Morphological and anatomical characteristics of exserted stigma sterility and the location and function of SlLst (Solanum lycopersicum Long styles) gene in tomato

Cheng, Mozhen; Gong, Chao; Zhang, Bo; Qü, Wei; Qi, Huayu; Chen, Xiuling; Wang, Xingyuan; Zhang, Yao; Liu, Jiayin; Ding, Xiaodong; Qiu, Yefeng; Wang, Aoxue

doi:10.1007/s00122-020-03710-0

Cited by 15 publications

(15 citation statements)

References 49 publications

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“…The rates of falling flowers and fruit of glycyrrhiza uralensis are negatively correlated with the IAA level and positively correlated with the ABA level [41]. IAA played a major role, while ABA had the opposite effect on stigma exertion in tomato [42]. In maize, the contents of IAA and GA were positively correlated with the maximum seed filling rate, seed weight, and mean filling rate, while ABA was negatively correlated [43].…”

Section: Discussionmentioning

confidence: 97%

Genome-Wide Identification and Expression Analysis of NCED Gene Family in Pear and Its Response to Exogenous Gibberellin and Paclobutrazol

Yuan

Quan

Zhang

et al. 2023

IJMS

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The 9-cis-epoxycarotenoid dioxygenase (NCED) is a key enzyme for the process of ABA synthesis that plays key roles in a variety of biological processes. In the current investigation, genome-wide identification and comprehensive analysis of the NCED gene family in ‘Kuerle Xiangli’ (Pyrus sinkiangensis Yu) were conducted using the pear genomic sequence. In total, nineteen members of PbNCED genes were identified from the whole genome of pear, which are not evenly distributed over the scaffolds, and most of which were focussed in the chloroplasts. Sequence analysis of promoters showed many cis-regulatory elements, which presumably responded to phytohormones such as abscisic acid, auxin, etc. Synteny block indicated that the PbNCED genes have experienced strong purifying selection. Multiple sequence alignment demonstrated that these members are highly similar and conserved. In addition, we found that PbNCED genes were differentially expressed in various tissues, and three PbNCED genes (PbNCED1, PbNCED2, and PbNCED13) were differentially expressed in response to exogenous Gibberellin (GA3) and Paclobutrazol (PP333). PbNCED1 and PbNCED13 positively promote ABA synthesis in sepals after GA3 and PP333 treatment, whereas PbNCED2 positively regulated ABA synthesis in ovaries after GA3 treatment, and PbNCED13 positively regulated ABA synthesis in the ovaries after PP333 treatment. This study was the first genome-wide report of the pear NCED gene family, which could improve our understanding of pear NCED proteins and provide a solid foundation for future cloning and functional analyses of this gene family. Meanwhile, our results also give a better understanding of the important genes and regulation pathways related to calyx abscission in ‘Kuerle Xiangli’.

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Section: Discussionmentioning

confidence: 97%

Genome-Wide Identification and Expression Analysis of NCED Gene Family in Pear and Its Response to Exogenous Gibberellin and Paclobutrazol

Yuan

Quan

Zhang

et al. 2023

IJMS

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“…These data suggest that a favorable SP can be harnessed by breeding, although few QTLs for this trait are known. For many years, the only known gene controlling SP was Se2.1 [ 23 ], but recently, other positions have been identified [ 46 ] together with new candidate genes [ 12 , 25 ]. The SP variability present in landraces may be exploited to unravel new QTLs for this trait by biparental or linkage disequilibrium-based QTL mapping.…”

Section: Discussionmentioning

confidence: 99%

“…The molecular basis for the control stigma position (SP) has started to be elucidated; a major quantitative trait locus (QTL) was positioned on the long arm of Chr2 [ 22 ] and the underlying gene, later identified as encoding a transcription factor that regulates cell elongation in developing styles [ 23 ]. Other genes involved in SP control have recently been suggested by transcriptional analyses [ 20 , 24 ] or by validation of candidates [ 25 , 26 ]. In cultivated tomato, a tendency toward an exserted SP is retained by many cultivated genotypes, especially landraces and primitive cultivars [ 27 , 28 ]; frequently, an increase in stigma exsertion is observable in these genotypes under heat stress [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Dynamics of Fertility-Related Traits in Tomato Landraces under Mild and Severe Heat Stress

Farinon¹,

Picarella²,

Mazzucato³

2022

Plants

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Studies on the reproductive dynamics under heat stress are crucial to breed more tolerant cultivars. In tomato, cultivars, breeding lines, and wild species have been evaluated for their response to heat stress. Here, we addressed the study to a panel of selected landraces representing traditional genotypes that usually show high adaptation to local environments. In two experiments, spaced by 12 years, we set-up an identical experimental design with plants transplanted at two different dates to expose the second field to thermic stress with natural fluctuations. Such a strategy resulted in both a mild and severe stress in the two years. The landraces showed wide variation for both vegetative and reproductive traits; all traits were affected by heat, mostly with a significant Genotype*Environment interaction. A high broad-sense heritability was estimated for plant height, stigma position, pollen viability, and fruit weight. Low heritability estimates were found for the number of flowers, fruit set, and yield. Despite the interaction, traits recorded under control and heat conditions were positively correlated. Multivariate analysis located the genotypes in a topography that was stable under all conditions, except under the harshest temperatures. The study revealed that landraces present a wide variability for the response of reproductive traits to thermic challenges and that such a variation could be useful to dissect the traits with higher heritability and identify quantitative trait loci for breeding more resilient varieties.

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“…Endogenous hormones gibberellin (GA 3 ), abscisic acid (ABA) and auxin (IAA) were extracted and quantified according to the method of Cheng ( Cheng et al, 2021 ). Starch granules were stained by iodine solution (1% KI and 0.5% I 2 )( Taiz and Zeiger, 2010 ).…”

Section: Methodsmentioning

confidence: 99%

Cool–Warm Temperature Stratification and Simulated Bird Digestion Optimize Removal of Dormancy in Rosa rugosa Seeds

et al. 2022

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Rosa rugosa Thunb. has been explored multi-function in medicinal, edible, cosmetic, ornamental and ecological etc. However, R. rugosa natural populations have recently declined substantially in China, besides of global climate change, this species also has the defect of limiting the reproduction of itself such as the hard-to-release seed dormancy. In this study, only 30% of R. rugosa seeds were viable, and the others were incompletely developed or diseased seeds. Without stratification, morphologically complete viable seeds imbibed water but those seeds could not germinate even after seed husk removal under suitable condition to exhibit a physiological dormancy. After cold (4°C) and warm (18 ± 2°C) stratification, macromolecular substances containing carbon or nitrogen accumulated, and respiration, antioxidant enzyme activity, and gibberellin (GA3) /abscisic acid (ABA) and auxin (IAA)/ABA ratios increased significantly in seeds. Water absorption also increased as endocarps softened. Thus, physiological dormancy of seed was broken. Although warm and cold stratification increased separation between endocarp and embryo, the endocarp binding force was removed insufficiently, because only 10.20% of seeds germinated. Therefore, stratified seeds were treated with simulated bird digestion. Then, folds and cracks in loosened endocarps increased permeability, and water absorption rate increased to 64.43% compare to 21.14% in cold and warm stratification treatment. With simulated digestion, 24.20% of radicles broke through the endocarp with plumules and cambiums to develop into seedlings. Thus, the seed dormancy type of R. rugosa is physiological as seeds imbibed water and possessed fully developed embryos with a low growth potential in combination with a mechanical constraint from the endocarp. Cold stratification helped remove physiological dormancy, and additional warm stratification accelerated the process. The optimal stratification treatment was 4°C for 45 days followed by 18 ± 2°C for 15 days. After warm and cold stratification, simulated bird digestion broke the mechanical constraint from the seed covering layers. Based on this research, production of R. rugosa seedlings can be greatly increased to help protect the species from further declines.

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Morphological and anatomical characteristics of exserted stigma sterility and the location and function of SlLst (Solanum lycopersicum Long styles) gene in tomato

Cited by 15 publications

References 49 publications

Genome-Wide Identification and Expression Analysis of NCED Gene Family in Pear and Its Response to Exogenous Gibberellin and Paclobutrazol

Genome-Wide Identification and Expression Analysis of NCED Gene Family in Pear and Its Response to Exogenous Gibberellin and Paclobutrazol

Dynamics of Fertility-Related Traits in Tomato Landraces under Mild and Severe Heat Stress

Cool–Warm Temperature Stratification and Simulated Bird Digestion Optimize Removal of Dormancy in Rosa rugosa Seeds

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