Morphological and molecular studies of <i>Sphaerospermopsis torques-reginae</i> (Cyanobacteria, Nostocales) from South American water blooms

Werner, Vera Regina; Laughinghouse, Haywood Dail; Fiore, Marli Fátima; Sant’Anna, Célia Leite; Hoff, Caroline; Santos, Kleber Renan de Souza; Neuhaus, Emanuel Bruno; Molica, Renato José Reis; Honda, Ricardo Yukio; Echenique, Ricardo Omar

doi:10.2216/11-32.1

Cited by 41 publications

(26 citation statements)

References 25 publications

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“…So far, only a few findings of S. reniformis or other coiled species with a similar morphology (S. torques reginae, A. eucompacta, A. oumiana) have been reported from water bodies in Africa, Asia, Europe and Central and South America (Li and Watanabe 1999;Cronberg and Annadotter 2006;Zapomělová et al 2009;Werner et al 2012). However, this dearth of reports could be attributed to misidentification of this morphospecies (Cronberg and Annadotter 2006;Werner et al 2012).…”

Section: R Curvata From Hartbeespoort Dam Than To Cylindrospermopsismentioning

confidence: 99%

Diversity of cyanobacteria and cyanotoxins in Hartbeespoort Dam, South Africa

Ballot

Sandvik

Rundberget

et al. 2014

Mar. Freshwater Res.

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Abstract. The South African Hartbeespoort Dam is known for the occurrence of heavy Microcystis blooms. Although a few other cyanobacterial genera have been described, no detailed study on those cyanobacteria and their potential toxin production has been conducted. The diversity of cyanobacterial species and toxins is most probably underestimated. To ascertain the cyanobacterial composition and presence of cyanobacterial toxins in Hartbeespoort Dam, water samples were collected in April 2011. In a polyphasic approach, 27 isolated cyanobacterial strains were classified morphologically and phylogenetically and tested for microcystins (MCs), cylindrospermopsin (CYN), saxitoxins (STXs) and anatoxin-a (ATX) by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and screened for toxin-encoding gene fragments. The isolated strains were identified as Sphaerospermopsis reniformis, Sphaerospermopsis aphanizomenoides, Cylindrospermopsis curvispora, Raphidiopsis curvata, Raphidiopsis mediterrranea and Microcystis aeruginosa. Only one of the Microcystis strains (AB2011/53) produced microcystins (35 variants). Forty-one microcystin variants were detected in the environmental sample from Hartbeespoort Dam, suggesting the existence of other microcystin producing strains in Hartbeespoort Dam. All investigated strains tested negative for CYN, STXs and ATX and their encoding genes. The mcyE gene of the microcystin gene cluster was found in the microcystin-producing Microcystis strain AB2011/53 and in eight non-microcystin-producing Microcystis strains, indicating that mcyE is not a good surrogate for microcystin production in environmental samples.

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Section: R Curvata From Hartbeespoort Dam Than To Cylindrospermopsismentioning

confidence: 99%

Diversity of cyanobacteria and cyanotoxins in Hartbeespoort Dam, South Africa

Ballot

Sandvik

Rundberget

et al. 2014

Mar. Freshwater Res.

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“…9, the two strains were grouped into a cluster with a high bootstrap support, together with all other Sphaerospermopsis species already verified, including the coiled species S. torques-reginae, S. reniformis and S. oumiana (Zapomeˇlovaé t al. 2009;Tuji & Niiyama 2010;Werner et al 2012) and the straight species S. kisseleviana (Elenkin) Zapomeˇlova´et al and S. aphanizomenoides (Zapomeˇlova´et al 2009). The phylogenetic tree based on the 16S-23S ITS gene showed the same result.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and genotypic validation of the rare species Sphaerospermopsis eucompacta comb. nov. (Nostocales, Cyanobacteria) isolated from China

Yang

2015

Phycologia

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The heterocystous cyanobacterium Anabaena eucompacta R. Li & M. Watanabe was found in China, and two unialgal strains were isolated. The species was characterized by its twisted and tight spirals and the relative position of spherical akinetes to heterocysts (adjacent to one or both sides of heterocysts). However, the taxonomic and phylogenetic position of the species remained to be characterized because of the lack of molecular data. In this study, we used a polyphasic approach, based on both morphological and molecular characters (sequences of 16S rRNA and 16S-23S rRNA), to evaluate its taxonomic position. Our results showed that A. eucompacta was a distinct species and that it should be transferred to the recently established genus Sphaerospermopsis as S. eucompacta (R. Li & M. Watanabe) X. Li & R. Li comb. nov.

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“…Recent research of eutrophicated lakes suggests, however, increasing occurrence of filamentous cyanobacteria such as Planthothrix, Aphanizomenon, Dolichospermum (Anabaena) and Cylindrospermopsis (Krupa & Czernaś 2003, Stefaniak & Kokociński 2005, Błaszczyk et al 2010, Fuentes et al 2010, Ferrari et al 2011, Kokociński et al 2011, Werner et al 2012. As a consequence, there are growing issues with the use of drinking water reservoirs and also with the decrease in quality of water in recreational basins, both natural and artificial water ecosystems (Codd 2000, Molica et al 2005, Haande et al 2007, Bláha et al 2009, Duan et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, some cyanobacteria produce toxic substances that are very dangerous for oxygen sensitive organisms (Jura & Krzanowska 1999, Romanowska-Duda et al 2002, Mazur et al 2003, Babica et al 2006, Werner et al 2012. These substances can be deadly to water animals (anthropoda, mollusca, fish), land animals (birds, cattle) (Bucka 1998, Bucka & Wilk-Woźniak 1999, Oudra et al 2002, Pawlik-Skowrońska et al 2004, Mankiewicz et al 2005) and even humans (Barreto & Figueiredo 1996, Kuiper-Goodman et al 1999, Carmichael 2001, Oudra et al 2002, Bláha et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Anabaena poulseniana J. Boye Petersen — a species new to Polish flora

Richter

2013

Oceanological and Hydrobiological Studies

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Anabaena poulseniana J. Boye Petersen Bot. Iceland 2, 1923 (Cyanobacteria), a cyanobacteria species new to Polish flora, was noted during a study of phytoplankton in one of Wrocław's clay pits. The species was noted in plankton samples among Cylindrospermopsis raciborskii (Wołoszyńska) Seenayya et Subba Raju (Cyanobacteria) and Mougeotia sp. (Chlorophyta) filaments. The green algae created a bloom characterized by vegetation mats floating on the water.The paper presents detailed characteristics for Anabaena poulseniana, and for the habitat and the ecological condition in the examined water reservoir during its presence. The paper also presents a comparison of morphological characteristics of A. poulseniana from Poland to the species from other geographical locations. The research will provide data on the morphology and ecology of the species. The species found during the study were documented in the form of original photos and illustrations. *

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Morphological and molecular studies of Sphaerospermopsis torques-reginae (Cyanobacteria, Nostocales) from South American water blooms

Cited by 41 publications

References 25 publications

Diversity of cyanobacteria and cyanotoxins in Hartbeespoort Dam, South Africa

Diversity of cyanobacteria and cyanotoxins in Hartbeespoort Dam, South Africa

Phenotypic and genotypic validation of the rare species Sphaerospermopsis eucompacta comb. nov. (Nostocales, Cyanobacteria) isolated from China

Anabaena poulseniana J. Boye Petersen — a species new to Polish flora

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