Morphological features of bone healing under the effect of collagen-graft—glycosaminoglycan copolymer supplemented with the tripeptide Gly-His-Lys

Pohunková, H; Stehlík, J; Vachal, J; Cech, O; Adam, M.

doi:10.1016/0142-9612(95)00310-x

Cited by 22 publications

(13 citation statements)

References 11 publications

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“…With the exception of hyaluronan, all of these (heparan sulfate, chondroitin sulfate, keratan sulfate, and dermatan sulfate) are sulfated branch‐chained molecules that are conjugated to proteins in the form of proteoglycans. Hyaluronan, an almost ubiquitous component of ECM, has been reported to display both osteoinductive and osteoconductive properties in live animal models (115,116) . With in vitro cultured MSCs, hyaluronan has also been shown to be a potent inducer of osteogenic differentiation (117,118) .…”

Section: Naturally Occurring and Artificially Synthesized Extracellulmentioning

confidence: 99%

“…Hyaluronan, an almost ubiquitous component of ECM, has 1383 OSTEOGENIC DIFFERENTIATION OF STEM CELLS been reported to display both osteoinductive and osteoconductive properties in live animal models. (115,116) With in vitro cultured MSCs, hyaluronan has also been shown to be a potent inducer of osteogenic differentiation. (117,118) For tissue engineering applications, hyaluronan-based matrix scaffolds have been fabricated, and these have been shown to be efficient carrier vehicles for osteogenic differentiation and mineralization of bone marrow-derived MSCs.…”

Section: Naturally Occurring and Artificially Synthesized Extracellulmentioning

confidence: 99%

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Strategies for Directing the Differentiation of Stem Cells Into the Osteogenic Lineage In Vitro

Heng

Cao

Stanton

et al. 2004

Journal of Bone and Mineral Research

152

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A major area in regenerative medicine is the application of stem cells in bone reconstruction and bone tissue engineering. This will require well-defined and efficient protocols for directing the differentiation of stem cells into the osteogenic lineage, followed by their selective purification and proliferation in vitro. The development of such protocols would reduce the likelihood of spontaneous differentiation of stem cells into divergent lineages on transplantation, as well as reduce the risk of teratoma formation in the case of embryonic stem cells. Additionally, such protocols could provide useful in vitro models for studying osteogenesis and bone development, and facilitate the genetic manipulation of stem cells for therapeutic applications. The development of pharmokinetic and cytotoxicity/genotoxicity screening tests for bone-related biomaterials and drugs could also use protocols developed for the osteogenic differentiation of stem cells. This review critically examines the various strategies that could be used to direct the differentiation of stem cells into the osteogenic lineage in vitro.

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Section: Naturally Occurring and Artificially Synthesized Extracellulmentioning

confidence: 99%

Section: Naturally Occurring and Artificially Synthesized Extracellulmentioning

confidence: 99%

Strategies for Directing the Differentiation of Stem Cells Into the Osteogenic Lineage In Vitro

Heng

Cao

Stanton

et al. 2004

Journal of Bone and Mineral Research

152

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“…Because of the excellent biocompatibility of collagen, the use of porous collagen scaffolds as a regeneration template for tissues such as skin,1–3 cartilage,4, 5 bones,6 and nerve7, 8 has been widely researched. However, the fast biodegradation rate and low mechanical strength of the untreated collagen cannot meet the demands of in vitro or in vivo applications in many cases, and have been the crucial factors that limit the further use of this material 9, 10…”

Section: Introductionmentioning

confidence: 99%

Biodegradability and cell‐mediated contraction of porous collagen scaffolds: The effect of lysine as a novel crosslinking bridge

Gao

Mao

et al. 2004

J Biomedical Materials Res

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A novel crosslinking method was adopted to modify the porous collagen scaffolds by using a water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDAC) and N-hydroxysuccinimide (NHS) in the presence of lysine, which functions as a crosslinking bridge. In vitro biodegradation tests proved that in the presence of lysine the biostability of the EDAC crosslinked scaffolds was greatly enhanced. The biostability of the resultant scaffolds was also elucidated as a function of the concentrations of lysine and EDAC/NHS. Compared to the Col-DHT, the ability of the Col-EDAC and the Col/Lys to resist cell-mediated contraction (CMC) was greatly enhanced. Yet no obvious difference between the Col-EDAC and the Col/Lys was found with respect to CMC. SEM observations showed that the microstructure of the crosslinked scaffolds could be largely preserved after fibroblast seeding. As a result, MTT assays proved that the fibroblasts in the Col/Lys scaffolds proliferated faster compared to the DHT-treated one on the assumption that the cell viability was preserved to a similar level. Histological section results indicated that the Col/Lys scaffolds had the ability to accelerate the cell infiltration and proliferation. All these results demonstrated that this novel crosslinking method is an effective way to achieve a collagen scaffold with improved biostability and a more stable structure, which can resist cell-mediated contraction. (c) 2004 Wiley Periodicals, Inc. J Biomed Mater Res 71A: 334-342, 2004.

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“…In addition to its use in cosmetics, previous reports describe efforts to deliver GHK locally for stimulating bone repair [36, 37]. However, GHK was physically associated with collagen gels, thereby allowing the rapid diffusion away from the implant site.…”

Section: Discussionmentioning

confidence: 99%

Enhanced trophic factor secretion by mesenchymal stem/stromal cells with Glycine-Histidine-Lysine (GHK)-modified alginate hydrogels

et al. 2014

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Recombinant proteins and cytokines are under broad preclinical and clinical investigation to promote angiogenesis, but their success is limited by ineffective delivery, lack of long-term stability, and excessive cost. Mesenchymal stem/stromal cells (MSC) secrete bioactive trophic factors, and thus, may provide an effective alternative to address these challenges. Glycine-Histidine-Lysine (GHK) is a peptide fragment of osteonectin (SPARC), a matricellular protein with reported proangiogenic potential. We examined the capacity of GHK to upregulate secretion of proangiogenic factors from human MSC in culture and when covalently coupled to alginate hydrogels. GHK had no apparent cytotoxic effects on MSC in culture over a wide range of concentrations. We detected a dose-dependent increase in vascular endothelial growth factor (VEGF) concentration in media conditioned by GHK-treated MSC, which increased endothelial cell proliferation, migration, and tubule formation. We covalently coupled GHK to alginate using carbodiimide chemistry, and human MSC were entrapped in alginate hydrogels to assess VEGF secretion. Similar to monolayer culture, MSC responded to GHK-modified gels by secreting increased concentrations of VEGF and basic fibroblast growth factor (bFGF) compared to unmodified gels. The pre-treatment of MSC with antibodies to α6 and β1 integrins prior to entrapment in GHK-modified gels abrogated VEGF secretion, suggesting that the proangiogenic response of MSC was integrin-mediated. These data demonstrate that the proangiogenic potential of MSC can be significantly increased by the presentation of GHK with a biodegradable carrier, therefore increasing their clinical potential when used for tissue repair.

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Morphological features of bone healing under the effect of collagen-graft—glycosaminoglycan copolymer supplemented with the tripeptide Gly-His-Lys

Cited by 22 publications

References 11 publications

Strategies for Directing the Differentiation of Stem Cells Into the Osteogenic Lineage In Vitro

Strategies for Directing the Differentiation of Stem Cells Into the Osteogenic Lineage In Vitro

Biodegradability and cell‐mediated contraction of porous collagen scaffolds: The effect of lysine as a novel crosslinking bridge

Enhanced trophic factor secretion by mesenchymal stem/stromal cells with Glycine-Histidine-Lysine (GHK)-modified alginate hydrogels

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