Biodegradability and cell‐mediated contraction of porous collagen scaffolds: The effect of lysine as a novel crosslinking bridge

Abstract

A novel crosslinking method was adopted to modify the porous collagen scaffolds by using a water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDAC) and N-hydroxysuccinimide (NHS) in the presence of lysine, which functions as a crosslinking bridge. In vitro biodegradation tests proved that in the presence of lysine the biostability of the EDAC crosslinked scaffolds was greatly enhanced. The biostability of the resultant scaffolds was also elucidated as a function of the concentrations of lysine and EDAC/NHS. Compared to the Col-DHT, the ability of the Col-EDAC and the Col/Lys to resist cell-mediated contraction (CMC) was greatly enhanced. Yet no obvious difference between the Col-EDAC and the Col/Lys was found with respect to CMC. SEM observations showed that the microstructure of the crosslinked scaffolds could be largely preserved after fibroblast seeding. As a result, MTT assays proved that the fibroblasts in the Col/Lys scaffolds proliferated faster compared to the DHT-treated one on the assumption that the cell viability was preserved to a similar level. Histological section results indicated that the Col/Lys scaffolds had the ability to accelerate the cell infiltration and proliferation. All these results demonstrated that this novel crosslinking method is an effective way to achieve a collagen scaffold with improved biostability and a more stable structure, which can resist cell-mediated contraction. (c) 2004 Wiley Periodicals, Inc. J Biomed Mater Res 71A: 334-342, 2004.

Evaluation of the ability of collagen–glycosaminoglycan scaffolds with or without mesenchymal stem cells to heal bone defects in Wistar rats

“…Because of the contraction of cell-seeded scaffolds, changes in the size and microstructure of the implants take place frequently. Cell-mediated contraction can collapse the pores of the scaffold, thereby possibly affecting cell migration and proliferation and the flow of nutrients, and can distort the shape of the construct [34][35]. A change in size will cause difficulty in fitting a specific implant site, or cause separation from the surrounding tissue host [36][37].…”

Entrapment of Embryonic Stem Cells-Derived Cardiomyocytes in Macroporous Biodegradable Microspheres: Preparation and Characterization

“…CultiSpher-S beads are suitable for expansion of mesenchymal stem cells and may be applied to regenerate bone and other tissues [8]. Moreover, porous gelatin and collagen spheres have been proven to be valuable in cell-based therapies for the treatment of acute and persistent wounds [10,[20][21][22][23]. A variety of animal and human cell lines have been successfully cultivated in macroporous gelatine spheres [16][17][18].…”

Artocarpin-enriched extract reverses collagen metabolism in UV-exposed fibroblasts

“…Hydroxyproline that is a major component of the collagen in the skin tissue was also measured, according to previous studies (Ma et al 2004;Faikrua et al 2009). Briefly, 25 mg of the tissue sample was immersed in chloroform and methanol for 60 min.…”