Morphological and molecular confirmation of the occurrence of Pyropia tanegashimensis (Bangiales, Rhodophyta) from Palaui Is., Sta. Ana, Cagayan, Philippines

Abstract

The known morphological features in identifying common species of foliose Bangiales (e.g., members of Pyropia and Porphyra), which are very few and often overlapping, are recently resolved using comparative analysis of DNA sequences and statistics. Records of foliose Bangiales in the Philippines were historically identified based on morphology. Considering the recent radical changes in the taxonomy among these rhodophytes and problems posed by morphology-based identification, taxonomic re-appraisal of Philippine foliose Bangiales based on critical morphological and molecular studies is needed. This study used plastid rbcL and mitochondrial COI-5P gene sequences to investigate the identity of foliose Bangiales collected in Palaui Is., Sta. Ana, Cagayan, Philippines. Observation of key phenotypes revealed the identity of the collected materials as Py. tanegashimensis. Resulting phylogenetic trees showed placement of our collected specimens into a highly supported Py. tanegashimensis clade from Japan and Brazil. Our molecular analysis also suggested that the Philippine Py. tanegashimensis includes endemic populations distinct from the introduced strain originally reported from Brazil.

Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile

“…morphology in this group of algae would have been shaped by the particular genetic, developmental, and environmental constraints operating on these algal taxa (Rosen 1967). The rapid and accurate identification of local genetic diversity in foliose Bangiales, recently achieved using molecular techniques (Schweikert et al 2012, Nelson 2013, Dumilag et al 2016, Guillemin et al 2016, Reddy et al 2018, Yang et al 2018, will allow researchers to refine the knowledge about the intrinsic and environmental determinants of their distribution across tidal gradients of contrasting habitats and climates.…”

“…Of particular note, most of the "nori" species cultivated in Asia now belong in Pyropia (Sutherland et al 2011, Zuccarello 2011. The delimitation of species was undertaken recently using molecular tools, an advent that has redefined the boundaries of former taxa over the last 10 years (e.g., Broom et al 2002, Brodie et al 2007, Neefus et al 2008, Nelson 2013, Dumilag et al 2016, Guillemin et al 2016, Reddy et al 2018, Yang et al 2018. A high level of cryptic genetic diversity was found within Porphyra and Pyropia (Brodie et al 2007, Niwa et al 2009, Lindstrom et al 2015, Guillemin et al 2016, Koh and Kim 2018, Reddy et al 2018, Yang et al 2018.…”

Genomic diversity of 39 samples of Pyropia species grown in Japan

“…Prior to the genomic era, various studies analyzed the genetic diversity of Pyropia species. For example, studies used the methods based on simple sequence repeat [8,9], DNA sequencing of several genes [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28], amplified fragment length polymorphism [29,30], and polymerase chain reaction-restriction fragment length polymorphism [31]. These previous studies analyzed small regions of the genome.…”