Morphological Evaluation of Oxidative Phosphorylation System in Myocardial Infarction under Conditions of Modified Vascular Endothelial Growth Factor Concentration

Шурыгин, М. Г.; Shurygina, I. A.; Kanya, O. V.; Дремина, Н. Н.; Лушникова, Е. Л.; Nepomnyashchikh, R. D.

doi:10.1007/s10517-015-2974-x

Cited by 5 publications

(4 citation statements)

References 10 publications

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“…Alexa fluor 568 goat anti-mouse IgG (H + L) or Alexa fluor 488 goat anti-rabbit IgG (H + L) in a dilution of 1:300 was used as secondary antibody. Nuclei were stained with Hoechst, 1:300 [ 55 , 56 ]. The fluorescent stain intensity for each preparation was measured using the Nis-elements software (Nikon Corporation, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Safety and Toxicity of the Original Copper Nanocomposite Based on Poly-N-vinylimidazole

et al. 2021

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A new original copper nanocomposite based on poly-N-vinylimidazole was synthesized and characterized by a complex of modern physicochemical and biological methods. The low cytotoxicity of the copper nanocomposite in relation to the cultured hepatocyte cells was found. The possibility to involve the copper from the nanocomposite in the functioning of the copper-dependent enzyme systems was evaluated during the incubation of the hepatocyte culture with this nanocomposite introduced to the nutrient medium. The synthesized new water-soluble copper-containing nanocomposite is promising for biotechnological and biomedical research as a new non-toxic hydrophilic preparation that is allowed to regulate the work of key enzymes involved in energy metabolism and antioxidant protection as well as potentially serving as an additional source of copper.

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Section: Methodsmentioning

confidence: 99%

Evaluation of the Safety and Toxicity of the Original Copper Nanocomposite Based on Poly-N-vinylimidazole

et al. 2021

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“…(31) Sections were prepared and stained by the immunofluorescence method. (32)(33)(34) Antibodies to Ki-67 rabbit polyclonal (Abbiotec, Cat. N 250733, Lot 09092202) in a dilution of 1:300 were used as primary antibodies; Alexa fluor 568 goat anti-rabbit IgG (H+L) (Invitrogen, Cat.…”

Section: Methodsmentioning

confidence: 99%

Using Ki-67 Mitotic Activity Markers as a Predictor of the Progression of Adhesions in the Abdominal Cavity

Shurygina¹,

Шурыгин²,

Chepurnykh³

et al. 2020

IJBM

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Background: Ki-67 is a nuclear protein expressed in all proliferating cells of vertebrates during mitotic cycle phases S, G1, G2, and M, except for G0. Studying this marker is widely used to diagnose the proliferative activity of tumors. However, studying Ki-67 in non-neoplastic diseases attracts much less attention among the researchers. The aim of this study was to assess the possibility of using staining for Ki-67 to identify the proliferative potential of fibroblasts during the formation of adhesions in the abdominal cavity (AC). Methods and Results: Experiments were carried out on male Wistar rats. The adhesion process in AC was simulated in the control group (n=25), and in the experimental group (n=25) with the administration of Seroguard®. Animals were sacrificed on Days 1–30, and the severity of the adhesive process in AC was assessed. Histological sections were prepared and stained for Ki-67. It was found that the animals of the control group had increased severity of the adhesive process in AC during the observation. Maximum increase in severity was registered on Day 30 – 12[9-13] points in the control group and 4[4-4] points in the experimental group (P=0.0079). High proliferative activity of fibroblasts in the control group was detected on Days 3, 7, 14 and 30, which may indicate an active division of fibroblasts and the formation of adhesions in the damaged area. In the experimental group, single Ki-67 positive cells were noted during the entire observation period, which may point to a reduced potential for the formation of adhesions. Conclusion: Our study showed the prospects of using Ki-67 staining to determine the severity of the developing adhesive process in AC, and also revealed one of the possible mechanisms that inhibit the formation of the adhesive process when using Seroguard® – a decrease in the mitotic activity of fibroblasts in the area of peritoneal injury.

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“…Значення СЕФР у патогенезі гострого інфаркту міокарда відзначають і інші автори [11,18,19]. Ендогенний СЕФР є аутокринним регуляторно-ростовим показником, оскільки сприяє активності культивованих клітин.…”

Section: результати та їх обговоренняunclassified

Physiological Significance of Vascular Endothelial Growth Factor in Patients With Acute Forms of Coronary Artery Disease

Гавриленко¹,

Ryzhkova²,

Parkhomenko³

et al. 2019

Fiziol Zh

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Morphological Evaluation of Oxidative Phosphorylation System in Myocardial Infarction under Conditions of Modified Vascular Endothelial Growth Factor Concentration

Cited by 5 publications

References 10 publications

Evaluation of the Safety and Toxicity of the Original Copper Nanocomposite Based on Poly-N-vinylimidazole

Evaluation of the Safety and Toxicity of the Original Copper Nanocomposite Based on Poly-N-vinylimidazole

Using Ki-67 Mitotic Activity Markers as a Predictor of the Progression of Adhesions in the Abdominal Cavity

Physiological Significance of Vascular Endothelial Growth Factor in Patients With Acute Forms of Coronary Artery Disease

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