Morphological, Biochemical, and Serological Studies on 64 Strains of
            <i>Actinomyces israelii</i>

Slack, John M.; Landfried, Sandra; Gerencser, Mary Ann

doi:10.1128/jb.97.2.873-884.1969

Cited by 38 publications

(16 citation statements)

References 19 publications

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“…However, the fact that many of these Actinomyces strains recovered from the normal pocket samples grew readily aerobically might suggest their exclusion from Actinomyces israelii. Problems with classification of oral actinomycetes have been previously described (JORDAN & HAMMOND 1972, SLOTS 1974, SPELLMAN, SOCRANSKY, HAMMOND, AM-DUR, KRICHEVSKY 1974, WILLIAMS et al 1976).…”

Section: Discussionmentioning

confidence: 91%

“…On the basis of the fermentation pattern of xylose, raffinose, and cellobiose (SLACK et al 1969) the majority of the Actinomyces isolates from deep and normal pockets could be grouped as Actinomyces israelii. However, the fact that many of these Actinomyces strains recovered from the normal pocket samples grew readily aerobically might suggest their exclusion from Actinomyces israelii.…”

Section: Discussionmentioning

confidence: 99%

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Microflora in the healthy gingival sulcus in man

Slots

1977

European J Oral Sciences

110

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– The roll tube culture technique was utilized to examine quantitatively and qualitatively the predominant cultivable microflora inhabiting the clinically healthy gingival sulcus. Seven periodontists aged 32–54 years were included in the study. From a total of 350 isolates, 85 (24.3%) were obligate anaerobes, 53 (15.0%) were Gram‐negative, and 197 (56.3 %) were categorized as rods. Actinomycetes predominated in three samples. Four samples were dominated by Streptococcus species. The flora as revealed in the present study comprised a markedly lower proportion of Gram‐negative organisms than that found at the base of deep periodontal pockets.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Microflora in the healthy gingival sulcus in man

Slots

1977

European J Oral Sciences

110

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“…Analysis of the precipitin reactions, obtained by CIE against standard antigens of A. israelii with antisera to some related bacteria, supported current classification of a separate serological group of A. israelii (5,16,(22)(23)(24). The serological reaction of the components of the cytoplasmic standard antigen of A. israelii displayed species specificity.…”

Section: Resultsmentioning

confidence: 53%

“…Antisera raised in rabbits against strains of A. israelii have in immunofluorescence, precipit in tests, and cell wall agglutination tests allowed differentiation of a distinct serogroup of' A. israelii, as well as two serotypes (4, 6,7,8,20,24).…”

mentioning

confidence: 99%

Serological studies of Actinomyces israelii by crossed immunoelectrophoresis: taxonomic and diagnostic applications

Holmberg¹,

Nord²,

Wadström³

1975

Infect Immun

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Crossed immunoelectrophoresis (CIE) with intermediate gel was applied to the serological analysis of Actinomyces israelii to develop a test with high efficiency in the laboratory diagnosis of human actinomycosis and classification of A. israelii. Recently developed standard antigen-antibody systems for A. israelii by CIE were used as reference. The reference systems were based on standard preparations of cytoplasmic and whole cell-associated antigens of A. israelii and a standard immunoglobulin G pool purified from rabbit antisera to formalintreated whole cells and cell lysates of A. israelii. The specificity of the standard antigens for A. israelii was evaluated in CIE studies by screening for antibodies to components of the antigens in rabbit antisera raised against related bacteria. The standard system for A. israelii based on cytoplasmic antigens formed speciesspecif'ic precipitins, whereas antisera raised against A. naeslundii and/or Propionibacterium acnes precipitated components of' the other standard antigens. As a result of these analyses, the standard system for A. israelii based on 10 cytoplasmic antigens was used as reference for CIE studies to detect humoral antibodies to A. israelii in sera from nine patients with actinomycosis. All the sera from the patients formed at the time of diagnosis one or more precipitins in terms of the 10 reference precipitins. Up to five precipitins were found in single sera. Follow-up studies covering a period of one-half year after treatment showed a gradually decreased precipitin response in the course of' time. In control sera from patients with newly diagnosed tuberculosis, nocardiosis, deep Candida infection, and aspergillosis, and in sera from healthy blood donors, no antibodies were detected with specificity for the reference antigens.

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“…Although their evidence was obtained through studies of the carbohydrate antigens of A. israelii, it is consistent with our data regarding the heterogeneity of type 1 strains. Other authors (6,26) have also detected such heterogeneity among type 1 strains with fluorescent-antibody techniques.…”

Section: Discussionmentioning

confidence: 91%

Identification and preliminary characterization of a major heat-stable surface antigen of Actinomyces israelii by two-dimensional (crossed) immunoelectrophoresis

Ayakawa¹,

Williams²,

Kenny³

1983

Infect Immun

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We have identified, employing two-dimensional (crossed) immunoelectrophoresis, a major heat-stable surface antigen in Actinomyces israelii with an electrophoretic mobility of 0.86 compared to bovine albumin. The surface component was protease sensitive and non-dialyzable and was found in relatively large amounts in two serotype 2 strains of A. israelii (WVU 307 and ATCC 23860). Serotype 1 strains either lacked antigen 0.86 (ATCC 12103 and CDC X522) or contained small quantities of a cross-reacting antigen which was not identical to antigen 0.86 (CDC W855). Thus, antigen 0.86 may be useful in the serotaxonomy of A. israelii. Our data also suggest the possibility that a greater degree of antigenic heterogeneity exists within the species than can be accommodated within the two currently recognized serotypes.serotypes does not accurately reflect the degree of antigenic heterogeneity within the species. 11 on August 1, 2020 by guest http://iai.asm.org/ Downloaded from

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Morphological, Biochemical, and Serological Studies on 64 Strains of Actinomyces israelii

Cited by 38 publications

References 19 publications

Microflora in the healthy gingival sulcus in man

Microflora in the healthy gingival sulcus in man

Serological studies of Actinomyces israelii by crossed immunoelectrophoresis: taxonomic and diagnostic applications

Identification and preliminary characterization of a major heat-stable surface antigen of Actinomyces israelii by two-dimensional (crossed) immunoelectrophoresis

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