“…Although they have a long evolutionary history, considerable difficulty has arisen when studying phylogenetic relationships using morphological features due to the simplicity, convergent evolution, and speciation by polyploidization associated with these plants (Hickey et al, 1989;Hoot et al, 2004;Kim et al, 2010b). In addition, the Isoёtes taxa are highly endemic due to limited local distributions and obtaining samples can be difficult because they are endangered plants with small populations Jung et al, 2009Jung et al, , 2013. Therefore, studies are underway to delimit Isoёtes species taxonomically using various molecular markers or to analyze the phylogenetic relationships among the species Hoot et al, 2006;Kim et al, 2009aKim et al, , 2010b.…”