Morphological Expression of
            <i>KIT</i>
            Positive Interstitial Cells of Cajal in Human Bladder

Johnston, Louise; Woolsey, Siobhan; Cunningham, Rebecca M.; O'Kane, Hugh; Duggan, Brian; Keane, Patrick F.; McCloskey, Karen

doi:10.1016/j.juro.2010.03.005

Cited by 64 publications

(68 citation statements)

References 29 publications

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“…We and others recently demonstrated that ICC markers are expressed in BICCs, including c-kit, anoctamin 1, gap junction protein connexin 43, and CD34 (88). These cells are closely associated with intramural nerves and BSM cells (89)(90)(91). The overlying schematic in Figure 9 illustrates the position they occupy in the bladder wall.…”

Section: Bladder Icc: An Old Cell Newly Implicated In Lower Urinary Tmentioning

confidence: 94%

Control of Urinary Drainage and Voiding

Hill

2015

Clinical Journal of the American Society of Nephrology

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Urine differs greatly in ion and solute composition from plasma and contains harmful and noxious substances that must be stored for hours and then eliminated when it is socially convenient to do so. The urinary tract that handles this output is composed of a series of pressurizable muscular compartments separated by sphincteric structures. With neural input, these structures coordinate the delivery, collection, and, ultimately, expulsion of urine. Despite large osmotic and chemical gradients in this waste fluid, the bladder maintains a highly impermeable surface in the face of a physically demanding biomechanical environment, which mandates recurring cycles of surface area expansion and increased wall tension during filling, followed by rapid wall compression during voiding. Afferent neuronal inflow from mucosa and submucosa communicates sensory information about bladder fullness, and voiding is initiated consciously through coordinated central and spinal efferent outflow to the detrusor, trigonal internal sphincter, and external urethral sphincter after periods of relative quiescence. Provocative new findings suggest that in some cases, lower urinary tract symptoms, such as incontinence, urgency, frequency, overactivity, and pain may be viewed as a consequence of urothelial defects (either urothelial barrier breakdown or inappropriate signaling from urothelial cells to underlying sensory afferents and potentially interstitial cells). This review describes the physiologic and anatomic mechanisms by which urine is moved from the kidney to the bladder, stored, and then released. Relevant clinical examples of urinary tract dysfunction are also discussed.

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Section: Bladder Icc: An Old Cell Newly Implicated In Lower Urinary Tmentioning

confidence: 94%

Control of Urinary Drainage and Voiding

Hill

2015

Clinical Journal of the American Society of Nephrology

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“…They suggested that existence and survival of ICC mainly rely on neural release of trophic factors. [12] A recent study by Johnston et al [6] showed that the human bladder contains a network of ICCs in the lamina propria, which is connected to cholinergic nerve plexus. Perivascular ICCs are localized close to the vascular smooth muscle cells, whereas the detrusor ICCs are observed in smooth muscle bundles and associated with nerves, controlling the bladder contractility.…”

Section: Discussionmentioning

confidence: 99%

“…Perivascular ICCs are localized close to the vascular smooth muscle cells, whereas the detrusor ICCs are observed in smooth muscle bundles and associated with nerves, controlling the bladder contractility. [6] Piaseczna-Piotrowska et al [13] had examined the distribution of ICCs in different parts of the overactive neurogenic bladder wall of the children with MMC. They had found that the ICC are widely located in the trigone than the anterior wall of the corpus of the urinary bladder.…”

Section: Discussionmentioning

confidence: 99%

“…[3,4] The interaction between the smooth muscle and neural cells is mediated through the interstitial cells of Cajal (ICC) which also controls bladder contractility. [5,6] Interstitial cells of Cajal are the pacemaker cells found in human organs with peristaltic activity that were thought to be originating from the precursors of the smooth muscle cells. [7,8] Several studies have shown that ICCs are widely distributed in the urinary tract of animals and humans.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of interstitial cells of Cajal in the bladders of fetal rats with retinoic acid induced myelomeningocele

Tekın

Karakuş

Hakgüder

et al. 2016

Turkish Journal of Urology

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Objective: Myelomeningocele (MMC) is one of the most common reason of neurogenic bladder dysfunction in children. Although neurogenic bladder dysfunction occurrence is related with bladder innervation, also there are some changes seen in the smooth muscle and neural cells of the bladder. Interstitial cells of Cajal (ICC) are the pacemaker cells found in organs with peristaltic activity. Although it has been shown that ICC are diminished in the rat urinary bladder with traumatic spinal cord injury, there is no data about ICC in fetal rat bladders with MMC. This study has been conducted to investigate the ICC in the bladders of fetal rats with retinoic acid induced MMC. Materials and methods:Time dated pregnant Wistar albino rats were divided into 3 groups. In MMC group, dams were fed with gavage solution containing 60 mg/kg all-trans retinoic acid dissolved in olive oil on 10. embryologic day. Sham group animals were fed only olive oil. Control group dams were fed with standard rat chow. Fetuses were delivered by cesarean section and harvested on 22. embryologic day. MMC was identified by observing MMC sacs at the back of the fetuses. Distribution of ICCs were evaluated using immunohistochemical staining.Results: ICCs were found in all groups, which have the same morphological features that had been described earlier in the gastrointestinal tract and the bladder. The density of the ICC in the MMC group was found to be significantly decreased when compared with the control and the sham groups (p<0.05). Conclusion:The density of the ICC in the urinary bladder decreased in the neurogenic bladder developed in MMC.

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“…It is known that c-kit is a specific marker for ICs [4,8,12]. Additionally, antibodies targeting connexin 43 [8,14] and vimentin [8,15] molecules were also used to stain ICs in the human urinary tract. Therefore, we used antibodies targeting c-kit, connexin 43 and vimentin in order to stain and detect ICs in the human urinary tract tissues.…”

Section: Introductionmentioning

confidence: 99%

498 Does Diabetes Affect Distribution and Number of Interstitial Cells and Neuronal Tissue in the Ureter, Bladder, Prostate and Urethra of Humans?

Canda¹,

Doğan²,

Kandemir³

et al. 2012

Journal of Urology

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IntroductionThe aim of this study was to investigate and compare the distribution and number of interstitial cells (ICs) and neuronal tissue in the ureter, bladder, prostate, and urethra of human patients with and without diabetes. Material and methods Human tissue was obtained from patients who had undergone radical cystectomy for bladder cancer (10 diabetic and 11 non-diabetic males). Interstitial cells were stained immunohistochemically with anti-human CD117 (c-kit) rabbit polyclonal antibody, Vimentin, and Connexin-43. Neural tissue was stained with synaptophysin. The number of ICs and neurons was evaluated and compared between the groups (diabetic versus non-diabetic). Results The mean number of c-kit (+) ICs in bladder lamina propria was significantly decreased in diabetics (32.40 ±12.96 versus 57.18 ±25.37, p = 0.036). The mean number of ICs in the detrusor muscle was significantly decreased in diabetics (40.50 ±16.79 versus 64.55 ±22.08, p = 0.013). Between the groups, no significant differences were detected regarding the number of ICs at the level of the ureter, urethra, and prostate. No significant differences were detected regarding the number of nerves in the ureter, bladder, prostate, and urethra of both groups. Conclusions The number of ICs may be decreased in the lamina propria and detrusor muscle of the human bladder in diabetes. This can be an underlying cause of lower urinary tract (LUT) dysfunction in diabetics. Research into the development of drugs targeting or stimulating IC function in order to prevent diabetic LUT dysfunction is warranted.

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Morphological Expression of KIT Positive Interstitial Cells of Cajal in Human Bladder

Cited by 64 publications

References 29 publications

Control of Urinary Drainage and Voiding

Control of Urinary Drainage and Voiding

Distribution of interstitial cells of Cajal in the bladders of fetal rats with retinoic acid induced myelomeningocele

498 Does Diabetes Affect Distribution and Number of Interstitial Cells and Neuronal Tissue in the Ureter, Bladder, Prostate and Urethra of Humans?

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