Morphological and molecular characterization of actinic lentigos reveals alterations of the dermal extracellular matrix

Warrick, Emilie; Duval, Christine; Nouveau, Stéphanie; Bastien, Philippe; Piffaut, Virginie; Chalmond, Bernard; Jp, Ortonne; Lacharriére, Olivier de; Bernerd, Françoise

doi:10.1111/bjd.15697

Cited by 13 publications

(28 citation statements)

References 56 publications

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“…It is frequently found on photoexposed areas such as the hand, face, and upper back. Histological observations confirmed that melanin accumulates in the actinic lentigo epidermis mostly in the basal layer [ 309 , 310 , 311 ]. Such lentigo is also characterized by deep epidermal invaginations that form club-shaped or bud-like extensions into the dermis.…”

Section: Mid- and Long-term Consequences Of Uv Exposurementioning

confidence: 75%

“…Such lentigo is also characterized by deep epidermal invaginations that form club-shaped or bud-like extensions into the dermis. Melanocyte activation is controversial, as their density along the dermal epidermal junction has been found to be similar in lesional compared to perilesional skin [ 310 , 311 ]. Of interest, transcriptomic studies revealed multiple molecular alterations notably dysregulation of keratinocyte proliferation and differentiation and modifications of the dermal extracellular matrix suggesting that keratinocytes and the dermal compartment could play a crucial role in the physiopathology of lentigines [ 310 , 311 , 312 ].…”

Section: Mid- and Long-term Consequences Of Uv Exposurementioning

confidence: 99%

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Clinical and Biological Characterization of Skin Pigmentation Diversity and Its Consequences on UV Impact

Bino

Duval

Bernerd

2018

IJMS

184

138

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Skin color diversity is the most variable and noticeable phenotypic trait in humans resulting from constitutive pigmentation variability. This paper will review the characterization of skin pigmentation diversity with a focus on the most recent data on the genetic basis of skin pigmentation, and the various methodologies for skin color assessment. Then, melanocyte activity and amount, type and distribution of melanins, which are the main drivers for skin pigmentation, are described. Paracrine regulators of melanocyte microenvironment are also discussed. Skin response to sun exposure is also highly dependent on color diversity. Thus, sensitivity to solar wavelengths is examined in terms of acute effects such as sunburn/erythema or induced-pigmentation but also long-term consequences such as skin cancers, photoageing and pigmentary disorders. More pronounced sun-sensitivity in lighter or darker skin types depending on the detrimental effects and involved wavelengths is reviewed.

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Section: Mid- and Long-term Consequences Of Uv Exposurementioning

confidence: 75%

Section: Mid- and Long-term Consequences Of Uv Exposurementioning

confidence: 99%

Clinical and Biological Characterization of Skin Pigmentation Diversity and Its Consequences on UV Impact

Bino

Duval

Bernerd

2018

IJMS

184

138

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“…Different authors could not find pigmentation genes differentially expressed in SL. In stead, genes which are involved in the keratinization process, such as SPRR4 [20] or LCE1E were detected, and sciellin was proved to be downregulated [20, 21]. Cario-Andre et al [22] proposed retaining perilesional pigment-harboring keratinocytes as a causative factor.…”

Section: Discussionmentioning

confidence: 99%

Keratinocytic Malfunction as a Trigger for the Development of Solar Lentigines

et al. 2019

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Introduction: Solar lentigines (SL) affect chronically UV-radiated skin. Treatment is often refractory. Deeper knowledge on its pathogenesis might improve therapeutic effects. Material and Methods: Morphological characterization of 190 SL was performed and epidermal thickness, pigment distribution, dendricity, and cornification grade were measured. Immunoreactivity was investigated using Melan A, Tyrosinase, MITF, p53, and CD20, as well as Notch1 using immunofluorescence. Results: We found 2 groups of histological patterns, i.e., either acanthotic or atrophic epidermis. Lesions with basket-woven cornification and atrophic epidermis were observed in 6 out of 9 and 14 out of 16 cases from the face, respectively. Consistency of areas with a high pigmentation was observed in 96–97% of the cases. Hyperpigmentation grade and acanthosis or cornification disorders correlated positively in 88.5% of the cases. Overexpressed of p53 was found in 19 out of 20 lesions, presenting in a scattered distribution. A significant correlation of p53 and acanthosis (p = 0.003) and cornification grade (p = 0.0008) was observed. Notch1 was expressed in all SL, with the highest immunoreactivity in atrophic facial lesions. Lesions from the hands expressed Notch1 mainly in acanthotic areas with elongated rete ridges and less compact cornification. Discussion: We suggest that Notch1-dependent keratinocytic malfunction causes the development of SL. Consequently, hyperpigmentation would be a result and not the primary cause of the pathogenesis. Confirmation of these findings might have clinical implications as hitherto treatment has mainly focused on melanocytes and pigmentation and not on the proliferation/differentiation balance of keratinocytes.

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“…Next, we focused on the genes highly expressed in each region (Supplementary Fig. 1c) (23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38). Genes encoding ELOVL fatty acid elongase and 5 (ELOVL3 and ELOVL5), perilipin 2 and (PLIN2 and PLIN5), and microsomal glutathione S-transferase 1 (MGST1) are highly expressed in sebaceous glands (23)(24)(25)(26).…”

Section: The Ssl-rna Expression Profile Predominantly Reflects Mrna Expression In Sebaceous Glands Epidermis and Hair Folliclesmentioning

confidence: 99%

Non-invasive human skin transcriptome analysis using mRNA in skin surface lipids

Inoue

Kuwano

Uehara

et al. 2021

Preprint

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Non-invasive acquisition of mRNA data from the skin would be extremely useful for understanding skin physiology and diseases. Inspired by the holocrine process, in which the sebaceous glands secrete cell contents into the sebum, we focused on the possible presence of mRNAs in skin surface lipids (SSLs). We found that measurable human mRNAs exist in SSLs, where sebum protects them from degradation by RNases. The AmpliSeq transcriptome analysis was modified to measure SSL-RNAs, and our results revealed that SSL-RNAs predominantly contained mRNAs derived from sebaceous glands, epidermis, and hair follicles. Analysis of SSL-RNAs non-invasively collected from patients with atopic dermatitis revealed significantly increased expression of inflammation-related genes and decreased expression of terminal differentiation-related genes, consistent with the results of previous reports. Further, we found that lipid synthesis-related genes were downregulated in the sebaceous glands of patients with atopic dermatitis. These results indicate that the analysis of SSL-RNAs is promising to understand the pathophysiology of skin diseases.

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Morphological and molecular characterization of actinic lentigos reveals alterations of the dermal extracellular matrix

Cited by 13 publications

References 56 publications

Clinical and Biological Characterization of Skin Pigmentation Diversity and Its Consequences on UV Impact

Clinical and Biological Characterization of Skin Pigmentation Diversity and Its Consequences on UV Impact

Keratinocytic Malfunction as a Trigger for the Development of Solar Lentigines

Non-invasive human skin transcriptome analysis using mRNA in skin surface lipids

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