Morphological Changes in the Parathyroid Gland of Rats with Humoral Hypercalcaemia of Malignancy

Nakanishi, Masayoshi; Sawamoto, Osamu; Kawashima, Maki; Kuwamura, Mitsuru; Yamate, Jyoji

doi:10.1016/j.jcpa.2004.01.002

Cited by 5 publications

(6 citation statements)

References 11 publications

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“…However, at young ages, parathyroid gland of Guinea pigs only contained chief cells which appeared as irregular clusters, cords and follicles around blood capillaries. These findings were in line with those reported for most rodents (Nakanishi et al, 2004) as well as human (Chen et al, 2013;Mini and Manju, 2017).…”

Section: Caspase 3 and Baxsupporting

confidence: 92%

Electron Microscopy and Immunohistochemistry using Indicators of Apoptosis and Proliferation on Guinea Pig Parathyroid Gland

Tantawy¹,

Elzoghby²,

Attia³

et al. 2021

AAVS

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Maintenance of blood calcium level depended on the parathyroid hormone secretion that released from the parathyroid gland which important for bone mineralization. This work aimed to investigate the ultrastructure and immunohistochemical reaction of parathyroid gland of Guinea pig. The study was carried out on twenty five healthy male Guinea pigs, sixty days prenatal, one month postnatal and twelve months postnatal. Parathyroid gland consisted of fibrous capsule and cellular parenchyma which included chief and oxyphil cells. Ultrastructuraly, parathyroid gland contained several chief cells, fibroblast and blood capillaries. Chief cells included two types; granulated and vacuolated. The granulated cells contained several organelles and different sizes of secretory granules. The vacuolated cells contained few organelles and several vacuoles. The organelles involved mitochondria, Golgi complex, lysosome and different shapes of RER. Immunohistochemical studies were done using caspase3 and Bax as marker for apoptosis. Bax was responsible for activation of caspase3 via mitochondrial pathway of apoptosis. PCNA and Ki-67 were used as markers for cell proliferation. Ki-67 reacted during different stages of cell cycle and increased in S-phase. PCNA reacts mainly in S-phase of cell cycle, indicating condensation of DNA in the nucleus. Immunohistochemical staining with PCNA and Ki-67 shows negative and positive nuclear reaction at early life, then cells tend to increase their nuclear reaction with age. Caspase3 showed a faint cytoplasmic reaction at early life and that increased gradually with age. Bax showed a positive reaction in the cytoplasm and increased with age.

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Section: Caspase 3 and Baxsupporting

confidence: 92%

Electron Microscopy and Immunohistochemistry using Indicators of Apoptosis and Proliferation on Guinea Pig Parathyroid Gland

Tantawy¹,

Elzoghby²,

Attia³

et al. 2021

AAVS

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“…It was reported that in most mammals and lower species of animals the oxyphil cells are present in the adult, and contain glycogen particles and free ribosome, granular endoplasmic reticulum, Golgi apparatus and secretary granule which are poorly developed, suggesting that oxyphil cells of normal parathyroid do not have an active function in the biosynthesis of parathyroid hormone (Capen & Martin, 2003). In most mammals there is a higher oxidation and hydrolytic enzymes activity in the chief cells than in the oxyphil cells (Nakanishi et al, 2004). The functions of the oxyphil cells are not known (Banks, 1993;Singh, 2010).…”

Section: Discussionmentioning

confidence: 99%

Histological and histochemical studies of parathyroid gland in one-humped camel (<i>Camelus dromedarus</i>)

Abubakar¹,

Oladele²,

Adamu³

2018

Sokoto J. Vet. Sc.

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Thirty one-humped camels were grouped into three: juvenile (Group A), adult (Group B), and old ages (Group C), were used for the study. The camel parathyroid gland tissues were obtained from camels slaughtered at the Kano Metropolitan abattoir. The glands were prepared for standard histological study using haematoxylin and eosin (H and E) stains for light microscopy. For histochemical studies, Periodic Acid Schiff (PAS) special stain was used to demonstrate glycogen and glycoprotein. The results showed that the parathyroid glands are surrounded by a thin capsule of connective tissue. They have abundant stroma of connective tissue, with large smooth muscles all over. The tissue of the parathyroid gland consists of clusters of principal or chief cells. There are two different functional stages of the principal cell. The light principal cell has a large, pale nucleus and pale cytoplasm (described as inactive). The dark principal cell is a smaller cell, with a small, dark nucleus and a dark cytoplasm (active cell). The presence of oxyphil cells was observed. Histochemical studies showed that the tissue of the parathyroid gland in one-humped camel reacted negatively to the Periodic Acid Schiff (PAS) stain. The parathyroid gland consisted of densely packed chief cells (light and dark cells), oxyphil cells, and abundance of adipose cells in the adult male and female. These cells were absent in the younger group, which is generally identical to what is found, specifically, in cows, goats, and in humans, which signifies that one-humped camel can be introduced as an experimental research animal model for human parathyroid gland, in understanding the etiology and possible solution to human parathyroid diseases and osteoporosis. The study of parthyroid gland will enhance the provision of basic and clinical information on endocrinology and related research fields, such as animal breeding and biotechnology in one humped camel.

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“…ASAC is a malignant tumor which originates from apocrine glands of the anal sac [3,9,11,17] . Meuten et al categorized the tumors into 3 types as solid, tubular and rosette [17] .…”

Section: Discussionmentioning

confidence: 99%

“…These structures were accompanied by a thin stroma consisting of capillaries and connective tissue, tumor cells were undifferentiated, multilobulated and polygonal, pleomorphism and hyperchromatic nuclei and invasive to surrounding glandular tissues, the primary tumor most commonly appeared as a deep-seated, firm, nodular perianal mass ventrolateral to the anus and involving, or within close proximity to the anal sac. Tumors were unilateral and typically [1][2][3] cm in diameter (range, 0.5-3 cm). …”

Section: Micro and Macroscopic Findingsmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of an anal sac adenocarcinoma tumor in a Spitz dog

Javanbakht

Tavassoli

Sabbagh

et al. 2013

Asian Pacific Journal of Tropical Biomedicine

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Morphological Changes in the Parathyroid Gland of Rats with Humoral Hypercalcaemia of Malignancy

Cited by 5 publications

References 11 publications

Electron Microscopy and Immunohistochemistry using Indicators of Apoptosis and Proliferation on Guinea Pig Parathyroid Gland

Electron Microscopy and Immunohistochemistry using Indicators of Apoptosis and Proliferation on Guinea Pig Parathyroid Gland

Histological and histochemical studies of parathyroid gland in one-humped camel (<i>Camelus dromedarus</i>)

Evaluation of an anal sac adenocarcinoma tumor in a Spitz dog

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