Morphological and genetic characterization of Echinococcus granulosus in the Slovak Republic

Turčeková, Ľ.; Šnábel, Viliam; D’Amelio, Stefano; Busi, Marina; Dubinský, P.

doi:10.1016/s0001-706x(02)00229-2

Cited by 46 publications

(34 citation statements)

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“…Five of the 18 Slovak isolates displayed 1-2 polymorphic sites compared to most common variant circulating in the region, and have been provisionally designated as G7A-C microvariants. Consistently with this study, only E. canadensis G7 was documented in Slovak sheep, cattle and humans in previous reports [22][23][24].…”

Section: Discussionsupporting

confidence: 93%

A molecular survey of Echinococcus granulosus sensu lato in central-eastern Europe

et al. 2016

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Open Life Sci. 2016; 11: 524-532 while infections with E. granulosus s.s. which are highly infectious for humans are more commonly encountered in Romania and Hungary.Keywords: Echinococcus granulosus, genotype, DNA sequences, human, pig, central-eastern Europe IntroductionThe larval stages of the tapeworm Echinococcus granulosus sensu lato (s.l.) are the causative agents of cystic echinococcosis (CE), one of the most important cestode infections causing morbidity and mortality in humans and significant economic losses in livestock. Around one million or more people are currently suffering from CE globally and the financial burden of the disease on the livestock industry is substantial, with up to two billion dollars lost annually [1]. Recent molecular phylogenetic analyses have revealed that E. granulosus is a complex of spesies/genotypes with at least five different species: E. granulosus sensu stricto (s.s.) (genotypes G1-G3), Echinococcus equinus (G4), Echinococcus ortleppi (G5), Echinococcus canadensis (G6-G10), and Echinococcus felidis ("lion strain") [2][3][4].CE remains one of the most important helminth zoonoses in many regions of Europe, especially the Mediterranean littoral (Spain, Italy, Greece and Turkey) and southeastern countries such as Bulgaria and Romania. The disease seems to be less prevalent in the UK, Central Europe, the Baltic States and the Scandinavian countries [5,6]. Surveys that addressed the incidence of CE over the last decade from central-eastern Europe has reported a relatively greater incidence in Romania (average incidence annual rate was 0.29 per 100,000 population) and Ukraine (0.30), whereas in Hungary (0.07), Poland (0.08) and Slovakia (0.10) lower numbers of human cases were documented [7]. This also is due to the fact that E. granulosus s.s., which is responsible for the majority Abstract: Central-eastern Europe is an endemic region for cystic echinococcosis where multiple species of intermediate hosts are commonly infected with Echinococcus granulosus sensu lato tapeworms of major medical and veterinary importance. Investigations of the genetic variation of 25 Echinococcus isolates from five countries (Slovakia, Romania, Ukraine, Hungary, Poland) were undertaken using three mitochondrial DNA markers. The 18 isolates from pigs derived from Slovakia and Ukraine and the four human isolates from Slovakia, Poland and Ukraine were identified as E. canadensis G7, whereas the three human isolates from Romania and Hungary were classified as E. granulosus sensu stricto G1. This study reports the first confirmed human case of E. granulosus s.s. in Hungary. The haplotype G7A with two polymorphic sites relative to the most common regional variant of E. canadensis G7 was recorded in both pigs from Ukraine and in a single pig isolate from Slovakia. The results of this study support the circumstantial evidence that E. canadensis G7 with low infectivity for humans is highly prevalent in the northern parts of the region (Poland, Slovakia, forest-steppe zone of Ukraine),

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Section: Discussionsupporting

confidence: 93%

A molecular survey of Echinococcus granulosus sensu lato in central-eastern Europe

et al. 2016

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“…Various enzymes have been tested in order to learn about evolution ecology and population genetics of cestodes (Okamoto et al 1995, Siles-Lucas et al. 1996, Turèeková et al 2003). In the present study no differences in the migration of 5 isozyme profiles (ARK, GOT, G3PD, GPI, and MPI) were found suggesting homozygotic profiles.…”

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Isozyme analysis of Taenia solium isolates from Mexico and Colombia

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Souza

et al. 2003

Mem. Inst. Oswaldo Cruz

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“…This second study suggested that, based on nad1 sequences, pigs (56 isolates examined) and humans (4 isolates examined) were infected with the G7 genotype or pig strain. Separate isoenzyme and DNA-based investigations of E. granulosus isolates (Snabel et al 2000 ;Turcekova et al 1998Turcekova et al , 2003, using RAPDs, nad1 sequence comparisons and PCR-RFLP analysis of the nuclear ITS1 region, have provided additional evidence for the almost exclusive presence of the G7 genotype in Slovakia. DNA from an isolate of E. granulosus taken from a wild boar (Sumy region, Ukraine) had identical nad1 sequence to the G7 genotype earlier found in pigs from the same region (Kedra et al 2000 b).…”

Section: Molecular Genetic Analysis Of Human Cystic Echinococcosis Camentioning

confidence: 99%

Molecular epidemiology of cystic echinococcosis

2003

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Echinococcus granulosus exhibits substantial genetic diversity that has important implications for the design and development of vaccines, diagnostic reagents and drugs effective against this parasite. DNA approaches that have been used for accurate identification of these genetic variants are presented here as is a description of their application in molecular epidemiological surveys of cystic echinococcosis in different geographical settings and host assemblages. The recent publication of the complete sequences of the mitochondrial (mt) genomes of the horse and sheep strains of E. granulosus and of E. multilocularis, and the availability of mt DNA sequences for a number of other E. granulosus genotypes, has provided additional genetic information that can be used for more in depth strain characterization and taxonomic studies of these parasites. This very rich sequence information has provided a solid molecular basis, along with a range of different biological, epidemiological, biochemical and other molecular-genetic criteria, for revising the taxonomy of the genus Echinococcus. This has been a controversial issue for some time. Furthermore, the accumulating genetic data may allow insight to several other unresolved questions such as confirming the occurrence and precise nature of the E. granulosus G9 genotype and its reservoir in Poland, whether it is present elsewhere, why the camel strain (G6 genotype) appears to affect humans in certain geographical areas but not others, more precise delineation of the host and geographic ranges of the genotypes characterised to date, and whether additional genotypes of E. granulosus remain to be identified.

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Morphological and genetic characterization of Echinococcus granulosus in the Slovak Republic

Cited by 46 publications

References 19 publications

A molecular survey of Echinococcus granulosus sensu lato in central-eastern Europe

A molecular survey of Echinococcus granulosus sensu lato in central-eastern Europe

Isozyme analysis of Taenia solium isolates from Mexico and Colombia

Molecular epidemiology of cystic echinococcosis

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