The ultrastructure of spermatogenic cells and morphological evaluation of testicular development in the silver pomfret (Pampus argenteus)

Abstract

The silver pomfret (Pampus argenteus) is a widely distributed and economically important marine fish in the Indo‐Pacific. In this study, we acquired the second generation of wild P. argenteus by artificial breeding and further studied the testicular development and ultrastructure of spermatogenesis. The results of gonadosomatic index (GSI) showed the spawning period of this marine fish was from April to June. Besides, through morphological analysis, we found that P. argenteus had an anastomosing tubular testis surrounded by a layer of tunica albuginea, in which spermatogenesis occurred in cysts where the synchronous germ cells were completely surrounded by the cytoplasmic projection of Sertoli cells. Meanwhile, based on submicroscopic characteristics, the germ cells are classified into nine different types. During the ontogenesis of testis, both the early stage of spermatogenesis and sperm were observed in P. argenteus. At sperm maturation stage, different types of spermatozoa and activation of sperms occurred non‐synchronously in the tubules. Cytoplasmic bridges also were observed among synchronous germ cells within the cysts, suggesting an interrelated and differentiated relationship among these germ cells.

Production of sterile mono-sex triploid yellow drum (Nibea albiflora): genotypic females and sex-reversed phenotypic males with emphasis on utilization as surrogate broodstock

“…However, only small amounts of SZ-like cells were produced in the lobules in triploid testes during the first spawning season. Compared with ultrastructural features of normal spermatogenesis in fish species (Yang et al 2021a , b ; Liu et al 2021 ; Papah et al 2013 ), triploid male yellow drum also had seven types of male germ cells including SPG, five types of SCs and SZ. However, a large number abnormal anaphase/telophase II SCs and ST varied in size and DSs were also observed in triploid testes.…”

“…Histologically, although several scattered PNOs were observed, the ovaries of triploids were composed of a large number of CNOs. In teleosts, ultrastructural features of normal oogenesis are similar, the germ cells can be developed from oogonia into mature eggs in Bluefin Tuna ( Thunnus thynnus ) (Abascal and Medina 2005 ) and silver pomfret ( Pampus argenteus ) (Yang et al 2021a , b ). In this study, ultrastructure of germ cells showed only three types of germ cells including the OG, CNOs, and PNOs existed in triploid ovaries.…”

Whole-genome survey reveals interspecific differences in genomic characteristics and evolution of Pampus fish

“…Additionally, P. argenteus and P. echinogaster exhibit remarkable morphological similarities, prompting Liu and Li (2013b) to meticulously delineate the distinguishing characteristics of the former species. Recently published studies have predominantly focused on P. argenteus, encompassing investigations into its early developmental characteristics, biological properties, and gene functionality, among other aspects (Gao et al, 2020;Al-Abdul-Elah et al, 2021;Wang et al, 2021;Yang et al, 2021a;Yang et al, 2021b). Researchers have employed diverse molecular markers to investigate disparities among Pampus species.…”

Insulin-like growth factor 1 promotes the gonadal development of Pampus argenteus by regulating energy metabolism

“…As a result, most of the body's energy is used for reproductive processes. Under artificial breeding conditions, we discovered instances of ovarian atresia and asynchronous sperm development in some silver pomfret specimens [33, 34]. Additionally, due to generations of breeding, the gonad development of silver pomfret in artificial culture has been accelerated.…”