Morphological and Molecular Characterization of Polycentric Rumen Fungi Belonging to the Genus Orpinomyces Isolated from Indian Cattle and Buffaloes

Sridhar, Manpal; Kumar, Deepak; Anandan, Shalini; Prasad, Chandra Shekhar; Kt, Sampath

doi:10.3923/jm.2010.581.594

Cited by 9 publications

(4 citation statements)

References 40 publications

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Section: Proposed Criteria For Neocallimastigomycota Characterizationmentioning

confidence: 99%

“… ||Continuous subculturing and ‘domestication’ of isolates could lead to changes in an isolate’s microscopic features. For example, polycentric taxa often cease to produce sporangia or zoospores with continuous subculturing [46], and hence microscopic characterization of such taxa should be undertaken promptly post isolation. ¶Recommended primers to use are ITS1F-NL4R [24, 47]. #Cloning and sequencing the entire operon and bioinformatically extracting ITS1 and LSU region for phylogenetic analysis could be a substitute for individual ITS1 and LSU amplification and sequencing described above.…”

Section: Proposed Criteria For Neocallimastigomycota Characterizationmentioning

confidence: 99%

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Characterization and rank assignment criteria for the anaerobic fungi (Neocallimastigomycota)

Elshahed

Hanafy

Cheng

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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Establishing a solid taxonomic framework is crucial for enabling discovery and documentation efforts. This ensures effective communication between scientists as well as reproducibility of results between laboratories, and facilitates the exchange and preservation of biological material. Such framework can only be achieved by establishing clear criteria for taxa characterization and rank assignment. Within the anaerobic fungi (phylum Neocallimastigomycota), the need for such criteria is especially vital. Difficulties associated with their isolation, maintenance and long-term storage often result in limited availability and loss of previously described taxa. To this end, we provide here a list of morphological, microscopic, phylogenetic and phenotypic criteria for assessment and documentation when characterizing newly obtained Neocallimastigomycota isolates. We also recommend a polyphasic rank-assignment scheme for novel genus-, species- and strain-level designations for newly obtained Neocallimastigomycota isolates.

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Section: Proposed Criteria For Neocallimastigomycota Characterizationmentioning

confidence: 99%

Section: Proposed Criteria For Neocallimastigomycota Characterizationmentioning

confidence: 99%

Characterization and rank assignment criteria for the anaerobic fungi (Neocallimastigomycota)

Elshahed

Hanafy

Cheng

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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“…The genomic DNA of the probiotic lactic acid antibiotics from the feed of farm animals has bacterial strains was extracted using Cetyl Trimethyl increased the interest of using alternative natural Ammonium bromide (CTAB) method as described by products that allow maintenance of high Chen et al (2006) and Sridhar et al (2010) with little productivity and reduction of morbidity and modifications. mortality in intensive farms.…”

Section: Introductionmentioning

confidence: 99%

Detection and molecular characterization of butyrate-producing genes in probiotic lactic acid bacteria for use in livestock

Oloyede¹,

Albert²,

Arowosegbe³

2017

Nig. J. Biotechnol

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“…Prevention or control of enteric diseases in animals has been reported to be achieved by the Molecular characterization of probiotic lactic acid incorporation of antibiotics in the feed of the bacteria by 16S rRNA gene sequencing animals. However, the demand for withdrawal of The genomic DNA of the probiotic lactic acid antibiotics from the feed of farm animals has bacterial strains was extracted using Cetyl Trimethyl increased the interest of using alternative natural Ammonium bromide (CTAB) method as described by products that allow maintenance of high Chen et al (2006) and Sridhar et al (2010) with little productivity and reduction of morbidity and modifications. mortality in intensive farms.…”

Section: Introductionmentioning

confidence: 99%

Detection and molecular characterization of butyrate-producing genes in probiotic lactic acid bacteria for use in livestock

Oloyede¹,

Albert²,

Arowosegbe³

2017

Nig. J. Biotechnol

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Butyrate-producing gut microflora synthesizes and secretes butyrate which serves as source of energy and stimulates rumen development in young animals. The present study was undertaken to characterize and screen butyrate-producing lactic acid bacteria (LAB) for probiotic use in animals in order to manipulate their gut flora for the benefit of host health and productivity. Twenty strains of LAB isolates from faeces of West African Dwarf (WAD) goats were screened for probiotic potentials. The potential probiotic LAB were characterized by 16S rRNA gene sequencing method. Polymerase chain reaction was then used to detect the butyrate kinase (buk) gene in probiotic LAB strains. The 16S rRNA gene sequencing identified the potential probiotic LAB as strains of Lactobacillus acidophilus, L. reuteri, L. plantarum, L. helveticus and L. fermentum. Their probiotic potentials were demonstrated by their ability to tolerate low pH, bile acid and lysozyme. The PCR analysis revealed that gene encoding butyrate kinase is present in only Lactobacillus plantarum PLB5. The study revealed that these LAB strains could be developed into useful probiotics in improving the health, nutrient digestibility and growth performance of livestock, but only L. plantarum PLB5 possesses the ability to produce butyrate.

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Morphological and Molecular Characterization of Polycentric Rumen Fungi Belonging to the Genus Orpinomyces Isolated from Indian Cattle and Buffaloes

Cited by 9 publications

References 40 publications

Characterization and rank assignment criteria for the anaerobic fungi (Neocallimastigomycota)

Characterization and rank assignment criteria for the anaerobic fungi (Neocallimastigomycota)

Detection and molecular characterization of butyrate-producing genes in probiotic lactic acid bacteria for use in livestock

Detection and molecular characterization of butyrate-producing genes in probiotic lactic acid bacteria for use in livestock

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