Morphological evidence for a permeability barrier in the testis and spermatic duct of<i>Gymnotus carapo</i>(Teleostei: Gymnotidae)

Souza, Lara C. Meneguelli De; Retamal, Cláudio; Rocha, Gustavo Miranda; Lopez, Maria Luisa

doi:10.1002/mrd.22503

Cited by 8 publications

(8 citation statements)

References 58 publications

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“…For evaluation of the effect of studied mycotoxins on the permeability of the epithelial barrier, we applied the lanthanum technique, which is commonly used in examining tight junctions at the ultrastructural level [ 52 , 53 ]. In the investigated material, electron dense lanthanum particles were observed on the surface of intestine epithelium between the microvilli and in intercellular spaces higher up in the tight junctions.…”

Section: Resultsmentioning

confidence: 99%

The Effects of Deoxynivalenol and Zearalenone on the Pig Large Intestine. A Light and Electron Microscopy Study

Przybylska‐Gornowicz

Lewczuk

Prusik

et al. 2018

Toxins

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The contamination of feed with mycotoxins results in reduced growth, feed refusal, immunosuppression, and health problems. Deoxynivalenol (DON) and zearalenone (ZEN) are among the most important mycotoxins. The aim of the study was to examine the effects of low doses of these mycotoxins on the histological structure and ultrastructure of the large intestine in the pig. The study was performed on 36 immature gilts of mixed breed (White Polish Big × Polish White Earhanging), which were divided into four groups administrated per os with ZEN at 40 µg/kg BW, DON at 12 µg/kg BW, a mixture of ZEN (40 µg/kg BW) and DON (12 µg/kg BW) or a placebo. The pigs were killed by intravenous overdose of pentobarbital after one, three, and six weeks of treatment. The cecum, ascending and descending colon samples were prepared for light and electron microscopy. Administration of toxins did not influence the architecture of the mucosa and submucosa in the large intestine. ZEN and ZEN + DON significantly decreased the number of goblet cells in the cecum and descending colon. The mycotoxins changed the number of lymphocytes and plasma cells in the large intestine, which usually increased in number. However, this effect differed between the intestine segments and toxins. Mycotoxins induced some changes in the ultrastructure of the mucosal epithelium. They did not affect the expression of proliferative cell nuclear antigen and the intestinal barrier permeability. The obtained results indicate that mycotoxins especially ZEN may influence the defense mechanisms of the large intestine.

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Section: Resultsmentioning

confidence: 99%

The Effects of Deoxynivalenol and Zearalenone on the Pig Large Intestine. A Light and Electron Microscopy Study

Przybylska‐Gornowicz

Lewczuk

Prusik

et al. 2018

Toxins

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“…In general, at the spermatogonia stage, the initiation of spermatogenesis, the Sertoli cells begin to extend and dissociate the germ cell, giving rise to a unique microenvironment for synchronous germ cell development within each spermatocyst. The distinctive Sertoli barriers composed by tight junctions and desmosomes could control the movement of materials between the spermatocysts (Fishelson et al, 2006;Meneguelli De Souza & Retamal, 2015). Obvious cytoplasmic bridges were observed among synchronous germ cells within the cysts in this study.…”

Section: Discussionmentioning

confidence: 48%

“…Obvious cytoplasmic bridges were observed among synchronous germ cells within the cysts in this study. Cytoplasmic bridges have also been reported in other fish (Leal et al, 2009;Meneguelli De Souza & Retamal, 2015), suggesting the interrelated and differentiated relationship among those synchronous germ cells within the cysts.…”

Section: Discussionmentioning

confidence: 61%

“…Although spermatogenesis is conserved, variations in the testicular structure and spermatogenesis pattern exist among vertebrates (Sousa et al, 2014). Species of teleost may exhibit variable morphofunctional characteristics of spermatogenesis, including those related to reproductive strategy and behaviour (Fishelson et al, 2006;Meneguelli De Souza & Retamal, 2015). Therefore, this study aimed to describe the ultrastructure of different spermatogenic cells and cellular junctions contributing to spermatogenesis in detail, and morphologically evaluate the testicular development in P. argenteus.…”

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confidence: 99%

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The ultrastructure of spermatogenic cells and morphological evaluation of testicular development in the silver pomfret (Pampus argenteus)

Yang

Wang

et al. 2021

Anat Histol Embryol

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The silver pomfret (Pampus argenteus) is a widely distributed and economically important marine fish in the Indo‐Pacific. In this study, we acquired the second generation of wild P. argenteus by artificial breeding and further studied the testicular development and ultrastructure of spermatogenesis. The results of gonadosomatic index (GSI) showed the spawning period of this marine fish was from April to June. Besides, through morphological analysis, we found that P. argenteus had an anastomosing tubular testis surrounded by a layer of tunica albuginea, in which spermatogenesis occurred in cysts where the synchronous germ cells were completely surrounded by the cytoplasmic projection of Sertoli cells. Meanwhile, based on submicroscopic characteristics, the germ cells are classified into nine different types. During the ontogenesis of testis, both the early stage of spermatogenesis and sperm were observed in P. argenteus. At sperm maturation stage, different types of spermatozoa and activation of sperms occurred non‐synchronously in the tubules. Cytoplasmic bridges also were observed among synchronous germ cells within the cysts, suggesting an interrelated and differentiated relationship among these germ cells.

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“…Histologically, the testicular main duct epithelium is formed by an unfolded monolayer of columnar cells that can show secretory activity, whereas the spermatic duct epithelium can vary between species from a monolayered unfolded epithelium to a multilayered and folded epithelium, the latter increasing the internal surface of the spermatic duct (Lahnsteiner et al 1993a(Lahnsteiner et al ,b, 1994Lahnsteiner, 2003). As in mammals, the epithelial cells of the spermatic duct are generally ciliated (Meneguelli De Souza et al 2015;Melo et al 2016), and surrounded by a stroma containing smooth muscle cells that through their contraction facilitate sperm transport and expulsion (Walter et al 2005).…”

Section: Introductionmentioning

confidence: 99%

The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost

et al. 2018

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Aquaporin-mediated fluid transport in the mammalian efferent duct and epididymis is believed to play a role in sperm maturation and concentration. In fish, such as the marine teleost gilthead seabream (Sparus aurata), the control of fluid homeostasis in the spermatic duct seems also to be crucial for male fertility, but no information exists on the expression and distribution of aquaporins. In this study, reverse transcriptase-polymerase chain reaction and immunoblotting analyses, employing available and newly raised paralog-specific antibodies for seabream aquaporins, indicate that up to nine functional aquaporins, Aqp0a, -1aa, -1ab, -3a, -4a, -7, -8bb, -9b and -10b, are expressed in the spermatic duct. Immunolocalization of the channels in the resting spermatic duct reveals that Aqp0a, -1aa, -4a, -7 and -10b are expressed in the monolayered luminal epithelium, Aqp8b and -9b in smooth muscle fibers, and Aqp1ab and -3a in different interstitial lamina cells. In the epithelial cells, Aqp0a and -1aa are localized in the short apical microvilli, and Aqp4a and -10b show apical and basolateral staining, whereas Aqp7 is solely detected in vesicular compartments. Upon spermiation, an elongation of the epithelial cells sterocilia, as well as the folding of the epithelium, is observed. At this stage, single- and double-immunostaining, using two aquaporin paralogs or the Na /K -ATPase membrane marker, indicate that Aqp1ab, -3a, -7, -8bb and -9b staining remains unchanged, whereas in epithelial cells Aqp1aa translation is supressed, Aqp4a internalizes, and Aqp0a and -10b accumulate in the apical, lateral and basal plasma membrane. These findings uncover a cell type- and region-specific distribution of multiple aquaporins in the piscine spermatic duct, which shares conserved features of the mammalian system. The data therefore suggest that aquaporins may play different roles in the regulation of fluid homeostasis and sperm maturation in the male reproductive tract of fish.

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Morphological evidence for a permeability barrier in the testis and spermatic duct ofGymnotus carapo(Teleostei: Gymnotidae)

Cited by 8 publications

References 58 publications

The Effects of Deoxynivalenol and Zearalenone on the Pig Large Intestine. A Light and Electron Microscopy Study

The Effects of Deoxynivalenol and Zearalenone on the Pig Large Intestine. A Light and Electron Microscopy Study

The ultrastructure of spermatogenic cells and morphological evaluation of testicular development in the silver pomfret (Pampus argenteus)

The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost

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