Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

Abstract

Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, µ of mycelium reached to 0.15 h − 1 after 45 h of culture in a thermostatic water bath incubator at 30°C. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.

Production of Glucoamylase from Novel Strain of Alternaria Alternata under Solid State Fermentation

“…Potato culture media proved to be the best substrate for glucoamylase production for the growth of Rhizopus oryzae . So, the results revealed that the use of potato as diffusion juices with agar and sugar or with wheat bran was applied in biotechnology field for the growth of various fungal strains [ 23 ], but the maximum yield of glucoamylase by using dried potato powder as a fermentation media under solid state fermentation is a novel phenomenon in which Alternaria alternata was grown on this solid substrate.…”

Analysis of Saccharification Products of High-Concentration Glutinous Rice Fermentation by Rhizopus nigricans Q3 and Alcoholic Fermentation of Saccharomyces cerevisiae GY-1

“…All pots were covered, marked, and then sterilized for 45 min in a 118 °C pressure steam autoclave. Next, the mixtures were cooled, and a 10% (mass fraction of glutinous rice) R. nigricans Q3 seed solution (3,6,9,12,15,18,21, and 24 mL) was inoculated. Glutinous rice and R. nigricans Q3 were mixed completely, and the mixtures were sealed with a plastic film.…”

“…Rhizopus can grow in culture media with low water content because it simultaneously undergoes growth and saccharification. 11,12 It is used to prepare rice liquor and syrup 13 via solid-state and semisolid-state methods. Moreover, Saccharomyces cerevisiae can also produce a series of zymases to hydrolyze micromolecular monosaccharides into alcohol.…”

“…niger, and Absidia orchidis for saccharification. , Rhizopus can secrete a large amount of amylase and glucoamylase for hydrolyzing starch polysaccharides. , Rhizopus can cut α-1,4 and α-1,6 bonds in raw starch, thereby transforming starch into fermentable sugar. Rhizopus can grow in culture media with low water content because it simultaneously undergoes growth and saccharification. , It is used to prepare rice liquor and syrup via solid-state and semi-solid-state methods. Moreover, Saccharomyces cerevisiae can also produce a series of zymases to hydrolyze micromolecular monosaccharides into alcohol. , …”