Morphological and molecular characterization of Cladosporium cladosporioides species complex causing pecan tree leaf spot

Walker, Colin; Muniz, Marlove Fátima Brião; Rolim, Jessica Mengue; Martins, Rogélia; Rosenthal, Victória Cozer; Maciel, Caciara Gonzatto; Mezzomo, Ricardo; Reiniger, Lia Rejane Silveira

doi:10.4238/gmr.15038714

Cited by 25 publications

(14 citation statements)

References 17 publications

(22 reference statements)

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“…They also reported that the conidia of C. cladosporioides are differing in shape from ellipsoid to thin-walled limoniform, and forming long, branched, pale olive-brown chains, most without a septum, with similar coloring to conidia, and one or no septa. On its own, the morphological key used to identify species of the C. cladosporioides complex [1] did not allow differentiation of the present isolates, thus it was necessary to compare this data with molecular phylogeny [32]. Our results are consistent with the possible presence of cryptic species of C. cladosporioides lineages [1].…”

Section: Resultssupporting

confidence: 71%

“…These conidia are numerous, in chains of up to nine conidia and also they are limoniform, ovoid, obovoid, aseptate, light brown, hila conspicuous. The colonies of C. Cladosporioides were greenish grey to dark greenish grey, whereas the reverse of the plate revealed a greenish-black coloration [32]. They also reported that the conidia of C. cladosporioides are differing in shape from ellipsoid to thin-walled limoniform, and forming long, branched, pale olive-brown chains, most without a septum, with similar coloring to conidia, and one or no septa.…”

Section: Resultsmentioning

confidence: 95%

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Identification and application of a fungal biocontrol agent Cladosporium cladosporioides against Bemisia tabaci

Islam

Gupta

Surovy

et al. 2019

Biotechnology & Biotechnological Equipment

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The entomopathogenic fungus Cladosporium cladosporioides is a potential candidate for biocontrol of insect pests. We isolated a strain of C. cladosporioides BOU1 from an infected brown plant hoper (BPH) of rice and characterized it using morpho-physiological and molecular analyses. Internal transcribed spacer regions and intervening 5.8S rRNA gene (ITS) sequencing and morphopathogenic analyses confirmed that BOU1 is a strain of C. cladosporioides. To select the suitable medium for this fungus, a single condium of BOU1 was grown in potato dextrose agar (PDA), potato dextrose agar with yeast (PDAY), Sabouraud dextrose agar (SDA) and synthetic nutrientpoor agar (SNA) media. The suitable medium for this fungal isolate was determined by fungal growth (colony area and conidiogenesis), and enzymatic activities (protease and lipase). The fungal growth parameters including enzymatic activities showed that the PDA medium is most suitable culture medium for C. cladosporioides. Finally, the pathogenicity of this fungal isolate was evaluated against whitefly, Bemisia tabaci through direct contact toxicity assay on eggplant leaves by dipping under laboratory conditions. The BOU1 strain caused mortality in B. tabaci in a dosedependent manner, the highest mortality being 71% at 1 Â 10 8 conidia/mL. To the best of our knowledge, this is the first report of isolation and molecular characterization of an entomopathogenic fungus C. cladosporioides from a BPH of rice. This study suggests that BOU1 is a potential candidate for biological control of whitefly for the promotion of sustainable agriculture.

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Section: Resultssupporting

confidence: 71%

Section: Resultsmentioning

confidence: 95%

Identification and application of a fungal biocontrol agent Cladosporium cladosporioides against Bemisia tabaci

Islam

Gupta

Surovy

et al. 2019

Biotechnology & Biotechnological Equipment

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“…isolates. According to Walker et al (2016), the multivariate analysis UPGMA was efficient in the separation of 40 isolates of Cladosporium spp. obtained from pecan nut into 5 groups with 30% of dissimilarity.…”

Section: Discussionmentioning

confidence: 99%

Morphological and Molecular Characterization of Fusarium Spp. Pathogenic to Ilex Paraguariensis

Mezzomo

Rolim

Poletto

et al. 2018

CERNE

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MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF Fusarium spp. PATHOGENIC TO Ilex Paraguariensis MEZZOMO, R.; ROLIM, J. M.; POLETTO, T.; ROSENTHAL, V. C.; SAVIAN, L. G.; REINIGER, L. R. S.; MUNIZ , M. F. B.Morphological and molecular characterization of Fusarium spp. pathogenic to Ilex Paraguariensis. CERNE, v. 24, n. 3, p. 209-218, 2018. HIGHLIGHTSDue to the low initial costs and ease of employment of agrosilvopastoral systems, the cultivation of yerba-mate constitutes an alternative for small properties in the RS to the diversification of production.The isolates of Fusarium spp. were submitted to the UPGMA analysis and grouped by the similarity between the characters averages.Through the UPGMA clustering test, the thirty-nine isolates of Fusarium spp. were allocated to seven large groups.The analysis of the TEF 1-α gene region was enough to discriminate species of Fusarium spp.The reflex symptoms resulted from the interaction of Fusarium spp. isolates with yerbamate roots. ABSTRACTThe cultivation of yerba-mate (Ilex paraguariensis) has been intensified in the State of Rio Grande do Sul, as result of the valuation of the price paid to the producer. However, studies related to Fusarium pathosystem vs. yerba-mate are still insufficient, so that many producers have faced significant losses in herbal production due to the occurrence of the roots rotting. Considering the above, the objectives of this study were: a) morphologically characterize the Fusarium spp. isolates collected in sick plants of yerba-mate; b) select efficient morphological characters on separating the Fusarium spp. isolates in similarity groups; c) identify the Fusarium spp. isolates to species level through the sequencing of the genomic region of elongation factor 1-α (TEF-1α); and d) testing the pathogenicity of the Fusarium spp. isolates selected based on the morphological characterization. For such, collections for the pathogen isolates were made in five cities and the collection points were georeferenced. Thirty-nine isolates were morphologically characterized by variables as mycelial growth, sporulation, macroconidia length and width, microconid format, colonies pigmentation and formation of chlamydospore, and tested for pathogenicity by substrate inoculation. The variables used in the morphological characterization were efficient in discriminating the isolates in seven groups, especially length of macronidia associated to sporulation. Seven pathogenic isolates of Fusarium spp. were identified, also through molecular sequencing of the TEF-1α gene region. The sequencing of the TEF-1α region consolidated the identification of pathogenic isolates as F. solani and F. oxysporum. v.24 n.3 2018 MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF FUSARIUM SPP. PATHOGENIC TO Ilex Paraguariensis 210 CERNE MEZZOMO et al.

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“…Isolates used were pathogenic to pecan trees, which were obtained from leaves that presented leaf spot symptoms collected from orchards located in municipalities of the three states of southern Brazil (Table 1) (Walker et al, 2016b).…”

Section: Methodsmentioning

confidence: 99%

Antagonism and Effect of Volatile Metabolites of Trichoderma spp. on Cladosporium spp.

et al. 2019

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Pecan is susceptible to the occurrence of diseases capable of harming its development, and leaf spot caused by Cladosporium spp is among them. Therefore, the objective of this work was to analyze the potential antagonist and in vitro effect of volatile metabolites of Trichodermaspp. On Cladosporium spp. isolated from pecan leaf spots. Three Cladosporium spp. isolates and two commercial products based on Trichodermaspp. were used, as well as an isolate of the same genus provided by the 'ElocyMinussi' Laboratory of Phytopathology at UFSM. Cladosporium spp. mycelial growth was monitored in order to evaluate the morphological characteristics of the pathogen. The use of Trichoderma spp. as biocontrol was assessed through dual culture tests and production of volatile metabolites. Trichoderma spp. showed positive results in inhibiting the growth of Cladosporium spp., thereby presenting potential to be used as a biocontrol agent.

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Morphological and molecular characterization of Cladosporium cladosporioides species complex causing pecan tree leaf spot

Cited by 25 publications

References 17 publications

Identification and application of a fungal biocontrol agent Cladosporium cladosporioides against Bemisia tabaci

Identification and application of a fungal biocontrol agent Cladosporium cladosporioides against Bemisia tabaci

Morphological and Molecular Characterization of Fusarium Spp. Pathogenic to Ilex Paraguariensis

Antagonism and Effect of Volatile Metabolites of Trichoderma spp. on Cladosporium spp.

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