Morphological and electrophysiological characterization of layer III cells of the medial entorhinal cortex of the rat

Gloveli, Tengis; Schmitz, Dietmar; Empson, Ruth M.; Dugladze, Tamar; Heinemann, Uwe

doi:10.1016/s0306-4522(96)00494-0

Cited by 101 publications

(128 citation statements)

References 44 publications

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“…Representative voltage traces from the three primary neuronal types found in Layers II and III of the mEC are shown in Figure 1, panels Aa-Ac. As has been reported previously, these neuronal subtypes can be differentiated by the degree of "sag" in their membrane potential in response to hyperpolarizing current injection ,Jones, 1994,Dickson et al, 1997,Gloveli et al, 1997,van der Linden and Lopes da Silva, 1998. Neurons either showed a large sag potential (Sag, Aa.…”

Section: Electrophysiological and Histological Characterization Of Mementioning

confidence: 74%

“…Whole cell patch-clamp recordings were acquired in current-clamp and voltage-clamp mode using an Axopatch 1D or 200B amplifier and the CLAMPEX 9 software package interfaced to a Digidata 1322A data acquisition board (Axon Instruments, Union City, CA). Neurons were initially recorded in currentclamp mode (Vm = −70 mV) and neuron identity was determined by electroresponsive membrane properties , Jones, 1994, Dickson et al, 1997, Gloveli et al, 1997, van der Linden and Lopes da Silva, 1998. In current clamp mode, series resistance was compensated by ≥ 80%.…”

Section: Whole Cell Patch-clamp Recordings In Adult Mec Slicesmentioning

confidence: 99%

“…As in previous studies , Jones, 1994, Dickson et al, 1997, Gloveli et al, 1997 der Linden and Lopes da Silva, 1998), slices were processed for biocytin in order to assess the location and morphology of these neurons, and labeled neurons were correlated with their electrophysiology . When examined in this manner, S neurons invariably had a stellate morphology characterized by a broad dendritic arborization in Layer I and a cell body commonly found in Layer II (12 of 13 neurons).…”

Section: Electrophysiological and Histological Characterization Of Mementioning

confidence: 99%

“…Although the electroresponsive membrane properties and morphologies of neurons in the superficial layers of the mEC have been previously characterized by others , Jones, 1994, Dickson et al, 1997, Gloveli et al, 1997, van der Linden and Lopes da Silva, 1998, Heinemann et al, 2000, we performed these experiments in order to classify neurons before examination of the presence and contributions of their postsynaptic KARs. Consistent with numerous studies, we identified three primary neuronal classes.…”

Section: Superficial Layer Neurons Of the Rat Medial Entorhinal Cortexmentioning

confidence: 99%

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Differential contribution of kainate receptors to excitatory postsynaptic currents in superficial layer neurons of the rat medial entorhinal cortex

2007

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Although in situ hybridization studies have revealed the presence of kainate receptor (KAR) mRNA in neurons of the rat medial entorhinal cortex (mEC), the functional presence and roles of these receptors are only beginning to be examined. To address this deficiency, whole cell voltage clamp recordings of locally evoked EPSCs were made from mEC layer II and III neurons in combined entorhinal cortex -hippocampal brain slices. Three types of neurons were identified by their electroresponsive membrane properties, locations, and morphologies: stellate-like "Sag" neurons in layer II (S), pyramidal-like "No Sag" neurons in layer III (NS), and "Intermediate Sag" neurons with varied morphologies and locations (IS). Non-NMDA EPSCs in these neurons were composed of two components, and the slow decay component in NS neurons had larger amplitudes and contributed more to the combined EPSC than did those observed in S and IS neurons. This slow component was mediated by KARs and was characterized by its resistance to either GYKI 52466 (100 μM) or NBQX (1 μM), relatively slow decay kinetics, and sensitivity to CNQX (10-50 μM). KAR mediated EPSCs in pyramidal-like NS neurons contributed significantly more to the combined non-NMDA EPSC than did those from S and IS neurons. Layer III neurons of the mEC are selectively susceptible to degeneration in human temporal lobe epilepsy (TLE) and animal models of TLE such as kainate-induced status epilepticus. Characterizing differences in the complement of postsynaptic receptors expressed in injury prone versus injury resistant mEC neurons represents an important step toward understanding the vulnerability of layer III neurons seen in TLE.

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Section: Electrophysiological and Histological Characterization Of Mementioning

confidence: 74%

Section: Whole Cell Patch-clamp Recordings In Adult Mec Slicesmentioning

confidence: 99%

Section: Electrophysiological and Histological Characterization Of Mementioning

confidence: 99%

Section: Superficial Layer Neurons Of the Rat Medial Entorhinal Cortexmentioning

confidence: 99%

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Differential contribution of kainate receptors to excitatory postsynaptic currents in superficial layer neurons of the rat medial entorhinal cortex

2007

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“…The multimodal sensory information received by the EC is processed and via output neurons in the superficial layers projected to the hippocampus (Steward and Scoville 1976;Gloveli et al 1997). Hippocampal projections to the deep layers of EC contribute return connectivity (Kloosterman et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Coexpression of vesicular glutamate transporters 1 and 2, glutamic acid decarboxylase and calretinin in rat entorhinal cortex

Wouterlood¹,

Canto

Aliane³

et al. 2007

Brain Struct Funct

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We studied the distribution and coexpression of vesicular glutamate transporters (VGluT1, VGluT2), glutamic acid decarboxylase (GAD) and calretinin (CR, calcium-binding protein) in rat entorhinal cortex, using immunofluorescence staining and multichannel confocal laser scanning microscopy. Images were computer processed and subjected to automated 3D object recognition, colocalization analysis and 3D reconstruction. Since the VGluTs (in contrast to CR and GAD) occurred in fibers and axon terminals only, we focused our attention on these neuronal processes. An intense, punctate VGluT1-staining occurred everywhere in the entorhinal cortex. Our computer program resolved these punctae as small 3D objects. Also VGluT2 showed a punctate immunostaining pattern, yet with half the number of 3D objects per tissue volume compared with VGluT1, and with statistically significantly larger 3D objects. Both VGluTs were distributed homogeneously across cortical layers, with in MEA VGluT1 slightly more densely distributed than in LEA. The distribution pattern and the size distribution of GAD 3D objects resembled that of VGluT2. CR-immunopositive fibers were abundant in all cortical layers. In double-stained sections we noted ample colocalization of CR and VGluT2, whereas coexpression of CR and VGluT1 was nearly absent. Also in triple-staining experiments (VGluT2, GAD and CR combined) we noted coexpression of VGluT2 and CR and, in addition, frequent coexpression of GAD and CR. Modest colocalization occurred of VGluT2 and GAD, and incidental colocalization of all three markers. We conclude that the CR-containing axon terminals in the entorhinal cortex belong to at least two subpopulations of CRneurons: a glutamatergic excitatory and a GABAergic inhibitory.

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Morphological and electrophysiological characteristics of layer V neurons of the rat medial entorhinal cortex

et al. 2000

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Morphological and electrophysiological characterization of layer III cells of the medial entorhinal cortex of the rat

Cited by 101 publications

References 44 publications

Differential contribution of kainate receptors to excitatory postsynaptic currents in superficial layer neurons of the rat medial entorhinal cortex

Differential contribution of kainate receptors to excitatory postsynaptic currents in superficial layer neurons of the rat medial entorhinal cortex

Coexpression of vesicular glutamate transporters 1 and 2, glutamic acid decarboxylase and calretinin in rat entorhinal cortex

Morphological and electrophysiological characteristics of layer V neurons of the rat medial entorhinal cortex

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