Morphological characterization, cytogenetic analysis, and geographical distribution of the Pygmy Marbled Newt Triturus marmoratus pygmaeus (Wolterstorff, 1905) (Caudata: Salamandridae)

Garcı́a-Parı́s, Mario; Herrero, Pilar; Martín, Carlos A.; Dorda, Jesús Dorda; Esteban, Marisa; Arano, B.

doi:10.1163/26660644-06301001

Cited by 16 publications

(11 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In species with a wide geographic distribution of the samples (e.g. T. marmoratus), the standard deviation is higher than for samples from a single locality, perhaps re ecting geographic variation, as the populations belong probably to different subspecies: T. marmoratus pygmaeus from Algarve (Portugal) and T. marmoratus marmoratus from El Berrueco (Madrid, Spain) (García-París et al, 1993;Barbadillo et al, 1999).…”

Section: Resultsmentioning

confidence: 99%

Determination of cellular DNA content of Iberian salamanders by flow cytometry

et al. 2000

View full text Add to dashboard Cite

The DNA content per diploid nucleus (2 C-value) was determined for the eight species of Iberian salamanders by flow cytometry. All species showed high values, which are characteristic of Caudata. The species with the lowest value was Pleurodeles waltl (48.25 pg DNA/cell) and Salamandra salamandra had the highest values (70.55 pg DNA/cell). There were no statistical differences between sexes in any of the eight species and DNA content was not directly related with phylogeny. The values obtained are often higher than previously published estimates obtained with older and potentially less accurate methodologies. We discuss the possible relationship between cellular DNA content and reproductive biology. Se determinó el contenido en ADN por núcleo diploide (valor 2-C) por citometría de flujo de ocho especies de salamandras ibéricas. Todas las especies mostraron altos valores, lo que es característico de Caudata. La especie con el menor valor fue Pleurodeles waltl (48,25 pg ADN/célula) mientras Salamandra salamandra tuvo las valores más altos (70,55 pg ADN/célula). No se encontraron diferencias estadísticas entre sexos en ninguna de las 8 especies y el contenido en ADN no se relacionó directamente con la filogenia. Los valores obtenidos son, a menudo, más altos que los publicados previamente con técnicas más antigüas, y potencialmente menos precisas. Discutimos las posibles relaciones entre el contenido en ADN celular y la biología reproductiva de cada especie.

show abstract

Section: Resultsmentioning

confidence: 99%

Determination of cellular DNA content of Iberian salamanders by flow cytometry

et al. 2000

View full text Add to dashboard Cite

show abstract

“…Triturus marmoratus and T. pygmaeus are similar, yet morphologically diagnosable species (García-París et al, 1993;Arntzen, 2018). They are genetically distinct (Espregueira Themudo and Arntzen, 2007b;Wielstra et al, 2019), with genetic admixture reported over several sections of their parapatric species border (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

A genomic footprint of a moving hybrid zone in marbled newts

Arntzen

López-Delgado

Riemsdijk

et al. 2020

Preprint

View full text Add to dashboard Cite

We developed a panel of 44 nuclear genetic markers and applied this to two species of marbled newts in the north (Triturus marmoratus) and the south (T. pygmaeus) of the Iberian Peninsula, to document pattern and process of interspecific gene flow. The northernmost occurrence of T. pygmaeus genetic material was in a T. marmoratus population north of the Vouga river estuary. This suggested the past presence of a hybrid zone, possibly coinciding with a natural river outlet at ca. 1200 A.D. Since 1808, the species contact has moved back south to a by then completed, man-made Vouga channel. We also found evidence for a T. marmoratus genomic footprint in T. pygmaeus from the Serra de Sintra, near Lisbon. In combination with a previously reported southern, relic occurrence of T. marmoratus in between both areas, the data point to the superseding with hybridization of T. marmoratus by T.pygmaeus. We estimate that the species hybrid zone has moved over a distance of ca. 215 km.

show abstract

“…Adult and juvenile newts were caught by dip netting across Europe and Asiatic Turkey. Individuals were identifi ed to species based on morphology (García-París et al, 1993;Arntzen and Wallis, 1999). For phylogeny reconstruction, two to four populations per species were selected from a larger sample set, such that 1) identifi cations are in line with documented geographical distributions, 2) spatial coverage is large, and 3) samples from putative admixture zones are preferably excluded (Fig.…”

Section: Sampling Strategymentioning

confidence: 99%

The phylogeny of crested newts (Triturus cristatus superspecies): nuclear and mitochondrial genetic characters suggest a hard polytomy, in line with the paleogeography of the centre of origin

Arntzen

Themudo

Wielstra

2007

CTOZ

View full text Add to dashboard Cite

Newts of the genus Triturus (Amphibia, Caudata, Salamandridae) are distributed across Europe and adjacent Asia. In spite of its prominence as a model system for evolutionary research, the phylogeny of Triturus has remained incompletely solved. Our aim was to rectify this situation, to which we employed nuclear encoded proteins (40 loci) and mitochondrial DNA-sequence data (mtDNA, 642 bp of the ND4 gene). We sampled up to four populations per species covering large parts of their ranges. Allozyme and mtDNA data were analyzed separately with parsimony, distance, likelihood and Bayesian methods of phylogenetic inference. Existing knowledge on taxonomic relationships was confi rmed, including the monophyly of the genus and the groups of crested newts (four species) and marbled newts (two species). The genetic coherence of species and subspecies was also confi rmed, but not always with high statistical support (depending on taxon, characters under consideration, and method of inference). In spite of our efforts we did not obtain suffi cient phylogenetic signal to prefer one out of twelve potential topologies representing crested newts relationships. We hypothesize that the lack of phylogenetic resolution refl ects a hard polytomy and represents the (near)-simultaneous origin of crested newt species. Using a calibration point of 24 Ma (million years before present) for the most recent common ancestor of Triturus-species, the crested newt radiation event is dated at 7-6 Ma (scenario 1) or at 11-10 Ma (scenario 2), depending on the application of an allozyme versus mtDNA molecular clock. The fi rst biogeographical scenario involves the spread of crested newts from the central Balkans into four compass directions. This scenario cannot be brought into line with potential vicariance events for south-eastern Europe. The second scenario involves the more or less simultaneous origin of four species of crested newts through large-scale vicariance events and is supported by the paleogeographical reconstruction for the region at the end of the Middle Miocene. The subspecies Triturus carnifex macedonicus carries in one large area the mtDNA that is typical for the neighbouring species T. karelinii, which is attributed to introgression and a recent range shift. It is nevertheless a long distinct evolu-tionary lineage and we propose to elevate its taxonomic status to that of a species, i.e., from Triturus c. macedonicus (Karaman, 1922) to Triturus macedonicus (Karaman, 1922).

show abstract

Morphological characterization, cytogenetic analysis, and geographical distribution of the Pygmy Marbled Newt Triturus marmoratus pygmaeus (Wolterstorff, 1905) (Caudata: Salamandridae)

Cited by 16 publications

References 3 publications

Determination of cellular DNA content of Iberian salamanders by flow cytometry

Determination of cellular DNA content of Iberian salamanders by flow cytometry

A genomic footprint of a moving hybrid zone in marbled newts

The phylogeny of crested newts (Triturus cristatus superspecies): nuclear and mitochondrial genetic characters suggest a hard polytomy, in line with the paleogeography of the centre of origin

Contact Info

Product

Resources

About