Hybridization has many and varied impacts on the process of speciation. Hybridization may slow or reverse differentiation by allowing gene flow and recombination. It may accelerate speciation via adaptive introgression or cause near-instantaneous speciation by allopolyploidization. It may have multiple effects at different stages and in different spatial contexts within a single speciation event. We offer a perspective on the context and evolutionary significance of hybridization during speciation, highlighting issues of current interest and debate. In secondary contact zones, it is uncertain if barriers to gene flow will be strengthened or broken down due to recombination and gene flow. Theory and empirical evidence suggest the latter is more likely, except within and around strongly selected genomic regions. Hybridization may contribute to speciation through the formation of new hybrid taxa, whereas introgression of a few loci may promote adaptive divergence and so facilitate speciation. Gene regulatory networks, epigenetic effects and the evolution of selfish genetic material in the genome suggest that the Dobzhansky-Muller model of hybrid incompatibilities requires a broader interpretation. Finally, although the incidence of reinforcement remains uncertain, this and other interactions in areas of sympatry may have knock-on effects on speciation both within and outside regions of hybridization.
Significance Snake venoms are toxic protein cocktails used for prey capture. To investigate the evolution of these complex biological weapon systems, we sequenced the genome of a venomous snake, the king cobra, and assessed the composition of venom gland expressed genes, small RNAs, and secreted venom proteins. We show that regulatory components of the venom secretory system may have evolved from a pancreatic origin and that venom toxin genes were co-opted by distinct genomic mechanisms. After co-option, toxin genes important for prey capture have massively expanded by gene duplication and evolved under positive selection, resulting in protein neofunctionalization. This diverse and dramatic venom-related genomic response seemingly occurs in response to a coevolutionary arms race between venomous snakes and their prey.
The newts Triturus vulgaris and Triturus montandoni are sister species that exhibit contrasting levels of intraspecific morphological variation. Triturus vulgaris has a broad Eurasiatic distribution encompassing both formerly glaciated and unglaciated areas and shows substantial morphological differentiation in the southern part of its range, while T. montandoni, confined to the Carpathians, is morphologically uniform. We analysed sequence variation of two mtDNA fragments of the total length of c. 1850 bp in 285 individuals of both species collected from 103 localities. Phylogenetic analysis of 200 unique haplotypes defined 12 major clades, their age estimated at c. 4.5-1.0 million years (Myr). Most of the older clades were found in the southern part of the range, and also in central Europe, mainly in Romania. The distribution of mtDNA clades points to the existence of several glacial refugia, located in the Caucasus region, Anatolia, the Balkan Peninsula, Italy, and more to the north in central Europe. The concordance between mtDNA based phylogeny and the distribution of T. vulgaris subspecies was weak. Triturus montandoni haplotypes did not form a monophyletic group. Instead they were found in six clades, in five of them mixed with T. vulgaris haplotypes, most likely as a result of past or ongoing hybridization and multiple introgression of mtDNA from T. vulgaris to T. montandoni. Patterns of sequence variation within clades suggested long-term demographic stability in the southern groups, moderate and relatively old demographic growth in the populations inhabiting central Europe, and high growth in some of the groups that colonized northern parts of Europe after the last glacial maximum.
Two hybridizing species of newts, Triturus cristatus and T. marmoratus, with overlapping distributions show a parapatric distribution when surveyed in detail. The factors that govern the distribution of cristatus vs. marmoratus in the département (province) of Mayenne in western France are identified as forestation and relief. The parapatric hybrid zone running through Mayenne is narrow but widens to approximately 20 km in an area with mixed habitat. In this area most breeding sites are shared and F hybrids form about 4% of the total population. Analysis of survey data collected about 30 years previously also shows an essentially parapatric distribution. Comparison of past and present distribution maps reveals that cristatus has superseded marmoratus over large areas in the south of Mayenne. An area where marmoratus replaced cristatus also exists, but it is more limited in size. Gene flow between cristatus and marmoratus is analyzed using 10 diagnostic genetic markers [9 protein loci and mitochondrial (mt) DNA]. In syntopic populations nuclear gene flow is bidirectional with a mean frequency of introgressed alleles (f) of 0.3%. In allotopic populations of cristatus and marmoratus gene flow is present in areas of species replacement (f = 0.3%), while gene flow appears to be absent in those areas that have been continuously occupied by a single species. At the biogeographic level, the presence or absence of introgression is paralleled by the persistence or absence, respectively, of pockets of cristatus-marmoratus syntopy. All F hybrids possess the cristatus type mtDNA. This may be due to asymmetric interspecific mate choice and would explain the observed absence of introgression of the maternally inherited mtDNA genome in areas where cristatus replaced marmoratus. The cristatus-marmoratus hybrid zone bears characteristics of both the clinal (parapatric) hybrid zone model and the mosaic hybrid zone model. Such a mixed model-for which we propose the term "reticulate hybrid zone"-can be appreciated only if studied over a two-dimensional geographic area and also through time.
The golden-striped salamander (Chioglossa lusitanica) is an ecologically specialized species, endemic to north-western Iberia. Patterns of genetic variation were assessed at seven polymorphic enzyme loci and one mitochondrial DNA (mtDNA) marker (cytochrome b) in 17 populations across its range. Estimates of enzyme genetic diversity revealed a high degree of genetic subdivision (FST = 0.68), mainly attributable to the existence of two groups of populations. The groups were located, respectively, north and south of the Mondego River, indicating that this river coincided with a major historical barrier to gene flow. A significant decrease in genetic variability from the Mondego northwards was associated with the Douro and Minho rivers. mtDNA sequence variation revealed a congruent pattern of two haplotype groups (d = 2.2%), with a geographical distribution resembling that of allozymes. The pattern and depth of genetic variation is consistent with the following hypotheses: (i) subdivision of an ancestral range of the species prior to the middle Pleistocene; (ii) secondary contact between populations representing historical refugia; (iii) relatively recent range expansion giving rise to the northern part of the species range; and (iv) loss of genetic variation through founder effects during range expansion across major rivers.
The monophyly of European newts of the genus Triturus within the family Salamandridae has for decades rested on presumably homologous behavioral and morphological characters. Molecular data challenge this hypothesis, but the phylogenetic position of Triturus within the Salamandridae has not yet been convincingly resolved. We addressed this issue and the temporal divergence of Triturus within the Salamandridae with novel Bayesian approaches applied to DNA sequence data from three mitochondrial genes (12S, 16S and cytb). We included 38 salamandrid species comprising all 13 recognized species of Triturus and 16 out of 17 salamandrid genera. A clade comprising all the ''Newts'' can be separated from the ''True Salamanders'' and Salamandrina clades. Within the ''Newts'' well-supported clades are: Tylototriton-Pleurodeles, the ''New World Newts'' (Notophthalmus-Taricha), and the ''Modern Eurasian Newts'' (Cynops, Pachytriton, Paramesotriton 5 together the ''Modern Asian Newts'', Calotriton, Euproctus, Neurergus and Triturus species). We found that Triturus is a non-monophyletic species assemblage, which includes four groups that are themselves monophyletic: (i) the ''Large-Bodied Triturus'' (six species), (ii) the ''Small-Bodied Triturus'' (five species), (iii) T. alpestris and (iv) T. vittatus. We estimated that the last common ancestor of Triturus existed around 64 million years ago (mya) while the root of the Salamandridae dates back to 95 mya. This was estimated using a fossil-based molecular dating approach and an explicit framework to select calibration points that least underestimated their corresponding nodes. Using the molecular phylogeny we mapped the evolution of life history and courtship traits in Triturus and found that several Triturus-specific courtship traits evolved independently. J. Exp. Zool. (Mol. Dev. Evol.) 308B:139-162, 2007. r 2006 How to cite this article: Steinfartz S, Vicario S, Arntzen JW, Caccone A. 2007. A Bayesian approach on molecules and behavior: reconsidering phylogenetic and evolutionary patterns of the salamandridae with emphasis on Triturus newts. J. Exp. Zool. (Mol. Dev. Evol.) 308B:139-162.Salamanders and newts of the family Salamandridae are at present distributed over North America and Eurasia. Despite a profound diversity in life history traits, including courtship and reproductive modes, the monophyly of the Salamandridae is strongly supported (Weisrock et al., 2005). Among the 17 extant salamandrid genera, the genus Triturus has the widest distribution and the highest number of species, since it is found throughout Europe except for northern Scandina- 308B:139-162 (2007) via and the Mediterranean islands (Fig. 1). The monophyly of Triturus was generally assumed on the basis of overall similarity in morphology and traits of courtship behavior. Triturus newts display a biphasic life style with aquatic reproduction, typically taking place in ponds and stagnant waters during spring and early summer, and a subsequent terrestrial phase until the next reproductive eve...
The crested newt has a widespread European distribution and encompasses four taxa recently elevated to full species: Triturus cristatus, T. carnifex, T. dobrogicus, and T. karelini. These are distinct on morphological, chromosomal, and isozymic grounds and have fairly sharp transition zones. A widespread survey (12 countries, 49 geographic sites, 210 individuals) of mtDNA variation (20-27 restriction enzyme sites mapped per individual) was made in order to 1) correlate mtDNA variation with morphological features defining the species, 2) determine the degree of differentiation within and among species, and 3) detect any introgression among species. The mtDNAs of these species were clearly differentiated (d = 3.9-7.1%). Additionally, geographic structuring was observed within T. carnifex and T. karelini, each displaying two divergent mitochondrial genome types (d = 3.5% and 4.7%, respectively). The other two (more northerly distributed) species were genetically homogeneous over most (T. cristatus) or all (T. dobrogicus) of their ranges. In the case of T. cristatus, one may infer bottlenecking as a result of Pleistocene glaciation events. This may also apply in part to T. dobrogicus, but high population connectedness and gene flow in this lowland river species may alone be sufficient for homogenization of mtDNA. Patterns of mtDNA variation were largely concordant with morphology; some interspecific mitochondrial gene flow was observed, but only close to or in the transition zones. Analyses of mapped restriction-site data by UPGMA and parsimony methods (using the closely related T. marmoratus as an outgroup) produce very similar dendrograms. The levels of divergence found concur with the systematics of the group, but the differentiation within T. carnifex and T. karelini is notable.
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