Morphological changes in erythrocytes of people with type 2 diabetes mellitus evaluated with atomic force microscopy: A brief review

Loyola-Leyva, Alejandra; Loyola-Rodríguez, Juan Pablo; Atzori, Marco; González, Francisco

doi:10.1016/j.micron.2017.11.001

Cited by 17 publications

(14 citation statements)

References 30 publications

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“…Remodeling is consistently linked to risk factors such as diabetes and hypertension and involves capillary rarefaction and narrowing of capillaries and intramural arterioles. Notably, some of these risk factors is also associated with modified blood cell biophysics, such as increased RBC stiffness and adhesiveness in diabetes ( 94 ). This stiffness and adhesion change exacerbates microvascular obstruction, although much remains to be learned about blood cell biophysics in the context of CMD.…”

Section: Minding the Gap: Bridging Engineering And Microvascular Researchmentioning

confidence: 99%

Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature

Myers

Lam

2021

Annu. Rev. Biomed. Eng.

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Microengineering advances have enabled the development of perfusable, endothelialized models of the microvasculature that recapitulate the unique biological and biophysical conditions of the microcirculation in vivo. Indeed, at that size scale (<100 μm)—where blood no longer behaves as a simple continuum fluid; blood cells approximate the size of the vessels themselves; and complex interactions among blood cells, plasma molecules, and the endothelium constantly ensue—vascularized microfluidics are ideal tools to investigate these microvascular phenomena. Moreover, perfusable, endothelialized microfluidics offer unique opportunities for investigating microvascular diseases by enabling systematic dissection of both the blood and vascular components of the pathophysiology at hand. We review ( a) the state of the art in microvascular devices and ( b) the myriad of microvascular diseases and pressing challenges. The engineering community has unique opportunities to innovate with new microvascular devices and to partner with biomedical researchers to usher in a new era of understanding and discovery of microvascular diseases. Expected final online publication date for the Annual Review of Biomedical Engineering, Volume 23 is June 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Section: Minding the Gap: Bridging Engineering And Microvascular Researchmentioning

confidence: 99%

Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature

Myers

Lam

2021

Annu. Rev. Biomed. Eng.

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“…Therefore, the erythrocyte deformability is impaired by elevated membrane rigidity, increased internal viscosity or several kinds of shape changes alone or in concert [14,15]. In literature, morphological changes in diabetic erythrocytes have been reported in atomic force microscopic investigation, which include decreased biconcave depth, diameter and height [3]. Diabetic erythrocyte membrane shows phospholipid peroxidation and decreased membrane ion-transporting enzymatic activities [16].…”

Section: Discussionmentioning

confidence: 99%

“…Diabetes is associated with various hemorheologic disorders, i.e., these include platelet activation and increased whole blood viscosity and plasma viscosity. With respect to erythrocytes, morphology is changed, and deformability tends to decrease [2,3]. The deformability of erythrocytes passing through microvasculature is a prerequisite of microcirculation, and this deformability in diabetic patients has been so far investigated.…”

Section: Introductionmentioning

confidence: 99%

Impairment of Erythrocyte Deformability Observed in Type 2 Diabetic Patients with Clustering Diabetic Complications

Arita

Yamaguchi

Moriyama

et al. 2020

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Aims: Hemorheologic and microvascular dysfunction are interdependent in type 2 diabetic patients. However, exact mechanisms explaining this interaction remains unclear. This study aimed to investigate the impairment of erythrocyte deformability under concurrent recording of electrocardiogram (ECG), since heart-rate-corrected QT interval (QTc interval) prolongation reflects autonomic and microvascular dysfunction in diabetic patients. Methodology: The erythrocyte deformability was investigated on the day of digital ECG recording in diabetic (n = 215) and control (n = 88) groups of outpatients using specific filtration technique. Significant contributors to the erythrocyte deformability were analyzed by multivariate analysis. Results: Difference of mean erythrocyte deformability in the diabetic vs. control group did not reach the statistical significance, but the difference was significant in comparison of diabetic smokers vs. non-smokers and of diabetic patients with vs. without diabetic complications. Impaired diabetic erythrocyte deformability was dependent mostly on the glycated hemoglobin (HbA1c), and negative correlation between QTc interval and the deformability was marginal. Conclusions: Erythrocyte deformability was not necessarily impaired in diabetic patients under the intensive antidiabetic medication. However, this deformability was impaired in diabetic smokers and diabetic patients with clustering of complications. Future studies are required for hemorheologic and microvascular interaction leading to the impaired diabetic microcirculation.

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“…Glucose oxidation and protein glycation, caused by diabetes-associated hyperglycemia, can induce several [29] found that high glucose concentrations resulted in the glycosylation of erythrocyte membranes, thereby stiffening the glycosylated cell membranes and reducing the deformability of erythrocytes. Loyola et al [30] observed the deformability of erythrocytes via atomic force microscopy. Their results indicated that the deformability of erythrocytes in patients with DM was reduced, and their subsequent rigidity made them difficult to pass through the microvessels and led to microcirculation disturbance.…”

Section: Deformabilitymentioning

confidence: 99%

The Relationship between Erythrocytes and Diabetes Mellitus

Wang

Yang

Zhou

et al. 2021

Journal of Diabetes Research

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High blood glucose level (hyperglycemia) is a leading indicator of diabetes mellitus (DM). Erythrocytes are the most abundant cells in the circulation and the first to perceive changes in plasma composition. Long-lasting hyperglycemia affects the structure and function of erythrocytes. The detection of erythrocyte-related indicators can provide a valuable reference for the prevention, diagnosis, and treatment of DM and its complications. This paper reviews the normal structure and function of erythrocytes, the changes in erythrocytes in patients with diabetes, and the role of erythrocytes in the development of diabetic complications to provide more indicators for the early prevention of DM complications and to monitor the therapeutic effect of DM.

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Morphological changes in erythrocytes of people with type 2 diabetes mellitus evaluated with atomic force microscopy: A brief review

Cited by 17 publications

References 30 publications

Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature

Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature

Impairment of Erythrocyte Deformability Observed in Type 2 Diabetic Patients with Clustering Diabetic Complications

The Relationship between Erythrocytes and Diabetes Mellitus

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