Distribution of interstitial cells of Cajal in the bladders of fetal rats with retinoic acid induced myelomeningocele

Tekın, Ali; Karakuş, Osman Zeki; Hakgüder, Gülce; Ateş, Oğuz; Özer, Erdener; Olguner, Mustafa; Akgür, Feza M.

doi:10.5152/tud.2016.98474

Cited by 6 publications

(9 citation statements)

References 27 publications

(39 reference statements)

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“…Impairment of bladder wall muscular innervation can be seen as early as embryonic day 20 in fetal rats with MMC and persists at term [33]. Additionally, decreased concentrations of interstitial cells of Cajal, which mediate bladder contractility, have been noted in retinoic acid-induced MMC in rats [35].…”

Section: Discussionmentioning

confidence: 99%

Early investigations into improving bowel and bladder function in fetal ovine myelomeningocele repair

Theodorou

Jackson

Stokes

et al. 2022

Journal of Pediatric Surgery

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Section: Discussionmentioning

confidence: 99%

Early investigations into improving bowel and bladder function in fetal ovine myelomeningocele repair

Theodorou

Jackson

Stokes

et al. 2022

Journal of Pediatric Surgery

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“…Under these conditions, the ICCs of the dome wall act as pacemaker cells leading to the detrusor instability. However, Tekin A, et al [ 46 ] found that the density of ICCs decreased in the neurogenic bladder of fetal rats with myelomeningocele. This phenomenon was explained as follows.…”

Section: Discussionmentioning

confidence: 99%

The Dome Wall of Bladder Acts as a Pacemaker Site in Detrusor Instability in Rats

et al. 2017

Med Sci Monit

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BackgroundThe aim of this study was to confirm that the interstitial cells of Cajal (ICCs) in the dome wall of the bladder are pacemaker cells, and that the dome wall of the bladder acts as a pacemaker site in the detrusor instability (DI) rat model.Material/MethodsThe model of DI in Wistar rats was established and urodynamic studies measuring the bladder volume and pressure were performed. The detrusor excitability was investigated using the amplitude and frequency of phasic contraction of strips. The localization and quantity of ICCs was identified by immunohistochemistry and c-KIT protein expression in the rat bladder. PCR assay and Western blot were used to assess the expression of HCN2 and Cx43.ResultsThe bladder capacity, residual volume, voiding volume, and maximum voiding pressure were significantly increased in the DI group. The contraction frequency and amplitude of the strips from the dome of the bladder in the DI group were higher than the triangle, body, and base parts. Both the concentration of c-KIT positive ICCs cells and expression of the c-KIT protein in the dome wall were higher than in other parts of the bladder. The expression of HCN2 and Cx43 in each part of the DI rat group were obviously higher than each part in the control group. Compared to the body, base, and triangle parts, the expression of HCN2 and Cx43 in the dome wall were obviously higher in the DI group.ConclusionsThe quantity of ICCs was higher in the dome wall and the dome wall of bladder acts as a pacemaker site in the DI rat model.

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“…Furthermore, increased gap junction formation in the suburothelium was demonstrated, and it is hypothesized that this change could play a significant role in the sensory and motor pathway of the detrusor abnormality, as the mucosa may take over the neuronal role [ 46 ]. Fetal rats with induced MMC were found to have a significantly reduced number of ICCs, and it was hypothesized, that the observed bladder dysfunction may be related to the reduced density of ICCs in the developing bladder [ 47 ]. The only study performed on children with MMC and NLUTD could not confirm changes in ICC density compared to controls [ 48 ].…”

Section: Structural and Morphological Propertiesmentioning

confidence: 99%

Neurogenic Lower Urinary Tract Dysfunction in Spinal Dysraphism: Morphological and Molecular Evidence in Children

Planta

Gerwinn

Salemi

et al. 2023

IJMS

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Spinal dysraphism, most commonly myelomeningocele, is the typical cause of a neurogenic lower urinary tract dysfunction (NLUTD) in childhood. The structural changes in the bladder wall in spinal dysraphism already occur in the fetal period and affect all bladder wall compartments. The progressive decrease in smooth muscle and the gradual increase in fibrosis in the detrusor, the impairment of the barrier function of the urothelium, and the global decrease in nerve density, lead to severe functional impairment characterized by reduced compliance and increased elastic modulus. Children present a particular challenge, as their diseases and capabilities evolve with age. An increased understanding of the signaling pathways involved in lower urinary tract development and function could also fill an important knowledge gap at the interface between basic science and clinical implications, leading to new opportunities for prenatal screening, diagnosis, and therapy. In this review, we aim to summarize the evidence on structural, functional, and molecular changes in the NLUTD bladder in children with spinal dysraphism and discuss possible strategies for improved management and for the development of new therapeutic approaches for affected children.

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Distribution of interstitial cells of Cajal in the bladders of fetal rats with retinoic acid induced myelomeningocele

Cited by 6 publications

References 27 publications

Early investigations into improving bowel and bladder function in fetal ovine myelomeningocele repair

Early investigations into improving bowel and bladder function in fetal ovine myelomeningocele repair

The Dome Wall of Bladder Acts as a Pacemaker Site in Detrusor Instability in Rats

Neurogenic Lower Urinary Tract Dysfunction in Spinal Dysraphism: Morphological and Molecular Evidence in Children

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