Morphological and histochemical ageing changes in patellar articular cartilage of the rat

Schiefke, Ingolf; Weiss, J.; Keller, F.; Leutert, G

doi:10.1016/s0940-9602(98)80054-x

Cited by 6 publications

(10 citation statements)

References 16 publications

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“…The presence of LDH in joint cartilage has been documented in various species including the dog (Altman 1981;Dunham et al 1986;Pelletier et al 1985;Schiefke et al 1998;Tushan et al 1969;Weseloh and Fiesselmann 1975). In tibial plateau cartilage of normal dogs, LDH was reported to be homogeneously spread in superWcial, upper transitional, and radial zones, with higher values noticed in the deeper transitional zone (Dunham et al 1986).…”

Section: Discussionmentioning

confidence: 97%

Distribution of lactate dehydrogenase in healthy and degenerative canine stifle joint cartilage

Walter

Spreng

Schmöckel

et al. 2007

Histochem Cell Biol

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In dogs, degenerative joint diseases (DJD) have been shown to be associated with increased lactate dehydrogenase (LDH) activity in the synovial fluid. The goal of this study was to examine healthy and degenerative stifle joints in order to clarify the origin of LDH in synovial fluid. In order to assess the distribution of LDH, cartilage samples from healthy and degenerative knee joints were investigated by means of light and transmission electron microscopy in conjunction with immunolabeling and enzyme cytochemistry. Morphological analysis confirmed DJD. All techniques used corroborated the presence of LDH in chondrocytes and in the interterritorial matrix of healthy and degenerative stifle joints. Although enzymatic activity of LDH was clearly demonstrated in the territorial matrix by means of the tetrazolium-formazan reaction, immunolabeling for LDH was missing in this region. With respect to the distribution of LDH in the interterritorial matrix, a striking decrease from superficial to deeper layers was present in healthy dogs but was missing in affected joints. These results support the contention that LDH in synovial fluid of degenerative joints originates from cartilage. Therefore, we suggest that (1) LDH is transferred from chondrocytes to ECM in both healthy dogs and dogs with degenerative joint disease and that (2) in degenerative joints, LDH is released from chondrocytes and the ECM into synovial fluid through abrasion of cartilage as well as through enhanced diffusion as a result of increased water content and degradation of collagen.

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Section: Discussionmentioning

confidence: 97%

Distribution of lactate dehydrogenase in healthy and degenerative canine stifle joint cartilage

Walter

Spreng

Schmöckel

et al. 2007

Histochem Cell Biol

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“…Viele tierexperimentelle Studien der vergangenen Jahre beschäftigten sich mit dem qualitativen Nachweis verschiedener geformter und ungeformter Anteile der Extrazellularmatrix, deren Funktion oft noch unbekannt ist [1,7,19]. Zunehmend rücken aber auch quantitative Untersuchungen zum belastungs-und altersabhängigen Metabolismus der Proteoglykane [24,32] und der kollagenen Fasern [9,[20][21][22][23][36][37][38][39] in den Mittelpunkt des Interesses.…”

Section: Methodsunclassified

“…Ein Vergleich zur Gewebedifferenzierung verschiedener primärer hyaliner Knorpelgewebe wurde somit möglich [30,31,[33][34][35]39]. from freshly slaughtered breeding animals at the abattoir under the supervision of a veterinary surgeon by means of a punch cylinder excision in the posterior third of the right condyle.…”

Section: Methodsunclassified

“…Many earlier studies based on animal experiments dealt with qualitative evidence of various shaped and unshaped components of the extracellular matrix, whose function is still unknown in many cases [1,7,19]. Increasingly, however, interest is being focused also on quantitative studies of the metabolism of proteoglycans [24,32] and collagen fibers [9,[20][21][22][23][36][37][38][39] in relation to stress and age.…”

Section: Introduction and Issuementioning

confidence: 99%

“…This permitted a comparison with the tissue differentiation of various primary hyaline cartilage tissues [30,31,[33][34][35]39].…”

Section: Introduction and Issuementioning

confidence: 99%

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Investigations into Growth and Differentiation in the Cartilage of the Condylar Process in the Domestic Pig

et al. 2004

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Quantitative measurement of human ageing using computer-aided radiographic texture analysis

Shamir¹

2013

Computer Methods in Biomechanics and Biomedical Engineering: Im

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Studying the process of ageing in humans is dependent on methods for quantitative measurement of the physiological differences between younger and older individuals. Although in many cases the chronological age is used as a measurement of ageing, it provides merely a rough approximation of the actual physiological age of a person. Here, we propose a computer method for measuring the physiological age of the human knee by using quantitative analysis of the radiographic Haralick bone texture. Experiments using longitudinal data from the Baltimore Longitudinal Study of Aging show that the radiographic Haralick texture correlates with the chronological age, and that the ageing of the knee as reflected by the tibial anisotropy and measured using the radiographic texture is not linear to the chronological age. The analysis reveals that the bone ages until the person gets to the age of ,40, then stabilises until the age of around 65, from where it continues to age. A period of rapid ageing was also detected around the age of 55. This shows that periods of rapid morphological changes of the knee occur not only during maturation, but also during ageing.

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Morphological and histochemical ageing changes in patellar articular cartilage of the rat

Cited by 6 publications

References 16 publications

Distribution of lactate dehydrogenase in healthy and degenerative canine stifle joint cartilage

Distribution of lactate dehydrogenase in healthy and degenerative canine stifle joint cartilage

Investigations into Growth and Differentiation in the Cartilage of the Condylar Process in the Domestic Pig

Quantitative measurement of human ageing using computer-aided radiographic texture analysis

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