Morphological and biochemical perturbations in rat erythrocytes following in vitro exposure to Fenvalerate and its metabolite

Prasanthi, K.; Muralidhara, .; Rajini, P.S.

doi:10.1016/j.tiv.2004.12.003

Cited by 59 publications

(31 citation statements)

References 40 publications

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“…Some pyrethroids affect the flow of erythrocyte membrane due to increased lipid peroxidation (Kale et al, 1999, Gabbianelli et al, 2002Nasuti et al, 2003). It is likely that the production of O 2 -or the direct action of pyrethroid on the production of GPx could be the cause of oxidative damage (Prasanthi et al, 2005;El Demerdash, 2007). The correlation between TBARS and AChE activity found in the present study (Figure 3) is similar that obtained by other authors (Ranjbar et al, 2002;Akhgari et al, 2003;Singh et al, 2007).…”

Section: Discussionsupporting

confidence: 90%

“…The results of the present study indicate that CAT activity decreased significantly in both pesticide applicators and non-pesticide applicators (P < 0.001). The available data on experimental animals , in vitro studies (Gultekin et al, 2000;Prasanthi et al, 2005) and in vivo studies (Ranjbar et al, 2002;Lopez et al, 2007) indicate that the enzymes associated with the antioxidant defence mechanism change under the influence of pesticides. These enzymes efficiently scavenge toxic free radicals and are partly responsible for protection against lipid peroxidation due to pesticide exposure (Banerjee et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

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Agrochemicals: Horticulture Use Conditions Determine Genotoxic Effects and Oxidative Damage in Rural Populations in Santa Fe, Argentina

Carballo¹,

Simoniello²,

Kleinsorge³

2011

Pesticides - The Impacts of Pesticides Exposure

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Agrochemicals: Horticulture Use Conditions Determine Genotoxic Effects and Oxidative Damage in Rural Populations in Santa Fe, Argentina

Carballo¹,

Simoniello²,

Kleinsorge³

2011

Pesticides - The Impacts of Pesticides Exposure

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“…It also interfered with glucose-6-phosphate dehydrogenase activity that are required for membrane integrity (Mayes, 1983;Champe et al, 2005;Ojo et al, 2006). Although erythrocytes are well equipped with several biological mechanisms to defend against intracellular oxidative stress (Prasanthi et al, 2005) they can be oxidatively damaged due to toxic chemicals and environmental pollutants (Uchendu et al, 2014). Another view of the mechanism of fragility is that the hydrocarbon component also interfered with the membrane sodium pump mechanism that maintains a low level of sodium ion concentration inside the cell.…”

Section: Discussionmentioning

confidence: 99%

The ameliorative effects of <i>Moringa oleifera</i> leaf extract on cardiovascular functions and osmotic fragility of wistar rats exposed to petrol vapour

Azeez¹,

Adah²,

Olaifa³

et al. 2017

Sokoto J. Vet. Sc.

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The ameliorative effects of Moringa oleifera leaf extract on cardiovascular functions and osmotic fragility of wistar rats exposed to petrol vapour OM Azeez*, SA Adah, FH Olaifa, A Basiru & R AbdulbakiUniversity of Ilorin, Ilorin, Nigeria *Correspondence: Tel.: +2348033858362; E-mail: misibisol@yahoo.com Abstract The present study was aimed at evaluating the ameliorating effects of Moringa oleifera extract compared to captopril and candesartan cilexetil on cardiovascular functions and osmotic fragility of rats exposed to petrol vapour. Twenty five adult male Wistar rats (130g-200g) were randomly grouped to five with five rats in a group. Group 1 (control) was not exposed to petrol fume. Groups 2 (petrol only), was exposed to petrol fume only. Groups 3, 4 and 5 were pretreated with Moringa oleifera extract (40mg/kg), captopril (25 mg/kg) and candesartan (16mg/kg), respectively before exposure to petrol vapour, 10 minutes every day for eight weeks. All groups were given feed and water ad-libitum. Petrol vapour was generated by using human compressor nebulizer adopted for rats and connected to fume chamber where the rats were kept. The pretreatment were administered by oral cannula. At the end of the exposure, 0.2ml of blood samples obtained from individual rat in each group were suspended in separate sets of Phosphate buffer saline (PBS) solution of decreasing concentrations. Erythrocyte osmotic fragility (EOF) was determined by spectrophotometer. Electrocardiography was done using EDAN 10. There was significant increase (p<0.05) in EOF of the rats exposed to petrol vapour only. However, Moringa oleifera, captopril and candesartan cilexetil significantly ameliorated this effect. There was no significant difference in the amelioration of Moringa oleifera and candesartan cilexetil. There was absence of p-wave and significant increase in heart rate observed in the electrocardiogram of petrol only group, this was significantly restored in the Moringa oleifera, captopril and the candesartan cilexetil group. The results showed that exposure to petrol vapour elevated EOF, resulted in atria arrhythmia and increased heart rate. These effects were ameliorated by pretreatment with Moringa oleifera, captopril and candesartan cilexetil. The amelioration in Moringa oleifera was comparable with that of candesartan cilexetil suggesting that Moringa oleifera may be an Angiotensin II receptor blocker.

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“…This is also the case of pyrethroids (Meeker et al, 2009), and this is also the reason for research on the molecular mechanisms of these compounds. Toxic effects of the pyrethroids cypermethrin, deltamethrin and fenvalerate were demonstrated not only on the nervous system (Wolansky and Harrill, 2008), but also on various types of mammalian somatic cells cultured in vitro (Prasanthi et al, 2005) and even on gametes -sperm (Yuan et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Pyrethroids cypermethrin, deltamethrin and fenvalerate have different effects on in vitro maturation of pig oocytes at different stages of growth

Petr

Eva

Žalmanová

et al. 2013

Animal

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Pesticides can significantly harm reproduction in animals and people. Pyrethroids are often used as insecticides, and their toxicity for mammals is considered to be low. However, cypermethrin, deltamethrin and fenvalerate -as potent specific inhibitors of protein phosphatase calcineurin -can influence the meiosis of mammalian oocytes. The objective of this study was to evaluate the effects of these pyrethroids on the in vitro maturation of pig oocytes at different levels of meiotic competence. Under the tested concentrations, cypermethrin, deltamethrin and fenvalerate neither had a significant effect on the viability of oocytes nor did they induce significant degeneration of oocytes. However, these pyrethroids significantly affected meiotic maturation. The effects depended on the stage of meiotic competence of the oocytes. Maturation of growing pig oocytes with partial meiotic competence was induced. On the other hand, in fully grown pig oocytes with full meiotic competence, maturation in vitro was delayed. The specificity of these effects was further supported by the same effect of non-pyrethroidal inhibitors of calcineurin -cyclosporin A or hymenistatin I -on the maturation of oocytes with different levels of meiotic competence. However, pyrethroids, which do not inhibit calcineurin -allethrin or permethrin -had no effect on pig oocyte maturation. We demonstrated a significant effect of pyrethroids on the maturation of mammalian oocytes under in vitro conditions. This indicates that exposure to these substances could affect the fertility of people or animals.

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Morphological and biochemical perturbations in rat erythrocytes following in vitro exposure to Fenvalerate and its metabolite

Cited by 59 publications

References 40 publications

Agrochemicals: Horticulture Use Conditions Determine Genotoxic Effects and Oxidative Damage in Rural Populations in Santa Fe, Argentina

Agrochemicals: Horticulture Use Conditions Determine Genotoxic Effects and Oxidative Damage in Rural Populations in Santa Fe, Argentina

The ameliorative effects of <i>Moringa oleifera</i> leaf extract on cardiovascular functions and osmotic fragility of wistar rats exposed to petrol vapour

Pyrethroids cypermethrin, deltamethrin and fenvalerate have different effects on in vitro maturation of pig oocytes at different stages of growth

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