Morphological and molecular characterisation of Hemicycliophora lutosa Loof & Heyns, 1969 and H. typica de Man, 1921 from South Africa (Nematoda: Hemicycliophoridae)

Abstract

Two Hemicycliophora species, H. lutosa and H. typica, found in samples from fallow soil and sugarcane soil in South Africa, were studied morphologically and, for the first time, molecularly. Diagnostic PCR-IT-rRNA-RFLP profiles generated by five restriction enzymes are provided. Study of phylogenetic relationships using D2-D3 expansion segment of 28S rRNA gene sequences revealed that H. lutosa was related to H. poranga. Hemicycliophora lutosa and H. poranga are compared morphologically. SEM photographs are given for H. typica and for H. lutosa for the first time. The male of H. typica represents a first report for South Africa.

Morphological and molecular characterisation of two new Hemicycliophora species (Tylenchida: Hemicycliophoridae) with a revision of the taxonomic status of some known species and a phylogeny of the genus

“…The newly obtained sequences of the D2-D3 of 28S rRNA gene, the ITS rRNA gene and the COI gene were aligned using ClustalX 1.83 (Thompson et al, 1997) with default parameters, with corresponding pub- botin et al, 2005Van den Berg et al, 2010;Cordero López et al, 2013;Powers et al, 2014;Ma & Agudelo, 2015;Zeng et al, 2015). Outgroup taxa for each dataset were chosen as shown in the previous study (Subbotin et al, 2014).…”

“…Since then it has been found worldwide associated with numerous plants and habitats . From South Africa it has been described several times with detailed descriptions and morphometrics (Heyns, 1962;Loof & Heyns, 1969;Van den Berg, 1981;Van den Berg et al, 2010). Subsequent reports of the South African populations only included morphometrics and SEM micrographs.…”