Whole‐body vibration has been considered as a countermeasure against muscle atrophy. However, its effects on muscle atrophy are poorly understood. We evaluated the effects of whole‐body vibration on denervated skeletal muscle atrophy. Whole‐body vibration was performed on rats from Day 15 to 28 after denervation injury. Motor performance was evaluated using an inclined‐plane test. Compound muscle action potentials of the tibial nerve were examined. Muscle wet weight and muscle fiber cross‐sectional area were measured. Myosin heavy chain isoforms were analyzed in both muscle homogenates and single myofibers. Whole‐body vibration resulted in a significantly decreased inclination angle and muscle weight, but not muscle fiber cross‐sectional area of fast‐twitch gastrocnemius compared to denervation only. In denervated gastrocnemius, a fast‐to‐slow shift was observed in myosin heavy chain isoform composition following whole‐body vibration. There were no significant changes in muscle weight, muscle fiber cross‐sectional area, and myosin heavy chain isoform composition in denervated slow‐twitch soleus. These results imply that whole‐body vibration does not promote recovery of denervation‐induced muscle atrophy.