: Gnathostomatidae) larval forms including new host and locality records

Williams

Hernández‐Jover

Hossen

et al. 2022

International Journal for Parasitology: Parasites and Wildlife

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In an unrelated study of spotted snakehead fish Channa punctata (Bloch) of family Channidae (N = 103) from Bangladesh, ten fish had taupe and clear coloured cysts attached to the intestinal mesentery. Investigation of the cysts revealed larval nematodes. The larvae were damaged and not suitable for detailed morphological study, however, key features such as tooth like projections of the pseudolabia and lateral pseudolabium were observed in specimens with undamaged cephalic regions. Molecular characterisation was undertaken and although the parasite genetic material was poor, five of the twelve nematode larvae through sequencing of the 18S ribosomal RNA gene, showed 98.17% match with sequences assigned for Tanqua tiara (accession number JF934728) deposited in GenBank. The prevalence of infection was 9.7% and the mean intensity 2.70. Tanqua has not previously been identified in fish, or from the definitive host, the Asian water monitor Varanus salvator (Laurenti, 1768) of family Varanidae (class Reptilia), in Bangladesh. Therefore, this study represents a new host and locality record for this nematode species. In many previous reports from this region, nematode larvae have been identified morphologically and assigned to a diverse range of nematode genera. Some confusion therefore exists regarding their accuracy and further investigations are required using molecular methodology to clarify the species of larval nematodes which infect edible fish in Bangladesh.

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Section: Discussionmentioning

confidence: 99%

“… Agustin et al (2017) speculated that T. tiara has zoonotic potential and mammals may become infected from drinking worm eggs in contaminated water ( Agustin et al, 2017 ). The authors of the present publication found no evidence to support this, however T. tiara is a species of one of four genera within the family Gnathostomatidae.…”

Section: Discussionmentioning

confidence: 99%

Genetic characterisation of Tanqua (von Linstow, 1879) (Nematoda: Gnathostomatidae) larval forms including new host and locality records

Williams

Hernández‐Jover

Hossen

et al. 2022

International Journal for Parasitology: Parasites and Wildlife

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“…Tanqua tiara is a long-shaped nematode and can be distinguished between the male and female. Investigation by Agustin et al (2017), T. tiara which infected Varanus salvator from East Java showed the male animal has total length of 9.4-32.0 mm with body width of 0.26-1.77 mm, while the female animal has total length of 6.8-22.0 mm with body width of 0.14-2.33 mm. Male T. tiara which infected V. salvator and V. magna was reported to have total length of 13.8 -28.6 mm with body width of 0.39-0.77 mm, while the female has total length of 13.0-25.7 mm with body width of 0.26-0.42 mm (Purwaningsih et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Record on Nematode <I>Tanqua tiara</I> Infection on Snakehead Fish <I>Channa striata</I> in South Kalimantan Indonesia

Murwantoko

Hayati²

2022

JIPK

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Highlight Research The parasitic disease has been record in snakehead fish (Channa striata) from South Kalimantan The nematodes with needle shape on both tip end with approximately 1 mm length and moving inside of wall cysts were found in in abdomen cavity and flesh of fish The morphology of the nematode was observed by light- and scanning electron-microscope The analysis on 18S rRNA showed that this parasite is belonging to nematode Tanqua tiara Abstract Snakehead fish (Channa striata) is an important commodity in South Kalimantan Indonesia. The snakehead fish production was increased due to the capture and intensive culture. The disease is one of the obstacles for production that may happened in cultured- and wild-fishes. The aims of this study were to record and to identify parasite which infected on wild snakehead fish from Kandangan Lama, Panyipatan, Tanahlaut, South Kalimantan. The parasite identification was conducted based on the morphology and the molecular characters. The morphology was observed by light microscope and scanning electron microscope. The 18S rRNA of parasite was amplified using designed primers and followed by sequencing. Spherical cysts were found in abdomen cavity and flesh of snakehead fish. The cylindrical worm with needle shape on both tip end with approximately 1 mm length were moving inside of wall cysts. Alignment analysis of 18S rRNA showed the highest homology at 99.83% with Tanqua tiara. Phylogenic tree showed that this worm is located at distance clade with the nematodes that have been reported to infect snakehead fish. The morphology and molecular results verified that and first report the parasite found in snakehead fish in South Kalimantan was T. tiara species. This nematode parasite may be served as intermediate host.

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“…Hung’s II solution was dripped sufficiently over the worm and covered with a cover glass. Slides were dried in the incubator at 37°C [ 15 ]. Worms were observed using a light microscope based on the method of Soota and Chaturvedi [ 7 ] with 40 and 100×.…”

Section: Methodsmentioning

confidence: 99%

Morphologic and morphometric characteristics of ascaroid worm, Ophidascaris piscatori in Xenochrophis piscator snake in Sidoarjo, Indonesia

Suwanti‬¹,

Audini²,

Koesdarto³

et al. 2018

Vet World

Self Cite

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Aim:The study was conducted to describe the morphology and morphometry of nematode worm in the stomach of Xenochrophis piscator snake macroscopically and microscopically using light and scanning electron microscopy (SEM).Materials and Methods:The sample was 40 nematode worms that have been collected from 60 snakes which slaughtered at snake slaughterhouses in Sidoarjo, Indonesia. The worms (10 male and 10 female) were stained with carmine, and the others were sent to Indonesian Science Institute for ultrastructure observation by SEM. Some of the female worms were immersed in physiological NaCl and incubated to collect the worm eggs.Results:Nematode worm in this study had three lips with almost the same size and it had papillae, so it was included in ascaridoid. The mouth of ascaridoids has three lips, the dorsal bearing two large outer papillae and the each lateroventral with one papilla. The body length and width of the male worm were 70-105 mm and 0.92-1.32, respectively, with head diameter of 0.22-0.28 mm. Dorsal and ventrolateral lips almost have the same size that was 0.10-0.12×0.11-0.13 mm. The length of interlabia was 0.06-0.08 mm, esophagus was 3.21-4 mm, tail was 0.17-0.23 mm, and spicule was 2.12-3.36 mm. The body length and width of the female worm were 85-130 mm and 1.28-1.71 mm, respectively. The head diameter was 0.29-0.38 mm. Dorsal and ventrolateral lips almost have the same size that was 0.13-0.16×0.15-0.19 mm. The interlabial length was 0.08-0.10 mm, esophagus was 3.04-4.67 mm, and tail was 0.22-0.31 mm. The distance of the vulva from the anterior edge was 56-88 mm with an average of 67.35 mm. The eggs have conspicuously pitted with length 0.08-0.09 mm and width 0.07-0.08 mm.Conclusion:Based on the characteristics of morphology and morphometry, the ascaroid worms found on X. piscator snake from Sidoarjo, Indonesia, were O. piscatori.

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Morphological Identification Nematodes Tanqua tiara Found on Gastric Varanus salvator at East Java

Cited by 4 publications

References 11 publications

Genetic characterisation of Tanqua (von Linstow, 1879) (Nematoda: Gnathostomatidae) larval forms including new host and locality records

Genetic characterisation of Tanqua (von Linstow, 1879) (Nematoda: Gnathostomatidae) larval forms including new host and locality records

Record on Nematode <I>Tanqua tiara</I> Infection on Snakehead Fish <I>Channa striata</I> in South Kalimantan Indonesia

Morphologic and morphometric characteristics of ascaroid worm, Ophidascaris piscatori in Xenochrophis piscator snake in Sidoarjo, Indonesia

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