Morphological Changes in Paraurethral Area after Introduction of Tissue Engineering Construct on the Basis of Adipose Tissue Stromal Cells

Perrotta

2017

BackgroundAdipose-derived stem cells are recognized as being an effective mesenchymal stem cell population with enormous potential in different fields of regenerative medicine and stem cell therapy. Although there is unanimous agreement on the harvesting procedure for adipose tissue, there are various protocols for adipose-derived stem cell isolation. The aim of this study was compare two methods of adipose-derived stem cells (ASCs) isolation, one based on a mechanical + enzymatic (ME) procedure and the other one exclusively mechanical (MC), in order to determine which one was superior to the other in accordance with current European and US legislation.MethodsWe reported step by step the two different methods ASCs isolation by comparing them. The ME procedure included the use of a centrifuge, an incubator and collagenase digestion solution (Collagenase NB 6 GMP Grade 17458; Serva GmbH, Heidelberg, Germany). The MC procedure was performed by vibrating shaker and centrifuge, both placed in a laminar airflow bench.ResultsWith the ME procedure, a mean of 9.06 × 105 ASCs (range, 8.4 to 9.72 × 105; SD ± 6.6 × 105) was collected, corresponding to 25.9% of the total number of harvested cells. With the MC procedure, a mean of 5 × 105 ASCs (range: 4.0 to 6.0 × 105; SD, ±1 × 105) was collected, corresponding to 5% of the total number of harvested cells.ConclusionBased on data collected, from the same amount of lipoaspirate the ME procedure allowed to isolate a greater number of ASCs (25,9%) compared to the MC one (5%).

Section: Introductionmentioning

confidence: 99%

Adipose-derived stem cells: Comparison between two methods of isolation for clinical applications

Raposio

Perrotta

2017

“…Fat is an active and dynamic tissue composed of several different cell types, including adipocytes, fibroblasts, smooth muscle cells, endothelial cells, and adipogenic progenitor cells called pre-adipocytes [30], [31], [32]. Stem cells isolated from lipoaspirates have demonstrated a broad in vitro adipogenic, chondrogenic, osteogenic, and myogenic lineage commitment [33], [34] as well as differentiation into pancreatic cells, hepatocytes, and neurogenic cells [35], [36], [37]. Cytometric analysis of adipose-derived stem cells (ASCs) has shown that these cells do not express CD31 and CD45, but do express CD34, CD73, CD105, and the mesenchymal stem cell marker CD90 [38], [39].…”

Section: Efficacy Of Lipofillingmentioning

confidence: 99%

Autologous fat transplantation for breast reconstruction: A literature review

Bertozzi

Grieco

et al. 2016

IntroductionThe use of autologous fat transplantation to correct volume and contour defects, scars, and asymmetry after breast cancer surgery has increased over the past 20 years. Many developments and refinements in this technique have taken place in recent years, and several studies of the safety of lipofilling in the breast have been published.Presentation of caseWe performed a literature review of this technique, highlighting the crucial role of lipofilling in breast cancer reconstruction.DiscussionThe efficacy of the fat graft transplantation depends on the experience and the technique used by the surgeon. The ASCs (adipose-derived stem cells) contained in the fat graft has proven to be crucial for breast reconstruction by mean the regeneration of tissue, through the chemotactic, paracrine, and immunomodulatory activities and their in situ differentiation.ConclusionThe role of lipofilling for breast reconstruction could be more significant with the application of the findings of experimental research on tissue engineering and ASCs.

“…Fat is an active and dynamic tissue composed of several different cell types, including adipocytes, fibroblasts, smooth muscle cells, endothelial cells, and adipogenic progenitor cells called pre-adipocytes [2] , [3] , [4] , [5] . Stem cells isolated from lipoaspirates have demonstrated broad in vitro adipogenic, chondrogenic, osteogenic, and myogenic lineage commitment [6] , [7] as well as the ability to differentiate into pancreatic cells, hepatocytes, and neurogenic cells [8] , [9] , [10] . Cytometric analysis of adipose-derived stem cells (ASCs) has shown that these cells do not express CD31 and CD45, but express CD34, CD73, CD105, and the mesenchymal stem cell marker CD90 [11] , [12] .…”

Section: Introductionmentioning

confidence: 99%

Off-label use of adipose-derived stem cells

Bertozzi

Raposio

2017

BackgroundAdipose-derived stem cells (ASCs) have a broad range of clinical applications. The ease of cell harvest and high yield with minimal donor-site morbidity makes adipose tissue an ideal source of stem cells. Further, the multi-lineage potential of these cells present significant opportunities within the field of tissue engineering, with studies successfully demonstrating their ability to produce a range of tissue types.Materials and methodsLiterature review of publications on the use of ASCs, in the context of current European and US regulations.ResultsAccording to European and US regulations, many clinical trials reported in literature to date could be considered off-label.ConclusionIn Europe, clinical trials involving cultured ASCs and/or the use of collagenase, which causes changes in the structural and functional properties of stem cells, and/or ASCs application in non-homologous tissue, should be considered off-label. ASCs should be non-cultured, isolated mechanically, and used only in the subcutaneous tissue.