The investigation entitled "Assessment of various plant growth substances on germination rates and seedling vigor in acid lime seedlings (Citrus aurantifolia Swingle) PDKV Lime" was carried out at All India Coordinated Research Project on fruits, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, during the years 2020-21 and 2021-22. The experiment was laid out in Randomized block design followed by pooled over-season analysis with fourteen treatments, including a check treatment, these treatments were replicated three times. The treatments included variations in concentrations and a combination of gibberellic acid, 6-benzyl adenine, brassinolide, and urea. The results indicated significant variations among the treatments under shade net conditions. The least number of days for first germination (5.67 days), 50 percent germination (13.00 days), and complete germination (17.50 days) were observed. However, maximum values for germination percentage (96.83%), seed vigor index-I (1450.28), seed vigor index II (36.52), root length (7.31 cm), and shoot length (7.69 cm) was recorded with treatment T13 (GA3 50 ppm + 6 BA 25 ppm + Brassinolide 2 ppm).