Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes

Abstract

Hidayati NR, Suranto, Sajidan. 2018. Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes. Biodiversitas 19: 1683-1689. Aims of this research were to investigate the morphological character and isozyme banding patterns of Cucurbita moschata plants grown at three different altitudes. Samples in this study consisted of leaf, stem, and flowers. The morphological characters were conducted by direct observation in the field and analyzed descriptively as well as statically by one way ANOVA. The isozyme bands appearance of esterase and peroxidase of leaf samples were conducted using polyacrylamide gel electrophoresis (PAGE). Qualitative approach was used to analyze the presence and the absence of isozyme bands, while Retardation factor (Rf) was used to analyze quantitatively. The results showed that most plants grown at middle altitude (351-750 m asl.) were well-developed in terms of length of leaves, stems and flowers. Accordingly, the isozyme banding pattern of peroxidase was also found varied in plants grown at middle altitudes from which the presence of very unique bands was detected. Conversely, the band detected in plants grown at the lower and the highest altitudes was similar in term of band's number but it was different in the quality of the bands. Meanwhile, esterase isozyme banding pattern of plants grown at the lower and higher altitude had more bands than the middle altitude. Based on this result it is obvious that the isozyme data could be used to support in understanding the diversity morphological characters of plants grown in three different altitudes. This early result suggests that altitudes as a crucial factor in contributing the expression of isozyme appearance, which is useful for further pumpkin characterizations.

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

“…Recent studies dealing with peroxidase isozyme had been conducted by Suranto et al (2017), who noted that not only the total number of isozyme bands was useful in distinguishing the difference between the healthy and the virus-infected plants, but this enzyme activity on both samples was also able to compare, especially through their band thickness, between the healthy and the diseased plants. Other interesting results of the above two isozymes were reported by Hidayati et al (2018) dealing with Cucurbita moschata at different altitudes. They note that 10 of peroxidase isozymes and 8 of esterase isozymes were recorded.…”

“…It is considered that these isozymes data were recorded to be one of the most often used in conjunction with group of plant differentiation among taro accession (Smith and Balamarugar (2014) and Kumar et al 2015). Similar studies using the same two enzyme systems were also conducted by Hidayati et al (2018) employing the leaf samples of Cucurbita moschata. They recorded 10 bands of peroxidase isozyme and 8 isozymes banding pattern of esterase.…”

Trace Elements in Edible Flowers from Italy: Further Insights into Health Benefits and Risks to Consumers

“…The floral species analyzed are listed in Table S1 together with their main botanical characteristics and the related references therein [ 7 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ...…”

Population study of Cucurbita moschata based on morphological characters and isozyme banding patterns in Bima District, West Nusa Tenggara, Indonesia

“…Gong et al (2018) reported that changing the environmental conditions could cause plants to adapt both morphologically and physiologically. These adaptation processes have the potential to result in both qualitative and quantitative variations in the plant's morphological characters (Hidayati et al 2018). According to Muthumani et al (2013), altitude influences the degree of light intensity, temperature, rainfall, relative humidity, as well as wind velocity.…”

“…Pumpkin has several varieties of usefulness for human beings, not only as a staple food, but also for the diet of people with diabetes millitus (DM). This usefulness is related to the low carbohydrate content and it is easy to set (Suranto et al 2015;Hidayati et al 2018). According to Nagar et al (2018) and Quintana et al (2018), the genotype of pumpkins resulted in their different mineral nutrients, in which the highest percentage of carbohydrate content was detected from the pulps.…”