Morphological aspects of intestinal cells from gilthead seabream (Sparus aurata) fed diets containing different lipid sources

Caballero, M. J.; Izquierdo, M.S.; Kjørsvik, Elin; Montero, Daniel; Socorro, J.; Fernández, A.; Rosenlund, Grethe

doi:10.1016/s0044-8486(03)00299-0

Cited by 208 publications

(168 citation statements)

References 20 publications

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“…The inclusion of VOs in carnivorous fish diets is often associated with increased fat contents and lipid droplets in liver and enterocytes, usually when substitution is over 75% (Rodríguez et al, 2002;Caballero et al, 2003;Menoyo et al, 2004;Francis et al, 2007), or at lower levels of substitution when an unbalanced fatty acid profile is provided (Caballero et al, , 2004Menoyo et al, 2004). Nevertheless, when the substitution level is not too large (50-75 %) and the fatty acid profile of the VO is well balanced, fat deposition in enterocytes and liver is not reported (Caballero et al, 2004;Mourente et al, 2005;Francis et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Gilthead seabream, Sparus aurata L., is the most important marine fish species in Mediterranean and Canarian aquaculture, and several studies have shown that it can generally be grown well on diets with FO partially replaced by VO Caballero et al, 2003Caballero et al, , 2004Menoyo et al, 2004;Benedito-Palos et al, 2007;Díaz-López et al, 2009;Fountoulaki et al, 2009). However knowledge of seabream lipid metabolism is still incomplete compared to other fish species, such as salmonids, turbot, and seabass Rodríguez et al, 2002;Mourente et al, 2005;Bell et al, 2001Bell et al, , 2002Bell et al, , 2006Tocher et al, , 2004Fonseca-Madrigal et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Effects of dietary fish oil substitution by Echium oil on enterocyte and hepatocyte lipid metabolism of gilthead seabream (Sparus aurata L.)

Díaz-López

Pérez

Acosta

et al. 2010

Comparative Biochemistry and Physiology Part B: Biochemistry an

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The fatty acid profile of vegetable oils (VOs), together with the poor ability of marine fish to convert polyunsaturated fatty acids (PUFA) to highly unsaturated fatty acids (HUFA), lead to important changes in the nutritional value of farmed fish fed VO, which include increased fat and 18:2n-6 and reduced n-3 HUFA. Echium oil (EO) has a good n-3/n-6 balance as well as an interesting profile with its high content of unusual fatty acids (SDA, was also unaffected by EO in terms of total uptake, incorporation, β-oxidation and elongationdesaturation activities.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of dietary fish oil substitution by Echium oil on enterocyte and hepatocyte lipid metabolism of gilthead seabream (Sparus aurata L.)

Díaz-López

Pérez

Acosta

et al. 2010

Comparative Biochemistry and Physiology Part B: Biochemistry an

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“…On the contrary, the lowest enterocyte lipid accumulation found in larvae fed Artemia enriched with a low dose of soybean oil is in agreement with the lowest lipid level found in this dietary treatment. It has been suggested that the dietary FA composition may alter the intestinal membrane composition, leading to changes in its morphological structure and fluidity, and may affect intestinal lipid metabolism and transport (Caballero et al, 2002(Caballero et al, , 2003. One of the suggested potential metabolic effects would be in the intracellular pathways of triacylglycerol (TAG) and phospholipid (PL) reacylation, which appear to be affected by the nature of dietary lipids Izquierdo et al, 2000;Olsen et al, 2000b).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, despite both types of Artemia being deficient in HUFA, the enrichment with soybean oil may have caused a dilution of the EFA naturally present in Artemia (mostly eicosapentaenoic acid -EPA, 20:5n-3; and arachidonic acid -ARA, 20:4n-6), possibly contributing to the observed changes in growth. In addition, lipid absorption could also have been affected by the change in Artemia FA profile, since it has been shown in carnivorous juvenile and adult fish that the inclusion of plant-derived oils may result in a reduction of the transport rate across the gut epithelia and thus in the accumulation of lipid droplets in the enterocytes, possibly leading to tissue damage and compromised gut integrity (Olsen et al, 1999(Olsen et al, , 2000b(Olsen et al, , 2003Caballero et al, 2002Caballero et al, , 2003. Besides, dietary FA profile may influence not only the composition and morphology of the intestinal cells but also the physiological mechanisms involved in intestinal lipid metabolism and transport Caballero et al, 2002Caballero et al, , 2003.…”

Section: Introductionmentioning

confidence: 99%

Dietary neutral lipid level and source in Senegalese sole (Solea senegalensis) larvae: Effect on growth, lipid metabolism and digestive capacity

Morais

Caballero

Conceição

et al. 2006

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Contrary to larval essential fatty acid (EFA) requirements, the effect of dietary neutral lipid supply has been little investigated in marine fish larvae. The present work investigates the effect of feeding Senegalese sole larvae on Artemia enriched with higher or lower doses of lipid emulsion. Two lipid sources -soybean oil and fish oil -were compared. From 16 days after hatching (DAH) onwards, larvae were fed one of four experimental treatments: Artemia enriched on a high or low dose of soybean oil emulsion (HS and LS) or Artemia enriched on a high or low dose of fish oil emulsion (HF and LF). In terms of growth, the dietary lipid level did not have a significant effect while the soybean oil treatments induced a lower growth than the fish oil-enriched Artemia. The fatty acid (FA) composition of the larvae closely reflected the dietary quantitative and qualitative FA profile. Only slight dietary effects were noted in the activity of trypsin, lipase and alkaline phosphatase. A higher amount of lipid droplets was noticeable in the posterior intestine epithelia and in the hepatocytes of larvae fed Artemia enriched with higher lipid doses, while LS-Artemia induced the lower lipid accumulation on the basal zone of the enterocytes, in accordance with the lowest total lipid level measured in this treatment. These results suggest an important effect of dietary total lipid level on lipid accumulation in the enterocytes and on FA absorption. At 33 DAH a tube feeding trial was conducted with 14 C-labelled oleic acid (OA) or triolein (TRI), showing that the lower accumulation of lipid droplets in the larvae fed LS was associated with a significantly higher absorption and retention in the gut and body tissues of the TRI label. For OA no significant differences between treatments were found. TRI label was considerably more evacuated than OA, indicating that sole larvae may have a lower capacity to incorporate a triacylglycerol, which needs to be digested. Finally, OA appears to be preferentially utilized for energy production, accumulating more in larval tissues when absorbed in higher amounts.

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“…O intestino é um órgão envolvido em importantes funções fisiológicas, sendo o principal local de digestão dos alimentos e absorção de nutrientes (Caballero et al 2003). A mucosa intestinal é fundamental nos processos digestivos, absortivo e metabólico em peixes teleósteos e o aumento do comprimento das vilosidades implica em um aumento da área de superfície para maior absorção dos nutrientes disponíveis (Caspary 1992).…”

Section: Introductionunclassified

Características histomorfométricas do intestino de juvenis de tambaqui após uso de probiótico na dieta e durante transporte

et al. 2014

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Avaliou-se neste trabalho as características histomorfométricas da mucosa intestinal de tambaqui após uso de probiótico a base de Bacillus spp., veiculado na ração e dissolvido na água durante transporte. Foi utilizado um delineamento inteiramente casualizado com três tratamentos e sete repetições, sendo comparado um tratamento controle (peixes alimentados com ração comercial sem probiótico) a dois outros tratamentos envolvendo o uso de probiótico comercial, veiculado na ração ou na água de transporte. Um total de 510 juvenis de tambaqui (peso e comprimento total médio inicial de 83,26±28,14g e 17,39±1,90cm, respectivamente) foi distribuído em três tanques de alvenaria. Durante 60 dias, os peixes de dois tanques foram alimentados com ração comercial e os do terceiro tanque com ração comercial suplementada com probiótico. Após este período, os peixes de cada tanque foram divididos em sacos plásticos e transportados por 4 horas de acordo com os seguintes tratamentos: T1 = alimentação com ração comercial (controle); T2 = alimentação com ração comercial e probiótico adicionado na água durante o transporte (20mg/L); T3 = alimentação com ração comercial suplementada com probiótico (1,0g/kg de ração). Antes do transporte (basal), 24 e 96 horas após o transporte, oito peixes de cada tratamento foram submetidos a eutanásia e o intestino retirado para pesagem e mensuração do comprimento para estabelecimento da relação comprimento corporal/ intestino. Em seguida, foi realizada coleta da porção anterior e posterior do intestino, para avaliação das características morfo-histológicas da mucosa intestinal. O uso de probiótico durante o transporte não afetou o peso do intestino de juvenis de tambaqui. O comprimento do intestino do tambaqui não foi alterado pelo tratamento, sendo observada somente relação linear entre o comprimento intestinal e o comprimento corporal (CC) dos peixes. A suplementação com probiótico (Bacillus spp.) não exerceu nenhum efeito na altura e comprimento das vilosidades do intestino do tambaqui desafiados com o transporte.

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Morphological aspects of intestinal cells from gilthead seabream (Sparus aurata) fed diets containing different lipid sources

Cited by 208 publications

References 20 publications

Effects of dietary fish oil substitution by Echium oil on enterocyte and hepatocyte lipid metabolism of gilthead seabream (Sparus aurata L.)

Effects of dietary fish oil substitution by Echium oil on enterocyte and hepatocyte lipid metabolism of gilthead seabream (Sparus aurata L.)

Dietary neutral lipid level and source in Senegalese sole (Solea senegalensis) larvae: Effect on growth, lipid metabolism and digestive capacity

Características histomorfométricas do intestino de juvenis de tambaqui após uso de probiótico na dieta e durante transporte

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