Morphological and morphometric study of Echinococcus granulosus (metacestode) in Sulaimani Province/ Kurdistan Region, Iraq

Hama, Abdullah A.; Shareef, Osama Hamid

doi:10.24017/science.2016.1.1.6

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…The results of the current study are consistent with a study (Hama & Shareef, 2016) in Sulaymaniyah city, where it found that the average total length of the LH isolated from sheep was 22.24 ± 1.300 µm, and the BL measurement of the LH which are isolated from sheep was 10.55 ± 1.700 µm. This study also recorded that the total length of the SH was 0.900 ± 17.60 µm, and the BL of the SH measured 1.00 ± 8.10 µm.…”

Section: Discussionsupporting

confidence: 92%

Morphological Characterization of Echinococcus Granulosus Isolated From Human and Sheep in Euphrates Region of Iraq

Kadhim¹,

Al-Mayali²

2021

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The study included examine of 25 samples of hydatid cysts of Echinococcus granulosus (20 human samples and 5sheep samples) During the study period from January 2019 -December 2019, Human hydatid cysts samples were collected from some hospitals in the middle Euphrates provinces, which are the hospitals of Al-Muthanna, Al-Diwaniyah, Al-Najafalashraf and Babil province hospitals, Sheep hydatid cysts samples from the animals abattoirs in the same provinces. The study aimed to determine the morphological characters of hydatid cysts and protoscoleses and differences between strains by studying the numbers of number,shape, Size and color of germinal layer of hydatid cysts also number of rostellar hooks, arrangement, measuring the total length and blade length (BL) of hooks, Size of smallhooks (SH) and large hooks(LH) for protoscoleces its shapes in studied hydatidcysts. The highest percentage of organ infection in humans was in the liver by 75%, then the lungs by 25%,no cysts the spleen and other organs was recorded. While in sheep, it was 80% in the liver and spleen by 20%, and no cysts in lung was recorded, As for the colors of the germinal layers, 60% was recorded for white color and 40% for yellowish white, and the predominant color is white in humans. And when studying the diameters cysts In the affected organs, it was found that the diameter of the cysts ranged between 5 -65 mm, No significant differences In the number and size of cysts in both hosts, the numbers of cysts ranged from 1 to more than 10 cysts. There are significant differences between the number of hooks, as it appeared that the average number of small hooks (SH) in a human is 16 hooks, While the average number of large hooks (LH) was 13.4, the total number was 29.4, While In sheep the average number of small hooks was 17.2, and the average number of large hooks (LH) was 14.6, and the total number of hooks was 32 hooks. This results may be refers to presence different of E. granolosus in human than the sheep.

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Section: Discussionsupporting

confidence: 92%

Morphological Characterization of Echinococcus Granulosus Isolated From Human and Sheep in Euphrates Region of Iraq

Kadhim¹,

Al-Mayali²

2021

PLANT ARCHIVES

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“…In general, in the case of neighbouring countries there was a low diversity in the animal hosts examined and subsequently in CE genotypes isolated, with most studies focused on E. granulosus s.s. (Utuk et al , 2008; Latif et al , 2010; Simsek et al , 2011; Eryıldız & Şakru, 2012; Hama et al , 2012; Hama & Shareef, 2016; Hasan et al , 2016; Gökpınar et al , 2017; Hassan et al , 2017). With some exceptions (Al-Qaoud et al , 2003; Trachsel et al , 2007; Ziadinov et al , 2008), the strains identified in definitive hosts in Iran are partly identical to detected genotypes in adjacent countries.…”

Section: Resultsmentioning

confidence: 99%

Echinococcus granulosus genotypes in Iran: a systematic review

et al. 2018

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Cystic echinococcosis (CE) caused by Echinococcus granulosus sensu lato (s.l.) is a significant zoonosis, especially in developing countries of the Middle East, with many studies focusing on CE genotypes in Iran. We performed a systematic review to determine the exact status of E. granulosus genotypes in the country. We explored English (Pubmed, Scopus, ISI Web of Science and Science Direct) and Persian (Magiran, Iran Medex and Scientific Information Database) databases along with Google Scholar. Our review included 73 studies published prior to the end of 2015. In total, 2952 animal (intermediate and definitive) hosts were examined, and the prevalent genotypes comprised G1 (92.75%) and G6 (4.53%) in sheep, cattle, camels, goats and buffaloes; G3 (2.43%) in five herbivore hosts and dogs; G7 (0.2%) in sheep and goats; and G2 (0.06%) in dogs. G1 was mostly dominant in West Azerbaijan, whereas G3 and G6 were identified most frequently in the provinces of Isfahan and Fars, respectively. Regarding human CE infection, 340 cases were reported from Iran, with the identified genotypes G1 (n = 320), G6 (n = 13) and G3 (n = 7). Most CE-infected humans originated from Isfahan province (168 cases), whereas the lowest number of infected persons was noted in Kerman province (two cases). The information obtained from this systematic review is central to better understanding the biological and epidemiological characteristics of E. granulosus s.l. genotypes in Iran, leading to more comprehensive control strategies.

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“…The molecular-based methods are not alternative of inspection methods for cyst detection, while the combination between direct inspection and DNA markers are important to understanding the nature and origin of cysts [31] The morphological and biochemical markers are helpful for species and strain identification of Taenia spp. while the molecular and DNA markers are more accurate and dependable for molecular epidemiology study [32]. The DNA marker and molecular characterization are valuable for species and strain identification of Taenia species [33,34].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Molecular Characterization of Cysticercus tenuicollis Isolated from Some Intermediate Host in Kurdistan-Iraq

Hama

Zorab²,

Ali

et al. 2018

KJAR

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Cysticercus tenuicollis (C. tenuicollis) is the larval stage infection of Taenia hydatigena, a common tapeworm of dogs and other Canidae, which has a wide range of intermediate hosts including sheep, goat, cattle, deer, camel, horse, human and other wild ruminants, the disease spreads through a contaminated water, soil and food with feces of infected dogs or other carnivores, T. hydatigena lives in the intestinal of the definitive host (carnivores) and excretes a huge number of eggs with feces daily. The present study conducted to determine the prevalence rate and molecular characterization of C. tenuicollis among sheep and goats in Sulaimani province. A total of 14088 slaughtered animals were inspected postmortem from the new Sulaimani abattoir comprise, which involves 13395 sheep and 693 goats. The selected cysts were preserved in 70% Ethanol for DNA extraction and molecular study, The mt-CO1 gene was amplified with a conventional polymerase chain reaction (PCR), the PCR product purified and DNA sequencing for reverse and forward strands was determined by a genetic analyzer, the obtained sequences aligned with the DNA sequences of T. hydatigena in Iran, Turkey and Palestine, which deposited in GenBank under the following accession number (JQ710588), (JN827307) and ( KM032284) respectively. The prevalence rate of C. tenuicollis was 2.63% in sheep and 2.58% in goats. This result shows no significant differences of C. tenuicollis between sheep and goats (p>0.05). The nucleotide sequence alignment of cytochrome oxidase subunit 1 (CO 1) gene revealed that the amplified DNA fragment is belong to Taenia hydatigena and Echinococcus granulosus and the nucleotide sequences of T. hydatigena deposited in GenBank under accession number (MH638348). This finding concludes that the amplification of mt-CO1 gene cannot be depended on discriminate hydatid cyst and C. tenuicollis while the partial DNA sequences of mt-CO1 gene are significantly valuable to differentiate C. tenuicollis from hydatid cyst, which is completely different in the pathology and control.

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Morphological and morphometric study of Echinococcus granulosus (metacestode) in Sulaimani Province/ Kurdistan Region, Iraq

Cited by 4 publications

References 13 publications

Morphological Characterization of Echinococcus Granulosus Isolated From Human and Sheep in Euphrates Region of Iraq

Morphological Characterization of Echinococcus Granulosus Isolated From Human and Sheep in Euphrates Region of Iraq

Echinococcus granulosus genotypes in Iran: a systematic review

Prevalence and Molecular Characterization of Cysticercus tenuicollis Isolated from Some Intermediate Host in Kurdistan-Iraq

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