Morphological characterization of the growing front of Rhizopus oligosporus in solid media

Díaz, Brenda Hildeliza Camacho; Aparicio, Antonio Jiménez; Chanona-Pérez, Jorge; Calderón‐Domínguez, Georgina; Alamilla-Beltrán, Liliana; Hernández‐Sánchez, Humberto; Gutiérrez-López, Gustavo F.

doi:10.1016/j.jfoodeng.2010.06.028

Cited by 11 publications

(4 citation statements)

References 37 publications

(50 reference statements)

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“…This finding was evidently related to the physical characteristics of the culture medium, with compact and viscous culture medium resulting in difficult mycelial growth. The results obtained through this research were consistent with those attained by other authors [19,30,40].…”

Section: Rhizopus Growth In Different Fluffiness Substrate and Glucoasupporting

confidence: 92%

Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

Tang¹,

Luo²,

Xue³

et al. 2018

Journal of Microbiology and Biotechnology

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Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, µ of mycelium reached to 0.15 h − 1 after 45 h of culture in a thermostatic water bath incubator at 30°C. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.

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Section: Rhizopus Growth In Different Fluffiness Substrate and Glucoasupporting

confidence: 92%

Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

Tang¹,

Luo²,

Xue³

et al. 2018

Journal of Microbiology and Biotechnology

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show abstract

“…Matlab and fractal dimensions were used for mycelium image transformation and analysis in that study 28 . Díaz et al 29 have also reported the same type of culture condition for characterizing macro and micro structural development of Rhizopus oligosporus NRRL-2710 colonies growing on solid media in Petri dishes through image processing and fractal dimension. They found that growth of the colony front was useful for evaluating parameters of fungal development such as the number of tips and the average hypha length 29 .…”

Section: Discussionmentioning

confidence: 99%

Rapid and concise quantification of mycelial growth by microscopic image intensity model and application to mass cultivation of fungi

Lee

Kim

et al. 2021

Sci Rep

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The microbial food fermentation industry requires real-time monitoring and accurate quantification of cells. However, filamentous fungi are difficult to quantify as they have complex cell types such as pellet, spores, and dispersed hyphae. In this study, numerous data of microscopic image intensity (MII) were used to develop a simple and accurate quantification method of Cordyceps mycelium. The dry cell weight (DCW) of the sample collected during the fermentation was measured. In addition, the intensity values were obtained through the ImageJ program after converting the microscopic images. The prediction model obtained by analyzing the correlation between MII and DCW was evaluated through a simple linear regression method and found to be statistically significant (R2 = 0.941, p < 0.001). In addition, validation with randomly selected samples showed significant accuracy, thus, this model is expected to be used as a valuable tool for predicting and quantifying fungal growth in various industries.

show abstract

Section: Discussionmentioning

confidence: 99%

Rapid and Concise Quantification of Mycelial Growth by Microscopic Image Intensity Model and Application to Mass Cultivation of Fungi

Lee¹,

Lee²,

Kim³

et al. 2021

Preprint

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The microbial food fermentation industry requires real-time monitoring and accurate quantification of cells. However, filamentous fungi are difficult to quantify as they have complex cell types such as pellet, spores, and dispersed hyphae. In this study, numerous data of microscopic image intensity (MII) were used to develop a simple and accurate quantification method of Cordyceps mycelium. The dry cell weight (DCW) of the sample collected during the fermentation was measured. In addition, the intensity values were obtained through the ImageJ program after converting the microscopic images. The prediction model obtained by analyzing the correlation between MII and DCW was evaluated through a simple linear regression method and found to be statistically significant (R2 = 0.941, p <0.001). In addition, validation with randomly selected samples showed significant accuracy, thus, this model is expected to be used as a valuable tool for predicting and quantifying fungal growth in various industries.

show abstract

Morphological characterization of the growing front of Rhizopus oligosporus in solid media

Cited by 11 publications

References 37 publications

Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

Rapid and concise quantification of mycelial growth by microscopic image intensity model and application to mass cultivation of fungi

Rapid and Concise Quantification of Mycelial Growth by Microscopic Image Intensity Model and Application to Mass Cultivation of Fungi

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