Morphological and Functional Alterations in Glycerol Preserved Rat Aortic Allografts

Fahner, P J; Idu, Mirza M.; Legemate, Dink A.; vanBavel, Ed; Borstlap, J; Pfaffendorf, M.; Marle, J. van; Gulik, Thomas M. van

doi:10.1177/039139880402701111

Cited by 17 publications

(23 citation statements)

References 34 publications

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“…Each sample was cut open longitudinally and dehydrated in graded solutions of glycerol/saline of 50, 75, 87 and 100% for 1 h each to clear the specimen, permitting an accurate light scattering measurement. Note that glycerol treatment did not cause distortion of the tissue’s shape or structure [51,53]. The samples were then unfolded and SALS measurements were conducted over the entire tissue by raster scanning.…”

Section: Methodsmentioning

confidence: 99%

Altered structural and mechanical properties in decellularized rabbit carotid arteries

et al. 2009

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Recently, major achievements in creating decellularized whole tissue scaffolds have drawn considerable attention to decellularization as a promising approach for tissue engineering. Decellularized tissues are expected to have mechanical strength and structure similar to the native tissues from which they are derived. However, numerous studies have shown that mechanical properties change after decellularization. Often, tissue structure is observed by histology and electron microscopy, but the structural alterations that may have occurred are not always evident. Here, a variety of techniques were used to investigate changes in tissue structure and relate them to altered mechanical behavior in decellularized rabbit carotid arteries. Histology and scanning electrom microscopy revealed that major extracellular matrix components were preserved and fibers appeared intact, although collagen appeared looser and less crimped after decellularization. Transmission electrom microscopy confirmed the presence of proteoglycans (PG), but there was decreased PG density and increased spacing between collagen fibrils. Mechanical testing and opening angle measurements showed that decellularized arteries had significantly increased stiffness, decreased extensibility and decreased residual stress compared with native arteries. Small-angle light scattering revealed that fibers had increased mobility and that structural integrity was compromised in decellularized arteries. Taken together, these studies revealed structural alterations that could be related to changes in mechanical properties. Further studies are warranted to determine the specific effects of different decellularization methods on the structure and performance of decellularized arteries used as vascular grafts.

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Section: Methodsmentioning

confidence: 99%

Altered structural and mechanical properties in decellularized rabbit carotid arteries

et al. 2009

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“…Previous in vitro experiments in our laboratory showed that this protocol resulted in optimal glycerol preservation of arterial allografts [18] .…”

Section: Preparation Of Glycerol-preserved Allograftsmentioning

confidence: 87%

“…The fact that glycerol preservation results in a tissue matrix without living cells diminishes immunogenicity of the graft. Previous experiments in our laboratory demonstrated that glycerol preservation of the rat aorta preserves the biomechanical characteristics and architecture of the vessel wall [18] .…”

Section: Introductionmentioning

confidence: 87%

Glycerol-Preserved Arterial Allografts Evaluated in the Infrarenal Rat Aorta

Fahner

Idu²,

Gulik³

et al. 2008

Eur Surg Res

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Background: Vascular transplantation has become an alternative for prosthetic grafts. Suitable storage methods for vascular allografts are therefore necessary. For small caliber arterial allografts, cryopreservation and cold storage showed discouraging results. Since glycerol preservation proved effective for the storage of skin allografts, this preservation method was investigated for vascular allografts using a rat aortic transplantation model. Methods: Glycerol-preserved allografts (GA) were transplanted to the infrarenal aorta (n = 18) in Wistar rats. A control group (n = 18) underwent immediate autotransplantation (AU) of an equal length of aorta. Results: Cumulative graft patency at 90 days’ follow-up was 93% for AU and 78% for GA (ns). No aneurysm formation was detected in both groups. Intraluminal endothelial cell coverage, integrity of the media and smooth muscle cell repopulation were comparable in both groups. Intimal thickness was less in GA than in AU and inflammatory reaction in the adventitia was diminished in GA. Conclusion: GA were successfully grafted with acceptable patency rates compared to autografts, while intima hyperplasia and adventitial inflammatory reaction were less.

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“…LPPs were measured in accordance with the method of Basu et al 4 and Fahner et al 9 One end of the laser-treated carotid segment was sealed by ligation, and the vessel lumen was filled with 0.9% saline solution containing MB as a visual indicator at 99 mL/h. The other end was ligated around a hollow needle that was connected to a rate-controlled syringe infusion pump and a pressure controller (Braun Medical, Melsungen, Germany) through an interposed T-valve.…”

Section: Methodsmentioning

confidence: 99%

Optimization of Suture-Free Laser-Assisted Vessel Repair by Solder-Doped Electrospun Poly(ε-caprolactone) Scaffold

et al. 2010

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Poor welding strength constitutes an obstacle in the clinical employment of laser-assisted vascular repair (LAVR) and anastomosis. We therefore investigated the feasibility of using electrospun poly(ε-caprolactone) (PCL) scaffold as reinforcement material in LAVR of medium-sized vessels. In vitro solder-doped scaffold LAVR (ssLAVR) was performed on porcine carotid arteries or abdominal aortas using a 670-nm diode laser, a solder composed of 50% bovine serum albumin and 0.5% methylene blue, and electrospun PCL scaffolds. The correlation between leaking point pressures (LPPs) and arterial diameter, the extent of thermal damage, structural and mechanical alterations of the scaffold following ssLAVR, and the weak point were investigated. A strong negative correlation existed between LPP and vessel diameter, albeit LPP (484 ± 111 mmHg) remained well above pathophysiological pressures. Histological analysis revealed that thermal damage extended into the medial layer with a well-preserved internal elastic lamina and endothelial cells. Laser irradiation of PCL fibers and coagulation of solder material resulted in a strong and stiff scaffold. The weak point of the ssLAVR modality was predominantly characterized by cohesive failure. In conclusion, ssLAVR produced supraphysiological LPPs and limited tissue damage. Despite heat-induced structural/mechanical alterations of the scaffold, PCL is a suitable polymer for weld reinforcement in medium-sized vessel ssLAVR.

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Morphological and Functional Alterations in Glycerol Preserved Rat Aortic Allografts

Cited by 17 publications

References 34 publications

Altered structural and mechanical properties in decellularized rabbit carotid arteries

Altered structural and mechanical properties in decellularized rabbit carotid arteries

Glycerol-Preserved Arterial Allografts Evaluated in the Infrarenal Rat Aorta

Optimization of Suture-Free Laser-Assisted Vessel Repair by Solder-Doped Electrospun Poly(ε-caprolactone) Scaffold

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